| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,082 | T→C | 56.9% | I232I (ATT→ATC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,082 | 0 | T | C | 56.9% | 24.9 / 63.4 | 51 | I232I (ATT→ATC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (5/17); new base C (18/11); total (23/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.84e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGT > W3110S.gb/71018‑71148 | tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAg > 1:862084/1‑71 (MQ=255) gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg > 1:184423/1‑71 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGc > 1:706471/1‑71 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGa < 1:881256/71‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGa < 1:50492/71‑1 (MQ=255) ttttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgc > 1:1994297/1‑69 (MQ=255) tCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCa > 1:1978761/1‑58 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑tttt < 1:1107146/71‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGa < 1:1060487/71‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGACGAAGCTGC‑TTTTGa < 1:15846/71‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTg < 1:1166833/70‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAg > 1:1434085/1‑54 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTa > 1:468178/1‑49 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAg < 1:234164/71‑1 (MQ=255) agcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTgcgc < 1:1132136/71‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAg > 1:2178080/1‑58 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGAGAAg > 1:1622417/1‑58 (MQ=255) cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCa > 1:100983/1‑70 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑tt < 1:1704009/60‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑tt < 1:269037/60‑1 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAg < 1:1798790/65‑1 (MQ=255) tAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTAcc > 1:38106/1‑71 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCaccacc > 1:902312/1‑70 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCAccc > 1:533024/1‑71 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgc‑t < 1:1656406/55‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGa > 1:1878119/1‑71 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAg < 1:1965947/59‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg < 1:636536/41‑1 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGcc > 1:909879/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGc > 1:1670644/1‑70 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGcc > 1:1866459/1‑71 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑ttt < 1:1210404/50‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCg < 1:494681/62‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgct < 1:2445914/43‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAATCTGC‑tttt < 1:69328/49‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTg < 1:1020360/48‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCg < 1:1021688/59‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGc > 1:2241974/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGc > 1:1773592/1‑71 (MQ=255) tAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTg < 1:2534438/47‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGcc < 1:915396/70‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCa > 1:968964/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCa > 1:228817/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTTGAGCACTACCCGGa > 1:1584011/1‑60 (MQ=255) gCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCAcc < 1:325085/71‑1 (MQ=255) gCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCAcc < 1:1763541/71‑1 (MQ=255) cTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCg < 1:987292/71‑1 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCgtc < 1:2114991/70‑1 (MQ=255) cgcgAGGACCAACGTATCAGCCAGGCGAAgctgc‑t < 1:664068/35‑1 (MQ=25) gACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGa > 1:837221/1‑46 (MQ=255) gCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGt < 1:2119662/71‑1 (MQ=255) | TGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGT > W3110S.gb/71018‑71148 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |