| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,827,842 | Δ1 bp | coding (1085/2562 nt) | mutS → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,827,840 | 0 | C | . | 98.1% | 211.8 / ‑1.0 | 53 | coding (1083/2562 nt) | mutS | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (23/29); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
ACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGA > minE/1827771‑1827905 | aCAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGc > 1:2887799/1‑70 (MQ=255)aCAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGc > 1:2097595/1‑70 (MQ=255) cAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGc < 1:2943074/69‑1 (MQ=255) cGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCAcgcg < 1:1041886/71‑1 (MQ=255) cGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCAcgcg < 1:1932999/71‑1 (MQ=255) gTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGAt < 1:651444/71‑1 (MQ=255) tACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATc < 1:1225313/71‑1 (MQ=255) aCTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATc < 1:3230/70‑1 (MQ=255) aCTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCt > 1:2781206/1‑71 (MQ=255) cTGCGTCAGGTCGGCGTCCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTg < 1:2773051/71‑1 (MQ=255) cTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTg > 1:716059/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:2575562/1‑71 (MQ=255) tCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:182459/1‑71 (MQ=255) cAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCg > 1:1706150/1‑70 (MQ=255) aGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTa > 1:1546038/1‑71 (MQ=255) aGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTa > 1:32450/1‑71 (MQ=255) aGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTa > 1:974963/1‑71 (MQ=255) ggTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTAt < 1:2567353/71‑1 (MQ=255) cGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCt > 1:1264378/1‑59 (MQ=255) cGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgcg > 1:1918158/1‑71 (MQ=255) cGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgcg > 1:1571352/1‑71 (MQ=255) cGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATgcg > 1:604141/1‑71 (MQ=255) gACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCAc > 1:102930/1‑71 (MQ=255) aCCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACg < 1:730964/71‑1 (MQ=255) catGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGc > 1:2471490/3‑71 (MQ=255) cTGGAACGTATTCTGGCACGTCTGGCTTTACGAGCTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCt < 1:1035297/71‑1 (MQ=255) cTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCt < 1:533173/71‑1 (MQ=255) cTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCt < 1:1880766/71‑1 (MQ=255) gAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTc < 1:2479882/71‑1 (MQ=255) gAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTc > 1:1358133/1‑71 (MQ=255) gAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTc < 1:1117271/71‑1 (MQ=255) aaCGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTc < 1:2838416/70‑1 (MQ=255) aCGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATc > 1:2767782/1‑46 (MQ=255) cGTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCtt > 1:2403979/1‑66 (MQ=255) gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:2606384/71‑1 (MQ=255) gTATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGc < 1:1282672/71‑1 (MQ=255) tATTCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCa < 1:664009/71‑1 (MQ=255) ttCTGGCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCaa < 1:1819741/70‑1 (MQ=255) ggCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATg > 1:1830290/1‑48 (MQ=255) gCACGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGc < 1:2250316/69‑1 (MQ=255) cGTCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAg < 1:239516/71‑1 (MQ=255) tCTGGCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCt < 1:2711377/71‑1 (MQ=255) gCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgt > 1:780706/1‑71 (MQ=255) gCTTTACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgc < 1:3048827/69‑1 (MQ=255) tttACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCAACGCTTTCCAGCAACTGCCGGAGCtgcgtg > 1:775398/1‑70 (MQ=255) ttACGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtg < 1:1971184/69‑1 (MQ=255) aCGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgcg > 1:356316/1‑69 (MQ=255) aCGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgcg > 1:397155/1‑69 (MQ=255) aCGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTgcgc < 1:2512174/70‑1 (MQ=255) cGAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCa < 1:1185564/70‑1 (MQ=255) gAACTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGt < 1:2729936/71‑1 (MQ=255) aaCTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGtt < 1:715991/71‑1 (MQ=255) aCTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCtt < 1:2572966/39‑1 (MQ=38) aCTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTg > 1:1812230/1‑50 (MQ=39) aCTGCTCG‑CCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTa < 1:153865/71‑1 (MQ=255) cTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGa < 1:998564/70‑1 (MQ=255) | ACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGA > minE/1827771‑1827905 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |