| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 462,141 | C→A | 100% | L43M (CTG→ATG) | sdhD → | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 462,141 | 0 | C | A | 88.9% | 20.4 / ‑2.0 | 9 | L43M (CTG→ATG) | sdhD | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (0/8); total (0/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGT > minE/462085‑462154 | tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGt < 1:629468/70‑1 (MQ=255)tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:109606/70‑1 (MQ=255)tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:121222/70‑1 (MQ=255)tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:277511/70‑1 (MQ=255)tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:338863/70‑1 (MQ=255)tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:55419/70‑1 (MQ=255)tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:623334/70‑1 (MQ=255)tCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:636313/70‑1 (MQ=255) ccTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGATGACATATGAAGt < 1:270694/66‑1 (MQ=255) | TCGTCCTGACGCTCTACATCATTTATATGGTCGGTTTTTTCGCTACCAGTGGCGAGCTGACATATGAAGT > minE/462085‑462154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |