| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 520,562 | C→T | 32.3% | R194R (CGC→CGT) | uvrB → | excinulease of nucleotide excision repair, DNA damage recognition component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 520,562 | 0 | C | T | 32.3% | 100.9 / 58.0 | 114 | R194R (CGC→CGT) | uvrB | excinulease of nucleotide excision repair, DNA damage recognition component |
| Reads supporting (aligned to +/- strand): ref base C (38/39); new base T (27/10); total (65/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.83e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
CTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAC > minE/520497‑520626 | cTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGtt > 1:3305722/1‑69 (MQ=255)cTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGtt > 1:2717944/1‑69 (MQ=255)cTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGtt < 1:2402500/69‑1 (MQ=255)cTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGt > 1:2706053/1‑68 (MQ=255) ccGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTg < 1:2566545/68‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:231840/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:610793/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:618793/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:1989227/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:563711/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:2491579/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:2477726/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:938993/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:3070824/69‑1 (MQ=255) ggCGGAGCTGCAATACGCTCGTAATGATAAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:85660/69‑1 (MQ=255) gCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGGGGCGAGGt < 1:792562/68‑1 (MQ=255) cTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTtatagat < 1:1448385/69‑1 (MQ=255) cTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTgatagat < 1:2694312/69‑1 (MQ=255) cTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTgatagat < 1:561018/69‑1 (MQ=255) cTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTgatagat < 1:1442659/69‑1 (MQ=255) cTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTgatagat < 1:1419540/69‑1 (MQ=255) cTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTgatagat < 1:1665729/69‑1 (MQ=255) tcGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:2215728/2‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCTTGGCGAGGTGATAGATATCTTcc > 1:2386546/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:2202583/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:2214604/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:624212/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:2638683/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:2642750/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:723582/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:3205137/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:3139411/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTcc > 1:3121074/1‑69 (MQ=255) aCGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTAGCGAGGTGATAGATATc > 1:1574729/1‑65 (MQ=255) tAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGt < 1:2698424/48‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:952058/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:2781313/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:2785444/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:913889/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:477112/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:2083977/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:1859145/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGTGTTCGCGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:1273603/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:2542921/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:2728853/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:1489460/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:155918/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:1570460/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:1757829/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:1818306/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:3326202/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:1226602/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:2473243/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:2854333/68‑1 (MQ=255) gCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:2283809/68‑1 (MQ=255) cATTCCAGCGTGGTACTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGa < 1:1393462/67‑1 (MQ=255) aTTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGa < 1:2239064/66‑1 (MQ=255) ccAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGc > 1:2859821/1‑69 (MQ=255) ccAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGc > 1:1278110/1‑69 (MQ=255) gTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTcgcg > 1:1639108/1‑69 (MQ=255) gTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTcgcg > 1:2065037/1‑69 (MQ=255) gTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTcgcg > 1:21964/1‑69 (MQ=255) cTTTCCGTGTTCTTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2297207/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:89629/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:3067281/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:3394265/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2637610/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:304490/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2886858/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:1589035/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:279435/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2779300/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2750846/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:1909918/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2705504/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:1500123/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2704531/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:2108257/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:1045933/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:1486819/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTg > 1:3309847/1‑68 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTg > 1:1863194/1‑68 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTg > 1:2264648/1‑68 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTg > 1:348722/1‑68 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGCAGAATCGGATGACATTGAACTTCGCGTgg > 1:3204139/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGGTGATTGATATCTTCCCGGAAGAATCGGATGACATTGCACTTCGCGTgg > 1:1352764/1‑69 (MQ=255) cTTTCCGTGTTCGTGGCGAGCTGATTGATATCTTCCCGGCAGAAACGGATGACATTGCACTTCGCGTgg > 1:2810781/1‑69 (MQ=255) cTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:544084/1‑69 (MQ=255) cTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTgg > 1:3261914/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:1497372/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:2479230/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:1313074/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:1053655/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:161875/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:1993786/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:3327696/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:2483123/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:2743794/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:2961703/1‑69 (MQ=255) tCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAc > 1:2890420/1‑69 (MQ=255) | CTGCGCCGACTGGCGGAGCTGCAATACGCTCGTAATGATCAAGCATTCCAGCGTGGTACTTTCCGCGTTCGTGGCGAGGTGATAGATATCTTCCCGGCAGAATCGGATGACATTGCACTTCGCGTGGAAC > minE/520497‑520626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |