| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,019:1 | +GC | 100% | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,019 | 1 | . | G | 97.9% | 159.6 / ‑3.4 | 47 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (21/25); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 1,892,019 | 2 | . | C | 97.9% | 158.6 / ‑3.7 | 47 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (21/25); total (22/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACC > minE/1891951‑1892078 || cTGGCTTTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑Cag < 1:3162540/70‑2 (MQ=255) tGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAAc > 1:3022377/1‑71 (MQ=255) tGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAAc > 1:3547098/1‑71 (MQ=255) gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAAcc > 1:135972/1‑71 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAAcc < 1:876678/70‑1 (MQ=255) aaaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCg > 1:336944/1‑71 (MQ=255) aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCgg < 1:3582697/71‑1 (MQ=255) aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCgg > 1:3092738/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCggg > 1:4124870/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCggg > 1:1467700/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCggg > 1:2025014/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCggg > 1:3404617/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCggg > 1:1203488/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCgg < 1:3750739/70‑1 (MQ=255) gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATa < 1:1502913/71‑1 (MQ=255) gattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATa < 1:1775677/71‑1 (MQ=255) attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATa < 1:873904/70‑1 (MQ=255) cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGa < 1:387865/70‑1 (MQ=255) cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGa < 1:1152587/70‑1 (MQ=255) aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGa < 1:717044/53‑1 (MQ=255) aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGa < 1:3512737/53‑1 (MQ=255) aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGa < 1:2647261/53‑1 (MQ=255) aGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGa < 1:2845215/53‑1 (MQ=255) aGCACCCCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGa < 1:2282030/53‑1 (MQ=255) cACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGtt > 1:36675/1‑71 (MQ=255) ccGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGaa > 1:1064891/1‑50 (MQ=255) ccGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGaa > 1:2592759/1‑50 (MQ=255) gCTAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg > 1:566275/1‑71 (MQ=255) tAAAACGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt < 1:3734112/70‑1 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATAGC‑‑TAAGCACAGAACCg > 1:1243749/1‑47 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCg > 1:467656/1‑48 (MQ=255) aaaCGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGTATAAGACGTTCGCGtc < 1:1413482/69‑1 (MQ=255) aaCGACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc < 1:3072304/68‑1 (MQ=255) gACATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCgg < 1:1357234/49‑1 (MQ=255) cATTTACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCg < 1:2517524/70‑1 (MQ=255) tttACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATaa < 1:2044171/49‑1 (MQ=255) tttACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATaa < 1:1816954/49‑1 (MQ=255) tttACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATaa < 1:4079426/49‑1 (MQ=255) tttACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATaa < 1:3086344/49‑1 (MQ=255) tACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATaa > 1:1440787/1‑47 (MQ=255) tACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATaa > 1:2562933/1‑47 (MQ=255) tACCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATaa > 1:2063704/1‑47 (MQ=255) aCCGCTCGCTGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTg < 1:2150612/70‑1 (MQ=255) cgctcgctGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc < 1:2637422/53‑1 (MQ=255) cgctcgctGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGAtt > 1:3970011/1‑71 (MQ=255) cgctcgctGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTAGCGtct > 1:2518707/1‑57 (MQ=255) gctGAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt < 1:1240862/70‑1 (MQ=38) gAACATAT‑CAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAcc > 1:2494156/1‑71 (MQ=255) || CTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATAT‑CA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACC > minE/1891951‑1892078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |