Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 641,942 C→A 15.9% A211E (GCG→GAG)  ybdR → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb641,9420CA15.9% 164.0 / 11.5 57A211E (GCG→GAG) ybdRpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (30/17);  new base A (5/4);  total (35/22)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

TCAAGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGC  >  W3110S.gb/641872‑642008
                                                                      |                                                                   
tCAAGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGc                                                                     >  1:1790707/1‑71 (MQ=255)
tCAAGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGc                                                                     >  1:1817906/1‑71 (MQ=255)
 cAAGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCg                                                                    >  1:3463050/1‑71 (MQ=255)
      gTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                 >  1:2910169/1‑69 (MQ=255)
      gTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                 >  1:1754737/1‑69 (MQ=255)
       ttGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                 >  1:343206/1‑68 (MQ=255)
        tGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                 >  1:1516441/1‑67 (MQ=255)
           aGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑ttt                                                         <  1:2870244/71‑1 (MQ=255)
             tCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑ttttt                                                       <  1:3820584/71‑1 (MQ=255)
                 tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGGGGAACAGA‑TTTttgtt                                                    <  1:3385784/70‑1 (MQ=255)
                 tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                   >  1:897358/1‑71 (MQ=255)
                 tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgtt                                                    <  1:1921184/70‑1 (MQ=255)
                 tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                     <  1:3474186/69‑1 (MQ=255)
                 tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                     <  1:451670/69‑1 (MQ=255)
                  ggTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                   >  1:1912395/1‑70 (MQ=255)
                   gTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgtt                                                 >  1:626174/1‑71 (MQ=255)
                    tGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgttg                                                <  1:1535506/71‑1 (MQ=255)
                      cTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGa                                               <  1:756250/70‑1 (MQ=255)
                          tCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcat                                           <  1:3754596/70‑1 (MQ=255)
                            cTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatcat                                        >  1:2500280/1‑71 (MQ=255)
                            cTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatcat                                        >  1:4201683/1‑71 (MQ=255)
                            cTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                         <  1:2658651/70‑1 (MQ=255)
                               tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                     >  1:3839338/1‑71 (MQ=255)
                               tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                     >  1:3843198/1‑71 (MQ=255)
                               tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                     <  1:3418942/71‑1 (MQ=255)
                                 ggATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTa                                   >  1:2801433/1‑71 (MQ=255)
                                    ttgttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                         >  1:4458377/1‑62 (MQ=255)
                                    ttgttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTAcc                                 >  1:4536371/1‑70 (MQ=255)
                                         gACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                         >  1:1919767/1‑57 (MQ=255)
                                         gACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTg                            <  1:1101156/70‑1 (MQ=255)
                                         gACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTg                            >  1:1386299/1‑70 (MQ=255)
                                          aCAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                     >  1:3730720/1‑44 (MQ=255)
                                             aTCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCAtt                        <  1:1886001/70‑1 (MQ=255)
                                              tCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                         >  1:2031755/1‑52 (MQ=255)
                                              tCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatc                                             <  1:2611710/48‑1 (MQ=255)
                                              tCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTc                      >  1:783596/1‑71 (MQ=255)
                                               cGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatcat                                        >  1:3310906/1‑52 (MQ=255)
                                               cGCCTGTGCACGGTTGATCGGTGCGGAACAGA‑TTTttgtt                                                    >  1:4302969/1‑40 (MQ=255)
                                               cGCCTGTGCACGGTTGATCGGTGCGGAACAGA‑TTTttgtt                                                    >  1:4297649/1‑40 (MQ=255)
                                                gCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGat                                              >  1:4344974/1‑45 (MQ=255)
                                                gCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTcgc                    >  1:713036/1‑71 (MQ=255)
                                                gCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTcg                     <  1:2543904/70‑1 (MQ=255)
                                                    gtgCACGGGTGCTCGGTGCGGAACAGA‑TTTttgtt                                                    <  1:3977965/35‑1 (MQ=255)
                                                     tgCACGTTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                         >  1:10401/1‑45 (MQ=255)
                                                        aCGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatc                                             <  1:284614/38‑1 (MQ=255)
                                                         cGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCa                          >  1:2677630/1‑56 (MQ=255)
                                                               aacggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaag                              >  1:2401107‑M1/3‑30 (MQ=255)
                                                               aacggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaag                              >  1:2953948‑M1/3‑30 (MQ=255)
                                                                acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggtg                           >  1:3595499‑M1/2‑29 (MQ=255)
                                                                acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggt                            <  1:2071070‑M1/47‑20 (MQ=255)
                                                                acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggt                            <  1:2892136‑M1/47‑20 (MQ=255)
                                                                acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaa                               >  1:3358412‑M1/2‑29 (MQ=255)
                                                                 cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggtgta                         <  1:3406317‑M1/50‑23 (MQ=255)
                                                                 cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgtttttt                                  <  1:4227811‑M1/41‑14 (MQ=255)
                                                                  ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTAc                                  >  1:1187128/1‑39 (MQ=255)
                                                                  ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc  <  1:393037/71‑1 (MQ=255)
                                                                  ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc  >  1:1755502/1‑71 (MQ=255)
                                                                  ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc  >  1:341259/1‑71 (MQ=255)
                                                                  ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaagg                             >  1:1833287‑M1/1‑27 (MQ=255)
                                                                      |                                                                   
TCAAGCGTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGC  >  W3110S.gb/641872‑642008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑