Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,953,337	Δ1 bp	100%	coding (772/1326 nt)	pepP ←	proline aminopeptidase P II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,953,337	0	C	.	87.5%	20.9 / ‑1.2	8	coding (772/1326 nt)	pepP	proline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (0/7);  total (0/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGC  >  minE/1953304‑1953380                                  |                                            aaCCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTc            <  1:1410070/67‑1 (MQ=255)           ttCACAACCCGCGTCAATCAACA‑CAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGc  <  1:1292028/66‑1 (MQ=255)           ttCACAACCCGCGTCAATCAACA‑CAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGc  <  1:1340279/66‑1 (MQ=255)           ttCACAACCCGCGTCAATCAACA‑CAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGc  <  1:1425205/66‑1 (MQ=255)           ttCACAACCCGCGTCAATCAACA‑CAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGc  <  1:1546972/66‑1 (MQ=255)           ttCACAACCCGCGTCAATCAACA‑CAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGc  <  1:1850011/66‑1 (MQ=255)           ttCACAACCCGCGTCAATCAACA‑CAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGc  <  1:1868154/66‑1 (MQ=255)           ttCACAACCCGCGTCAATCAACA‑CAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGc  <  1:88870/66‑1 (MQ=255)                                  |                                            AACCTTTGTATTCACAACCCGCGTCAATCAACACCAGGTCGCCGTCGCGCATTTCACACTCGTTTTCGGTGTAGTGC  >  minE/1953304‑1953380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑