| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,908,139 | T→C | 50.0% | K164K (AAA→AAG) | treR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,908,139 | 0 | T | C | 50.0% | ‑4.6 / 118.1 | 124 | K164K (AAA→AAG) | treR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (41/21); new base C (30/32); total (71/53) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
ACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCA > minE/2908073‑2908206 | acTGTGCGGCACGCCGAGATAACTGATATTACGATG‑CCCTGGTCATACAGCCGTTGCATCAGGATTTTg < 1:1756849/69‑1 (MQ=255) tgtgCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTg < 1:3178469/68‑1 (MQ=255) tgtgCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTg < 1:2063819/68‑1 (MQ=255) tgtgCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTg < 1:3257506/68‑1 (MQ=255) tgtgCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTg < 1:3207121/68‑1 (MQ=255) aagCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:1030996/3‑69 (MQ=255) aCGCCGAGATAGGTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:2196456/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:740528/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:3228526/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:29793/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:1224592/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:2686897/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:2602683/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:250545/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:2341900/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:1458577/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:2164532/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:1597034/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCACt > 1:823466/1‑69 (MQ=255) aCGCCGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGATGCATCAGGATCTTGATTGCCCCt > 1:2515056/1‑69 (MQ=255) aCGCCGAGATAGCCGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCt > 1:445306/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTg > 1:261586/1‑60 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:460161/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:399885/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:2273217/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:1319195/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:2622180/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:1178214/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:334014/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:2045773/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:2910743/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:76710/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:1076738/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:1862027/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:1379940/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:1805378/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:1675246/1‑69 (MQ=255) aCGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCt > 1:2472591/1‑69 (MQ=255) aCGCAGAGATAGCTGATATTACGATGTCCCTGGTCATACAGCCGTTGCATCAGGATCTTGATTGccc > 1:1722214/1‑67 (MQ=255) aCTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCa > 1:287365/1‑67 (MQ=255) aCTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt > 1:435710/1‑68 (MQ=255) aCGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCgtcgtc < 1:313126/57‑1 (MQ=255) aCGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCgtcgtc < 1:693097/57‑1 (MQ=255) tcccTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCaaa < 1:47519/68‑1 (MQ=255) tcccTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCaaa < 1:2417114/68‑1 (MQ=255) tcccTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCaaa < 1:2944379/68‑1 (MQ=255) tcccTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCaaa < 1:1592960/68‑1 (MQ=255) tcccTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCaaa < 1:1192651/68‑1 (MQ=255) tcccTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCaaa < 1:1531092/68‑1 (MQ=255) tcccTGGTCATACAGCCGTTGCATCAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCaaa < 1:437165/68‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:661233/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:193021/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:2653904/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:1487548/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:723136/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:1425579/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:2361695/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:2435167/47‑1 (MQ=255) gTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCAt < 1:2393976/47‑1 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGc > 1:510892/1‑59 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:3281962/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:394358/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:73994/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:934629/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:919945/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:38939/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:3066765/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:1967556/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:2266355/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:2283985/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:1819408/1‑69 (MQ=255) cAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTc > 1:1141864/1‑69 (MQ=255) cAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCcagcag < 1:2753508/69‑1 (MQ=255) cAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCcagcag < 1:2329997/69‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:797446/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:1121910/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:1033882/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:1369026/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:1744395/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:1033145/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:1868191/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:1915499/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:101135/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:3063364/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:2277362/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:425113/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:2377369/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:350820/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:3388071/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:3379471/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:2524941/39‑1 (MQ=255) cAGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGa < 1:2566066/39‑1 (MQ=255) aGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGcc > 1:2955553/1‑47 (MQ=255) aGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCcagcag > 1:583726/1‑68 (MQ=255) aGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGa > 1:2083430/1‑69 (MQ=255) aGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGa > 1:481668/1‑69 (MQ=255) aGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGa > 1:1274783/1‑69 (MQ=255) aGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGa > 1:3380217/1‑69 (MQ=255) aGGATCTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGa > 1:2810281/1‑69 (MQ=255) ttttGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCa > 1:766017/1‑69 (MQ=255) | ACTGTGCGGCACGCCGAGATAACTGATATTACGATGCCCCTGGTCATACAGCCGTTGCATCAGGATTTTGATTGCCCCTTCGTCGTCATAACAGACCGAAGCAAAGCCTTTTGCGTCACGCGCCAGCAGAACCA > minE/2908073‑2908206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |