| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,434,349 | T→A | 40.9% | intergenic (+112/‑197) | fklB → / → cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,434,349 | 0 | T | A | 40.9% | 41.4 / 80.6 | 66 | intergenic (+112/‑197) | fklB/cycA | FKBP‑type peptidyl‑prolyl cis‑trans isomerase/D‑alanine/D‑serine/glycine transporter |
| Reads supporting (aligned to +/- strand): ref base T (20/19); new base A (16/11); total (36/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCT > W3110S.gb/4434281‑4434417 | cAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTAtttt < 1:637142/70‑1 (MQ=255) aaTAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTtgt > 1:146923/1‑69 (MQ=255) aTAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTtgtg > 1:1863760/1‑69 (MQ=255) aTAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTtgtg > 1:1784681/1‑69 (MQ=255) tAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTtgtg < 1:4192346/68‑1 (MQ=255) tAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGc < 1:2464377/71‑1 (MQ=255) cccTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGtt > 1:4535418/1‑71 (MQ=255) cAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTcgcg > 1:1793936/1‑71 (MQ=255) cAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTcg < 1:2588855/69‑1 (MQ=255) aGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTcgcg < 1:4331295/70‑1 (MQ=255) gTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:4178063/71‑1 (MQ=255) tttGCCAAACGGCGCTATTTTGTGTTGCAAAGACC‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:3209798/71‑1 (MQ=255) tGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTtg > 1:1573160/1‑52 (MQ=255) gCCAAACGGCGCTATTTTGTGTTGCAAAGACC‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTAt < 1:2284368/70‑1 (MQ=255) gCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATc < 1:3513384/70‑1 (MQ=255) aaCGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGt < 1:3213866/71‑1 (MQ=255) aaCGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCAcc > 1:4230630/1‑69 (MQ=255) aaCGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCg > 1:2803287/1‑70 (MQ=255) aaCGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGt < 1:1516472/71‑1 (MQ=255) aCGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTg < 1:4412172/71‑1 (MQ=255) aCGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCAc < 1:606181/67‑1 (MQ=255) ggCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTAttttt > 1:4424758/1‑43 (MQ=255) cgcTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:3330204/71‑1 (MQ=255) gcTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGatat < 1:2853945/70‑1 (MQ=255) cTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGa < 1:619261/71‑1 (MQ=255) aTTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATtg > 1:1894304/1‑38 (MQ=255) aTTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTcg > 1:1352934/1‑50 (MQ=255) ttttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTg < 1:4795263/44‑1 (MQ=255) ttttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGacac > 1:3635062/1‑71 (MQ=255) ttttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGtt < 1:4109109/53‑1 (MQ=255) ttttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGt > 1:3452131/1‑52 (MQ=255) ttttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGt > 1:3097042/1‑52 (MQ=255) ttttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTAt > 1:548497/1‑55 (MQ=255) ttttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGacac > 1:251960/1‑71 (MQ=255) tttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:4330823/1‑71 (MQ=255) tttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTATTGTGAGCTg > 1:3219152/1‑43 (MQ=255) ttGTGTTGCAAAGA‑CTCCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:4357217/1‑71 (MQ=255) ttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGa > 1:2910569/1‑66 (MQ=255) ttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGa > 1:2904758/1‑66 (MQ=255) ttGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcact > 1:3967624/1‑70 (MQ=255) tGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACttt > 1:823897/1‑70 (MQ=255) gCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTa < 1:3648060/70‑1 (MQ=255) gCAAAGA‑C‑CCAGTAAGAGTGTATTTTTGTGAGCTTTTTCGCGTTATCACCGTGatat < 1:595538/57‑1 (MQ=255) cAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTaaa > 1:3982237/1‑71 (MQ=255) c‑cccGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTAt < 1:707617/39‑1 (MQ=255) c‑cccGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTAt < 1:2155268/39‑1 (MQ=255) cccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaaaa > 1:982532/1‑71 (MQ=255) ccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:35904/35‑1 (MQ=255) ccGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGtt < 1:4405150/35‑1 (MQ=255) cGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAt > 1:1806911/1‑70 (MQ=255) cGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTa < 1:3948552/35‑1 (MQ=255) cGTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGAcactc > 1:455203/1‑54 (MQ=255) gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCa > 1:283677/1‑37 (MQ=255) gTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCAc > 1:3259120/1‑38 (MQ=255) gTAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAAtt > 1:4280798/1‑70 (MQ=255) tAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACa < 1:1826626/62‑1 (MQ=255) tAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTaa > 1:1680523/1‑71 (MQ=255) tAAGCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGt < 1:696512/40‑1 (MQ=255) aGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAAc < 1:4142510/70‑1 (MQ=255) gCGTGTATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt < 1:644850/71‑1 (MQ=255) gtgtATTATTGTGAGCTGTTTCGCGTTATCACCGTGa > 1:1836701/1‑37 (MQ=255) gtgtATTATTGTGAGCTGTTTCGCGTTATCACCGTGa > 1:693404/1‑37 (MQ=255) gtgtATTATTGTGAGCTGTTTCGCGTTATCACCGTGa > 1:4475818/1‑37 (MQ=255) gtgtATTATTGTGAGCTGTTTCGCGTTATCACCGTGATATGa > 1:2343300/1‑42 (MQ=255) tgtATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAg < 1:4508686/71‑1 (MQ=255) gtATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACAt > 1:1041129/1‑67 (MQ=255) tATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaaa < 1:1048289/58‑1 (MQ=255) aTTTTTGTGAGCTGTTTCGCGTTATCAACGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATc > 1:1189746/1‑71 (MQ=255) tttttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATaa < 1:3379463/55‑1 (MQ=255) ttttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGa > 1:1521308/1‑67 (MQ=255) ttttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGa > 1:4615048/1‑67 (MQ=255) ttttGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCt > 1:3263172/1‑70 (MQ=255) | CAGCAATAAACCCTTCAGTTTGCCAAACGGCGCTATTTTGTGTTGCAAAGA‑C‑CCCGTAAGCGTGTATTTTTGTGAGCTGTTTCGCGTTATCACCGTGATATGACACTCACTTTAAACATAAAATTAACATTAGATCT > W3110S.gb/4434281‑4434417 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |