Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,632,970 A→G 100% K421R (AAA→AGA)  livM → leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,632,9700AG100.0% 187.1 / NA 49K421R (AAA→AGA) livMleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/49);  total (0/49)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCAT  >  minE/2632914‑2632984
                                                        |              
gCAGGGCTTGCTGCCCTTGACGCGCCCGCAACTGAAGCTGAAAATCGTCGCAGCGAGAGGAGAGCAGGCAt  <  1:417971/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:472022/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:358837/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:379681/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:396606/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:406596/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:41890/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:441356/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:458831/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:464534/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:469061/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:109513/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:493864/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:505253/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:524105/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:575845/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:599090/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:612128/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:614359/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:73398/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:98026/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:318431/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:113645/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:133830/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:141287/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:147116/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:14759/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:184581/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:198764/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:213270/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:23318/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:253768/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:256524/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:257765/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:282020/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:284482/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:291341/71‑1 (MQ=255)
gCAGGGCTTGCTGCCCATGACGAGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:349120/71‑1 (MQ=255)
gCAGGGCTTGATGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:525547/71‑1 (MQ=255)
gCAGGGCGTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:485022/71‑1 (MQ=255)
 cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:257518/70‑1 (MQ=255)
 cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:53769/70‑1 (MQ=255)
 cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:554039/70‑1 (MQ=255)
 cAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:382689/70‑1 (MQ=255)
  aGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:255082/69‑1 (MQ=255)
             cccATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:72317/58‑1 (MQ=255)
                   aCGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:10250/52‑1 (MQ=255)
                              aCTGAAGCTGATAATCGGCGCAGCGAGAGGAGAGCAGGctt  <  1:253387/41‑3 (MQ=25)
                                   aGCTGAAAAACGGCGCAGCGAGAGGAGAGCAGGCAt  <  1:512375/36‑1 (MQ=255)
                                                        |              
GCAGGGCTTGCTGCCCATGACGCGCCCGCAACTGAAGCTGAAAAACGGCGCAGCGAAAGGAGAGCAGGCAT  >  minE/2632914‑2632984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑