BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	2,732,305:1	+G	22.9%	coding (375/723 nt)	yheO →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,732,305	1	.	G	22.9%	198.7 / 116.1	105	coding (375/723 nt)	yheO	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base . (40/41); new base G (9/15); total (49/56)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 106 total aligned reads displayed.

AGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTGCCGC  >  minE/2732246‑2732372

aGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctg                                                               >  1:3616878/1‑66 (MQ=255)

aGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctg                                                               >  1:278949/1‑66 (MQ=255)

aGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctg                                                               >  1:2222989/1‑66 (MQ=255)

aGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCtgtg                                                          <  1:3898259/71‑1 (MQ=255)

aGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCtgtg                                                          <  1:1871889/71‑1 (MQ=255)

   ggcgTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCAt                                                       <  1:2684062/71‑1 (MQ=255)

    gcgTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATc                                                      <  1:1262419/71‑1 (MQ=255)

    gcgTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATc                                                      <  1:1611355/71‑1 (MQ=255)

      gTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCTCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa                                                    <  1:2402956/71‑1 (MQ=255)

      gTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa                                                    <  1:1168261/71‑1 (MQ=255)

       tATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCaa                                                    <  1:2803498/71‑1 (MQ=255)

         ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc                                                              <  1:1053407/59‑1 (MQ=39)

         ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc                                                              <  1:1073370/59‑1 (MQ=39)

         ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc                                                              <  1:3552985/59‑1 (MQ=39)

         ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCtgtg                                                          <  1:1031988/62‑1 (MQ=255)

         ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCtgtg                                                          <  1:4053972/62‑1 (MQ=255)

         ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCAt                                                       <  1:50925/65‑1 (MQ=255)

           aaTGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATg                                                >  1:1570796/1‑70 (MQ=255)

           aaTGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATg                                                >  1:1885360/1‑70 (MQ=255)

             tGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGaa                                              <  1:2217366/71‑1 (MQ=255)

                aGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCtt                                          <  1:3072165/71‑1 (MQ=255)

                aGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCtt                                          <  1:177377/71‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCTTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTg                                         <  1:3678411/71‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCa                                                        <  1:3651266/56‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCa                                                        <  1:1655279/56‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGa                                               <  1:1423854/65‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGa                                               <  1:3659640/65‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTg                                         <  1:2003414/71‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTg                                         <  1:2528224/71‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTg                                         <  1:2942981/71‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTg                                         <  1:3255863/71‑1 (MQ=255)

                 gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTg                                         <  1:2718165/71‑1 (MQ=255)

                  tCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCtt                                          <  1:3100865/69‑1 (MQ=255)

                  tCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCtt                                          <  1:1673762/69‑1 (MQ=255)

                  tCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCtt                                          <  1:2804593/69‑1 (MQ=255)

                  tCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCtt                                          <  1:2758071/69‑1 (MQ=255)

                   cccTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGa                                        >  1:1736312/1‑71 (MQ=255)

                    ccTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGa                                        <  1:2623956/70‑1 (MQ=255)

                    ccTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGa                                        >  1:784303/1‑69 (MQ=255)

                    ccTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGa                                        >  1:3815835/1‑69 (MQ=255)

                      tGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCaa                                                    <  1:2954368/56‑1 (MQ=39)

                         cTATCGCGATTCGTAAGCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTccc                                 >  1:2488124/1‑71 (MQ=255)

                          tATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctgctg                                                            <  1:2052409/43‑1 (MQ=255)

                          tATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctgctg                                                            <  1:2704765/43‑1 (MQ=255)

                            tCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtc                              >  1:1409211/1‑71 (MQ=255)

                            tCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtc                              >  1:2985816/1‑71 (MQ=255)

                             cgcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtc                              >  1:539111/1‑71 (MQ=255)

                              gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTg                                         <  1:4008225/59‑1 (MQ=255)

                              gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAat                                                   >  1:669213/1‑48 (MQ=255)

                              gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAat                                                   >  1:2613531/1‑48 (MQ=255)

                              gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAat                                                   >  1:3084530/1‑48 (MQ=255)

                              gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAat                                                   >  1:2092435/1‑48 (MQ=255)

                              gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAat                                                   >  1:204972/1‑48 (MQ=255)

                              gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtctc                            >  1:3351806/1‑71 (MQ=255)

                                   tCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtct                             >  1:669547/1‑66 (MQ=255)

                                   tCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtct                             >  1:884714/1‑66 (MQ=255)

                                   tCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtct                             >  1:904183/1‑66 (MQ=255)

                                   tCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCg                           >  1:3014668/1‑67 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       <  1:2897105/70‑1 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       <  1:1878169/70‑1 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       <  1:2988673/70‑1 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       <  1:1099610/70‑1 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt                      >  1:2922481/1‑71 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt                      >  1:3388472/1‑71 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt                      >  1:2185152/1‑71 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt                      >  1:1698444/1‑71 (MQ=255)

                                    cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt                      >  1:2324079/1‑71 (MQ=255)

                                        aCCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTAt                   <  1:75363/71‑1 (MQ=255)

                                        aCCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTAt                   <  1:2057217/70‑1 (MQ=255)

                                        aCCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCCCGCAGATTAt                   >  1:2557219/1‑70 (MQ=255)

                                          cgcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGa                                               >  1:1284360/1‑40 (MQ=255)

                                          cgcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGa                                               >  1:1348121/1‑40 (MQ=255)

                                          cgcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGa                                               >  1:2361902/1‑40 (MQ=255)

                                          cgcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGa                                               >  1:430915/1‑40 (MQ=255)

                                           gcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAg                >  1:3460725/1‑70 (MQ=255)

                                           gcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGc               <  1:630740/71‑1 (MQ=255)

                                                cAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCt           <  1:104712/71‑1 (MQ=255)

                                                 aGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCtt          >  1:66774/1‑70 (MQ=255)

                                                   cGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTAt                   <  1:3778688/60‑1 (MQ=255)

                                                   cGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTAt                   <  1:2499413/60‑1 (MQ=255)

                                                   cGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTATCGCAGATTAt                   <  1:3648655/60‑1 (MQ=255)

                                                     tgtAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGa                 >  1:3672722/1‑60 (MQ=255)

                                                     tgtAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGa                 >  1:689185/1‑60 (MQ=255)

                                                     tgtAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGa                 >  1:1481555/1‑60 (MQ=255)

                                                     tgtAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGa                 >  1:1566172/1‑60 (MQ=255)

                                                     tgtAATT‑GGTCTGCTGTGCATCAATATGAGTCTTGATGt                                     <  1:421147/39‑1 (MQ=255)

                                                     tgtAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGt                                     <  1:1244602/39‑1 (MQ=255)

                                                     tgtAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGt                                     <  1:1623318/39‑1 (MQ=255)

                                                     tgtAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGt                                     <  1:3062700/39‑1 (MQ=255)

                                                     tgtAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGt                                     <  1:3432901/39‑1 (MQ=255)

                                                       tAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGc               <  1:1183962/60‑1 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATTTGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTgccgc  >  1:1198040/1‑70 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       >  1:1186189/1‑49 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       >  1:910980/1‑49 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       >  1:2155925/1‑49 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       >  1:2160779/1‑49 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGa                       >  1:2451525/1‑49 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTAt                   >  1:3228812/1‑53 (MQ=255)

                                                         aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTgccgc  >  1:1441129/1‑70 (MQ=255)

                                                          tt‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTgccgc  >  1:436110/1‑69 (MQ=255)

AGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTGCCGC  >  minE/2732246‑2732372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`