| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,732,305:1 | +G | 40.4% | coding (375/723 nt) | yheO → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,732,305 | 1 | . | G | 40.4% | ‑4.6 / 213.5 | 94 | coding (375/723 nt) | yheO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base . (28/28); new base G (12/26); total (40/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.16e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 101 total aligned reads displayed.
CAAAAGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTGCC > minE/2732242‑2732370 | cAAAAGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctgc < 1:1736123/71‑1 (MQ=255)cAAAAGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctgc < 1:2177251/71‑1 (MQ=255)cAAAAGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctgc < 1:2359767/71‑1 (MQ=255)cAAAAGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctgc < 1:251267/71‑1 (MQ=255)cAAAAGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTctgc < 1:3552073/71‑1 (MQ=255) aaaaGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc > 1:1018707/1‑71 (MQ=255) aaaGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAAtt > 1:2472001/1‑62 (MQ=255) aaaGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAAtt > 1:2765272/1‑62 (MQ=255) aaaGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAAtt > 1:935935/1‑62 (MQ=255) gTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:1333228/71‑1 (MQ=255) gTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:1800759/71‑1 (MQ=255) aTTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCAt < 1:2105032/66‑1 (MQ=255) aTTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCAt < 1:2093422/66‑1 (MQ=255) aTTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCAt < 1:2845758/66‑1 (MQ=255) ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc < 1:1143926/59‑1 (MQ=39) ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc < 1:258993/59‑1 (MQ=39) ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAata > 1:1574106/1‑71 (MQ=255) ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑gg > 1:737913/1‑53 (MQ=255) ttAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑gg > 1:2953810/1‑53 (MQ=255) aaTGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATg > 1:1004231/1‑70 (MQ=255) aaTGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATg > 1:1342922/1‑70 (MQ=255) aaTGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATg > 1:3648428/1‑70 (MQ=255) aTGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATg < 1:1093120/70‑1 (MQ=255) tGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGt > 1:1072174/1‑50 (MQ=255) tGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:3842145/64‑1 (MQ=255) tGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:2101245/64‑1 (MQ=255) tGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:3573616/64‑1 (MQ=255) tGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:1863834/64‑1 (MQ=255) tGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:1697183/64‑1 (MQ=255) aGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCt > 1:2075082/1‑71 (MQ=255) aGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCt > 1:696982/1‑71 (MQ=255) aGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCt > 1:721060/1‑71 (MQ=255) gTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCtt < 1:1571625/71‑1 (MQ=255) aCTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc < 1:1967503/44‑1 (MQ=38) aCTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc < 1:2034493/44‑1 (MQ=38) aCTATCGCGATTCGTAACCGCGAACAGCGTGTAATTGGGTctgc < 1:3028731/44‑1 (MQ=38) aCTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGa > 1:3134895/1‑58 (MQ=255) aCTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTcc < 1:964103/71‑1 (MQ=255) tATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCtgtg > 1:4009426/1‑45 (MQ=255) tATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCtgtg > 1:878990/1‑45 (MQ=255) tATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATc < 1:535299/49‑1 (MQ=255) tCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtc > 1:3776414/1‑71 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATc > 1:3926808/1‑56 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATc > 1:2119771/1‑56 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATc > 1:3636297/1‑56 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATc > 1:3231046/1‑56 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATc > 1:4147914/1‑56 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGtt < 1:1844952/64‑1 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtct > 1:2805539/1‑71 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCt > 1:1942959/1‑56 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCt > 1:3692190/1‑56 (MQ=255) gcgATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTtctc > 1:4090793/1‑71 (MQ=255) aTTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCtt < 1:1662652/55‑1 (MQ=255) aTTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCtt < 1:293809/55‑1 (MQ=255) aTTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCtt < 1:1089928/55‑1 (MQ=255) aTTCGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCtt < 1:1334309/55‑1 (MQ=255) ttCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCtt < 1:3218837/53‑1 (MQ=255) cGTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCg > 1:786270/1‑67 (MQ=255) cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt > 1:3863758/1‑71 (MQ=255) cGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt > 1:3618277/1‑71 (MQ=255) gTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:2225082/71‑1 (MQ=255) gTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:717688/71‑1 (MQ=255) gTAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:2114203/71‑1 (MQ=255) gTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCaa < 1:1879665/40‑1 (MQ=255) gTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAtt < 1:2551081/71‑1 (MQ=255) gTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:841027/70‑1 (MQ=255) gTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:313865/70‑1 (MQ=255) tAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAtt < 1:3400289/71‑1 (MQ=255) tAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAtt < 1:751634/71‑1 (MQ=255) tAACCGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAtt < 1:2035379/71‑1 (MQ=255) tAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:2724493/69‑1 (MQ=255) aCCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGa > 1:4052202/1‑49 (MQ=255) aCCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTAt < 1:1513053/70‑1 (MQ=255) ccGCGAACAGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATg < 1:4089867/71‑1 (MQ=255) gcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGc < 1:2282447/71‑1 (MQ=255) gcgAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGc < 1:193099/71‑1 (MQ=255) gcgAACAGCGTGTAATT‑‑GTCTGCTGTGCATCAATATGAATCTTGATGTTcc > 1:688445/1‑51 (MQ=255) aGCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCtt < 1:1032078/71‑1 (MQ=255) gCGTGTAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCttt < 1:2307204/71‑1 (MQ=255) gCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTtg > 1:1500453/1‑71 (MQ=255) gCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTtg > 1:216027/1‑71 (MQ=255) gCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTtg > 1:2123227/1‑71 (MQ=255) gCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTtg > 1:17111/1‑71 (MQ=255) gCGTGTAATT‑GGGCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:1184392/57‑1 (MQ=255) tgtAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAt < 1:3099070/55‑1 (MQ=255) tgtAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTtgtg < 1:310658/71‑1 (MQ=255) tgtAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTACCTTCTCGCatat < 1:3863098/55‑1 (MQ=255) tgtAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCa > 1:2650002/1‑62 (MQ=255) tgtAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCa > 1:626827/1‑62 (MQ=255) gtAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTgcc > 1:2909601/1‑71 (MQ=255) tAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTtgt < 1:2451025/68‑1 (MQ=255) tAATTGGGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTtgt < 1:4084858/68‑1 (MQ=255) aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCa > 1:1989054/1‑47 (MQ=255) aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCa > 1:519146/1‑47 (MQ=255) aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCa > 1:749759/1‑47 (MQ=255) aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCa > 1:2514090/1‑47 (MQ=255) aTT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCa > 1:2074808/1‑47 (MQ=255) aTT‑GGTATGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCa > 1:499/1‑47 (MQ=255) tt‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGAtt < 1:3100981/50‑1 (MQ=255) tt‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTAGCAGAtt < 1:1223297/50‑1 (MQ=255) | CAAAAGCGGCGTATTAATGAAGTCCCTGACTATCGCGATTCGTAACCGCGAACAGCGTGTAATT‑GGTCTGCTGTGCATCAATATGAATCTTGATGTTCCCTTCTCGCAGATTATGAGCACCTTTGTGCC > minE/2732242‑2732370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |