Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,760,848	T→C	67.6%	V80A (GTG→GCG)	yrdC →	dsRNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,760,848	0	T	C	67.6%	73.9 / 41.9	86	V80A (GTG→GCG)	yrdC	dsRNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (15/13);  new base C (36/22);  total (51/35)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGACAA  >  minE/2760782‑2760916                                                                   |                                                                     tGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGc                                                                    >  1:411601/1‑69 (MQ=255) tGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGc                                                                    >  1:2718308/1‑69 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:3386591/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2363331/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2458767/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2492263/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2513726/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2573513/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:218035/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:839470/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2577448/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2133609/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2087600/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:1893689/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2766474/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:3215605/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:1368092/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2880236/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:2922093/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:3042222/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:306862/1‑68 (MQ=255)      ttAATCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCg                                                                >  1:363324/1‑68 (MQ=255)      ttAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCg                                                                >  1:2850512/1‑68 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:3414437/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2838599/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:344480/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2558602/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:3079589/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2663355/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:459331/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2851150/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2850958/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2793584/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:161684/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2338152/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:1649086/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:998594/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:1766700/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:1022107/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:1808114/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:203824/68‑1 (MQ=255)        aaTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2054368/68‑1 (MQ=255)        aaTCGCGGCAAAATACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:2397528/68‑1 (MQ=255)         aTCGCGGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:1892762/67‑1 (MQ=255)                                tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                                     <  1:2128244/69‑1 (MQ=255)                                tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                                     <  1:2709055/69‑1 (MQ=255)                                tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                                     >  1:569157/1‑69 (MQ=255)                                tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                                     >  1:1428708/1‑69 (MQ=255)                                tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                                     <  1:2528885/69‑1 (MQ=255)                                tAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAgg                                     <  1:954562/69‑1 (MQ=255)                                  aaCCCTATATTGATGACACCATGCTGACTGACGCGCAGCGTg                                                              <  1:1033931/42‑1 (MQ=38)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAGCCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:3206833/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCGGCTGGc                                         >  1:1868901/1‑53 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCg                                              >  1:3248645/1‑48 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:1085825/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:408570/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:3395530/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:987704/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:2453457/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:1610460/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:1640715/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:1687310/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:2849795/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:2136107/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:81117/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:2233065/1‑69 (MQ=255)                                            tgatgaCACCATGCTGACTGACGCGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:576067/1‑69 (MQ=255)                                             gatgaCACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:3069449/1‑68 (MQ=255)                                             gatgaCACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCtt                         >  1:804050/1‑68 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAg                                      >  1:2730348/1‑43 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg            >  1:547975/1‑69 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg            >  1:999676/1‑69 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg            >  1:2645545/1‑69 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg            >  1:1066450/1‑69 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg            >  1:2709269/1‑69 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgcg            >  1:3165321/1‑69 (MQ=255)                                                         ttGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCcgc             >  1:2709380/1‑68 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGTCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:359259/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:3118256/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:3338020/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:227753/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:594048/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:2261517/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:219508/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:2152910/68‑1 (MQ=255)                                                                  gTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:1513303/68‑1 (MQ=255)                                                                   tGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGacaa  <  1:366302/69‑1 (MQ=255)                                                                   tGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGacaa  <  1:378599/69‑1 (MQ=255)                                                                   tGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGacaa  <  1:3039598/69‑1 (MQ=255)                                                                   tGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGacaa  <  1:1599483/69‑1 (MQ=255)                                                                   tGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGac    <  1:1595658/67‑1 (MQ=255)                                                                   |                                                                     TGATTTTAATCGCAGCAAATTACGAGCAGCTTAAACCCTATATTGATGACACCATGTTGACTGACGTGCAGCGTGAAACCATTTTTTCCCGCTGGCCAGGTCCTGTCACCTTTGTCTTTCCCGCGCCTGCGACAA  >  minE/2760782‑2760916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑