Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,148,996	A→G	100%	Y56Y (TAT→TAC)	glmM ←	phosphoglucosamine mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,148,996	0	A	G	98.3%	198.7 / ‑4.4	58	Y56Y (TAT→TAC)	glmM	phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (0/57);  minor base T (0/1);  total (0/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTG  >  minE/2148949‑2149014                                                |                   atcaGGCCCGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:119468/63‑1 (MQ=255)      gCCCGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:788942/61‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCGAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:363484/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATTTAGCCAGAAATACGCgtg  <  1:2029341/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:39928/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:2088351/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:2145434/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:215708/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:2210422/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:2220544/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:2314800/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:236389/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:300074/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:908826/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:857176/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:389581/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:837637/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:4238/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:489389/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:505126/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:517719/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:538837/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:559233/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:610035/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:612571/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:638301/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:812764/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:812998/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:2022204/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1001189/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1033456/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1041689/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1078055/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:110548/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1189253/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1265966/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1279154/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1318588/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1323350/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1537483/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1557549/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1628159/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1766115/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1852235/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1852330/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1905550/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1911026/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1923652/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:193701/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1975086/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1989019/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:2020723/59‑1 (MQ=255)        ccGCAGCCGCCAGACCCGCTTCAAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:1693711/59‑1 (MQ=255)        ccGCAGCCGCCAGACACGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:421779/59‑1 (MQ=255)                   aGACCCGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:951602/48‑1 (MQ=255)                      cccGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:351168/45‑1 (MQ=255)                      cccGCTTCCAGTGCTGACTCCAGCATGTAGCCAGAAATACGCgtg  <  1:345048/45‑1 (MQ=255)                                gTGCTGACTGCAGCATGTAGCCAGAAATACGCgtg  <  1:1030738/35‑1 (MQ=37)                                                |                   GAAAGGCCCGCTGCCGCCAGACCCGCTTCCAGTGCTGACTCCAGCATATAGCCAGAAATACGCGTG  >  minE/2148949‑2149014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑