| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,837,204 | T→C | 26.1% | E39G (GAA→GGA) | yjeK ← | predicted lysine aminomutase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,837,204 | 0 | T | C | 26.1% | 78.5 / 31.1 | 51 | E39G (GAA→GGA) | yjeK | predicted lysine aminomutase |
| Reads supporting (aligned to +/- strand): ref base T (13/24); new base C (8/5); total (22/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.16e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
CGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGTT > minE/2837135‑2837272 | cgatcgatAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTtc > 1:367900/1‑71 (MQ=255) atAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCcgc < 1:1861748/71‑1 (MQ=255) tAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCcgcg > 1:2488574/1‑71 (MQ=255) aaTGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTc < 1:1087215/71‑1 (MQ=255) aGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCtata > 1:2171334/1‑71 (MQ=255) cacGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGa > 1:539861/1‑71 (MQ=255) cGCAGGGCAAAAAGCTTTTTGGCGCTGCGTAAGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGAc > 1:1971145/1‑70 (MQ=255) gCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACg > 1:3136112/1‑70 (MQ=255) aGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAg < 1:2837239/71‑1 (MQ=255) aGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAg < 1:1750510/71‑1 (MQ=255) ggCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGAc < 1:3092369/65‑1 (MQ=255) ggCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAg > 1:574489/1‑69 (MQ=255) ggCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGa < 1:594269/70‑1 (MQ=255) gCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGAc > 1:1677525/1‑64 (MQ=255) gCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAg > 1:1266071/1‑71 (MQ=255) cAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGt > 1:4517700/1‑71 (MQ=255) aaaaGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGtt > 1:1348110/1‑70 (MQ=255) aaaaGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGtt > 1:905609/1‑70 (MQ=255) aaaGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtca < 1:726527/71‑1 (MQ=255) aGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCaaaa > 1:1532222/1‑55 (MQ=255) tttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtca < 1:3394777/66‑1 (MQ=255) tttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtca < 1:2567828/66‑1 (MQ=255) tttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtc < 1:2575059/65‑1 (MQ=255) tttttGGCGCTGCGTCCGGCTAACAGTTTTACCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAg > 1:1574120/1‑70 (MQ=255) ttttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGa > 1:4186769/1‑71 (MQ=255) ttttGGCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGa > 1:972868/1‑71 (MQ=255) tttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAgg < 1:611251/69‑1 (MQ=255) tttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGAt > 1:2103004/1‑71 (MQ=255) ttGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGAt < 1:1748698/70‑1 (MQ=255) ttGGCGCTGCGTCAGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGa < 1:3422080/69‑1 (MQ=255) ggCGCTGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAggt > 1:2974499/1‑66 (MQ=255) gcTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTca > 1:708063/1‑71 (MQ=255) cTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac < 1:1309469/71‑1 (MQ=255) cTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac < 1:2286513/71‑1 (MQ=255) tGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCaaaa < 1:2789719/42‑1 (MQ=255) tGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac > 1:2755181/1‑70 (MQ=255) tGCGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac > 1:3805300/1‑70 (MQ=255) cGTCCGGCTAACAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCacaac < 1:3251887/71‑1 (MQ=255) tcaggCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACAt < 1:4224415/68‑1 (MQ=255) tCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCacaaca > 1:1274591/1‑70 (MQ=255) tCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACAt < 1:4343032/71‑1 (MQ=255) cGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCg < 1:3644892/71‑1 (MQ=255) gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGt < 1:3460306/45‑1 (MQ=255) gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTcac < 1:4547469/62‑1 (MQ=255) gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGc < 1:186624/71‑1 (MQ=255) gCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGc < 1:140642/71‑1 (MQ=255) cTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGc < 1:143864/70‑1 (MQ=255) aaCAGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAg < 1:732662/38‑1 (MQ=255) aCAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCaa < 1:1016060/69‑1 (MQ=255) aCAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCaa < 1:2539962/69‑1 (MQ=255) aCAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCaa < 1:2545455/69‑1 (MQ=255) aGTTTTCCCTCCGCGTCTATATTCAAAAGACGCAGAAGt > 1:1916647/1‑39 (MQ=255) tttttCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGc > 1:3748100/1‑70 (MQ=255) tttcCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTca > 1:4165931/1‑52 (MQ=255) tttcCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGt > 1:289099/1‑71 (MQ=255) tttCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGt > 1:536022/1‑70 (MQ=255) ttCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGtt < 1:791879/70‑1 (MQ=255) | CGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTCACAACATCGGCAAGTTGCGTT > minE/2837135‑2837272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |