Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 641,942 C→A 11.6% A211E (GCG→GAG)  ybdR → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb641,9420CA11.6% 172.6 / 6.1 60A211E (GCG→GAG) ybdRpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (18/34);  new base A (3/4);  total (21/39)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

AAGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGA  >  W3110S.gb/641874‑642010
                                                                    |                                                                     
aaGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCgg                                                                     <  1:2544849/71‑1 (MQ=255)
       gCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑t                                                             >  1:3352780/1‑71 (MQ=255)
           tCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑ttttt                                                         <  1:1983004/71‑1 (MQ=255)
            cTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttg                                                        >  1:1374609/1‑71 (MQ=255)
             tATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                       >  1:3120290/1‑71 (MQ=255)
              aTGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACa                                                                 <  1:1511969/61‑1 (MQ=255)
               tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     <  1:870607/71‑1 (MQ=255)
               tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     >  1:3620783/1‑71 (MQ=255)
               tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     <  1:1310584/71‑1 (MQ=255)
               tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     <  1:1398869/71‑1 (MQ=255)
               tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgtt                                                      <  1:2180130/70‑1 (MQ=255)
               tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                       <  1:363215/69‑1 (MQ=255)
               tGGTGCTGGTCCTGTGGGATTGTCGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     <  1:668107/71‑1 (MQ=255)
                 gTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgt                                                    <  1:2686365/70‑1 (MQ=255)
                 gTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgt                                                    <  1:3364558/70‑1 (MQ=255)
                  tGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑ttt                                                           <  1:2429010/62‑1 (MQ=255)
                  tGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgttg                                                  <  1:20677/71‑1 (MQ=255)
                    cTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     <  1:2666852/66‑1 (MQ=255)
                    cTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGat                                                >  1:3937377/1‑71 (MQ=255)
                    cTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGat                                                <  1:1804336/71‑1 (MQ=255)
                       gTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                       <  1:4075836/61‑1 (MQ=255)
                       gTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatca                                              <  1:1678242/70‑1 (MQ=255)
                            gtgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     >  1:4151925/1‑58 (MQ=255)
                             tgGGATTGTTGACAATCGCCTGTGGACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                       >  1:4285390/1‑71 (MQ=255)
                             tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatca                                              <  1:1709117/64‑1 (MQ=255)
                                    gttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAg                                                                <  1:2911740/40‑1 (MQ=255)
                                    gttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAg                                                                <  1:2677689/40‑1 (MQ=255)
                                       gACAATCGCCTGTGCACGGTTGCTCGGTGCGTAACAGA‑TTTTTGTTGTTGATCATCATCACTACCGCTTg                              >  1:2946617/1‑70 (MQ=255)
                                       gACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTg                              >  1:2097590/1‑70 (MQ=255)
                                        aCAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGc                             <  1:4128438/70‑1 (MQ=255)
                                           aTCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     <  1:395701/43‑1 (MQ=255)
                                           aTCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCAttt                         >  1:2963020/1‑71 (MQ=255)
                                            tCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgttg                                                  <  1:1198890/45‑1 (MQ=255)
                                             cGCCTGTGCACGGTTGCTCGGTGGGGAACAGA‑ttt                                                           <  1:259675/35‑1 (MQ=255)
                                               ccTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                     >  1:185518/1‑39 (MQ=255)
                                                cTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                       <  1:1503491/52‑1 (MQ=255)
                                                 tgtgCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgttg                                                  <  1:713989/40‑1 (MQ=255)
                                                   tgCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATcc                                        >  1:2504747/1‑48 (MQ=255)
                                                   tgCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCgccgcc                  <  1:2865245/70‑1 (MQ=255)
                                                   tgCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGccgccg                 <  1:1262185/71‑1 (MQ=255)
                                                     cACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGAc               >  1:3774264/1‑71 (MQ=255)
                                                        ggTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGc            <  1:1839686/71‑1 (MQ=255)
                                                         gTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATTATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCt           <  1:185090/71‑1 (MQ=255)
                                                          ttGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTTCATTTCGCCGCCGACCGCTa          <  1:2549581/71‑1 (MQ=255)
                                                           tGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTa          <  1:1915810/70‑1 (MQ=255)
                                                            gCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTAc         <  1:4113138/70‑1 (MQ=255)
                                                            gCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACg        >  1:130881/1‑71 (MQ=255)
                                                             aacggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaag                                >  1:2349577‑M1/3‑30 (MQ=255)
                                                               cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttga                                  <  1:1354047‑M1/43‑16 (MQ=255)
                                                               cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttg                                   <  1:1861547‑M1/42‑15 (MQ=255)
                                                               cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttg                                   <  1:2069484‑M1/42‑15 (MQ=255)
                                                                ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc    >  1:545918/1‑71 (MQ=255)
                                                                ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc    <  1:2332878/71‑1 (MQ=255)
                                                                ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaa                                 >  1:2135925‑M1/1‑27 (MQ=255)
                                                                ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaa                                 >  1:3912935‑M1/1‑27 (MQ=255)
                                                                ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttg                                   <  1:2153421‑M1/41‑15 (MQ=255)
                                                                 gTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGcgcg   <  1:2490595/71‑1 (MQ=255)
                                                                  tGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGa  >  1:2774172/1‑71 (MQ=255)
                                                                  tGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGa  >  1:4301808/1‑71 (MQ=255)
                                                                  tGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGa  >  1:2441549/1‑71 (MQ=255)
                                                                    |                                                                     
AAGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGA  >  W3110S.gb/641874‑642010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑