| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 526,903 | C→T | 40.7% | L189L (CTG→TTG) | ybhL → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 526,903 | 0 | C | T | 40.7% | 35.4 / 55.4 | 81 | L189L (CTG→TTG) | ybhL | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (29/19); new base T (7/26); total (36/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.09e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.38e-03 | |||||||||||
GATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGG > minE/526836‑526970 | gATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAAc > 1:1744631/1‑68 (MQ=255)gATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAAc > 1:2864460/1‑68 (MQ=255) ggCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGaaaaa < 1:765075/69‑1 (MQ=255) ggCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGaaaaa < 1:2817405/69‑1 (MQ=255) ggCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGaaaaa < 1:2115454/69‑1 (MQ=255) ggCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGaaaaa < 1:2924665/69‑1 (MQ=255) ggCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGaaaaa < 1:94883/69‑1 (MQ=255) ggCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGaaaaa < 1:2689522/69‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1950469/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1767530/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1861131/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:275258/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:716178/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1946423/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:3006320/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:2562419/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:2459988/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:3393211/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:2450352/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:2759885/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1558315/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:804850/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:147781/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1394709/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1308402/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1282643/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1159392/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:2930796/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1140355/68‑1 (MQ=255) cAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1082280/68‑1 (MQ=255) aGTTACCTACATCTGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:1333427/67‑1 (MQ=255) aGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:2322509/67‑1 (MQ=255) aCCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAatat > 1:2856838/1‑65 (MQ=255) aCCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATgg > 1:967635/1‑67 (MQ=255) aCCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATgg > 1:1163332/1‑67 (MQ=255) aCCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATgg > 1:734837/1‑67 (MQ=255) tGTCTTTGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAat < 1:3021773/46‑1 (MQ=255) gTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCcgc < 1:1142792/69‑1 (MQ=255) gTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCcgc < 1:847750/69‑1 (MQ=255) tCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCcgc < 1:3072082/68‑1 (MQ=255) tGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAATATGGGTGAGCAGATTGATAccc > 1:2447382/1‑62 (MQ=255) tGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAATATGGGTGAGCAGATTGATAccc > 1:359912/1‑62 (MQ=255) tGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAATATGGGTGAGCAGATTGATAccc > 1:2062889/1‑62 (MQ=255) tGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAATATGGGTGAGCAGATTGATAccc > 1:3095085/1‑62 (MQ=255) tGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAATATGGGTGAGCAGATTGATAccc > 1:1656880/1‑62 (MQ=255) tGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAATATGGGTGAGCAGATTGATAccc > 1:1496092/1‑62 (MQ=255) tGTCGGATTGACGGCGTATGACACTCAGAAATTGAAAAATATGGGTGAGCAGATTGATAccc > 1:3011769/1‑62 (MQ=255) tGTCGGATGGACGGCGTATGACACTCAGAAATTGAAAAat < 1:3062494/40‑1 (MQ=255) aTTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGaa < 1:3096986/69‑1 (MQ=255) aTTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGaa < 1:1261964/69‑1 (MQ=255) aTTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGaa < 1:2765548/69‑1 (MQ=255) aTTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGaa < 1:1893028/69‑1 (MQ=255) aTTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGa < 1:2615663/68‑1 (MQ=255) ttGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGaa < 1:321709/68‑1 (MQ=255) cGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCa < 1:3372808/69‑1 (MQ=255) cGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCa < 1:2610239/69‑1 (MQ=255) cGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCa < 1:2649658/69‑1 (MQ=255) cGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCa < 1:2599867/69‑1 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCtt > 1:2193616/1‑68 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:2211115/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:1063602/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:1212377/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:1352326/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:1910581/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:1913728/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:597463/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:458727/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:2240731/1‑69 (MQ=255) aaCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:2790937/1‑69 (MQ=255) aaCTGAAAAATAAGGGTGAGCAGATTGATACCcgcg > 1:936057/1‑36 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:3241690/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:3208758/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:3431636/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:2173964/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:3207406/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:3110920/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:2412149/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:1024332/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:1485769/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:2678751/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTgg > 1:2931309/1‑69 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:2938290/1‑68 (MQ=255) aCTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTg > 1:1514647/1‑68 (MQ=255) | GATGTGGGCAGTTACCTACATCGGCGTGATTGTCTTTGTCGGATTGACGGCGTATGACACGCAGAAACTGAAAAATATGGGTGAGCAGATTGATACCCGCGACACGTCGAACCTGCGCAAATATTCCATTCTTGG > minE/526836‑526970 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |