Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 641,944 G→A 16.1% E212K (GAA→AAA)  ybdR → predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb641,9440GA16.1% 138.5 / 16.6 56E212K (GAA→AAA) ybdRpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base G (29/18);  new base A (5/4);  total (34/22)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGATCC  >  W3110S.gb/641878‑642013
                                                                  |                                                                      
gTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                      >  1:2910169/1‑69 (MQ=255)
gTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                      >  1:1754737/1‑69 (MQ=255)
 ttGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                      >  1:343206/1‑68 (MQ=255)
  tGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGaa                                                                      >  1:1516441/1‑67 (MQ=255)
     aGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑ttt                                                              <  1:2870244/71‑1 (MQ=255)
       tCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑ttttt                                                            <  1:3820584/71‑1 (MQ=255)
           tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGGGGAACAGA‑TTTttgtt                                                         <  1:3385784/70‑1 (MQ=255)
           tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                        >  1:897358/1‑71 (MQ=255)
           tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgtt                                                         <  1:1921184/70‑1 (MQ=255)
           tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                          <  1:3474186/69‑1 (MQ=255)
           tGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                          <  1:451670/69‑1 (MQ=255)
            ggTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttg                                                        >  1:1912395/1‑70 (MQ=255)
             gTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgtt                                                      >  1:626174/1‑71 (MQ=255)
              tGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgttgttg                                                     <  1:1535506/71‑1 (MQ=255)
                cTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGa                                                    <  1:756250/70‑1 (MQ=255)
                    tCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcat                                                <  1:3754596/70‑1 (MQ=255)
                      cTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatcat                                             >  1:2500280/1‑71 (MQ=255)
                      cTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatcat                                             >  1:4201683/1‑71 (MQ=255)
                      cTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                              <  1:2658651/70‑1 (MQ=255)
                         tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                          >  1:3839338/1‑71 (MQ=255)
                         tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                          >  1:3843198/1‑71 (MQ=255)
                         tgGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATccc                                          <  1:3418942/71‑1 (MQ=255)
                           ggATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTa                                        >  1:2801433/1‑71 (MQ=255)
                              ttgttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                              >  1:4458377/1‑62 (MQ=255)
                              ttgttgACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTAcc                                      >  1:4536371/1‑70 (MQ=255)
                                   gACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                              >  1:1919767/1‑57 (MQ=255)
                                   gACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTg                                 <  1:1101156/70‑1 (MQ=255)
                                   gACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTg                                 >  1:1386299/1‑70 (MQ=255)
                                    aCAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTttgt                                                          >  1:3730720/1‑44 (MQ=255)
                                       aTCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCAtt                             <  1:1886001/70‑1 (MQ=255)
                                        tCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                              >  1:2031755/1‑52 (MQ=255)
                                        tCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatc                                                  <  1:2611710/48‑1 (MQ=255)
                                        tCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTc                           >  1:783596/1‑71 (MQ=255)
                                         cGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatcat                                             >  1:3310906/1‑52 (MQ=255)
                                         cGCCTGTGCACGGTTGATCGGTGCGGAACAGA‑TTTttgtt                                                         >  1:4302969/1‑40 (MQ=255)
                                         cGCCTGTGCACGGTTGATCGGTGCGGAACAGA‑TTTttgtt                                                         >  1:4297649/1‑40 (MQ=255)
                                          gCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGat                                                   >  1:4344974/1‑45 (MQ=255)
                                          gCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTcgc                         >  1:713036/1‑71 (MQ=255)
                                          gCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTcg                          <  1:2543904/70‑1 (MQ=255)
                                              gtgCACGGGTGCTCGGTGCGGAACAGA‑TTTttgtt                                                         <  1:3977965/35‑1 (MQ=255)
                                               tgCACGTTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatcatca                                              >  1:10401/1‑45 (MQ=255)
                                                  aCGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGatc                                                  <  1:284614/38‑1 (MQ=255)
                                                   cGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCa                               >  1:2677630/1‑56 (MQ=255)
                                                         aacggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaag                                   >  1:2401107‑M1/3‑30 (MQ=255)
                                                         aacggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaag                                   >  1:2953948‑M1/3‑30 (MQ=255)
                                                          acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggtg                                >  1:3595499‑M1/2‑29 (MQ=255)
                                                          acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggt                                 <  1:2071070‑M1/47‑20 (MQ=255)
                                                          acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggt                                 <  1:2892136‑M1/47‑20 (MQ=255)
                                                          acgGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaa                                    >  1:3358412‑M1/2‑29 (MQ=255)
                                                           cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaaggtgta                              <  1:3406317‑M1/50‑23 (MQ=255)
                                                           cGGTGAGAAACAGATTTTTTGTTGTTGAagaactgtttttt                                       <  1:4227811‑M1/41‑14 (MQ=255)
                                                            ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTAc                                       >  1:1187128/1‑39 (MQ=255)
                                                            ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc       <  1:393037/71‑1 (MQ=255)
                                                            ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc       >  1:341259/1‑71 (MQ=255)
                                                            ggTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGgcgc       >  1:1755502/1‑71 (MQ=255)
                                                            ggTGAGAAACAGATTTTTTGTTGTTGAagaactgttttttgaagg                                  >  1:1833287‑M1/1‑27 (MQ=255)
                                                                 ggAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGATcc  >  1:2587726/1‑71 (MQ=255)
                                                                  |                                                                      
GTTGCAGTCTATGGTGCTGGTCCTGTGGGATTGTTGACAATCGCCTGTGCACGGTTGCTCGGTGCGGAACAGA‑TTTTTGTTGTTGATCATCATCCCTACCGCTTGCATTTCGCCGCCGACCGCTACGGCGCGATCC  >  W3110S.gb/641878‑642013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑