| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,109 | T→C | 50.0% | T241T (ACT→ACC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,109 | 0 | T | C | 50.0% | ‑4.7 / 63.1 | 42 | T241T (ACT→ACC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (14/7); new base C (13/8); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.67e-01 | |||||||||||
TAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTA > W3110S.gb/71042‑71178 | tAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTAcc > 1:38106/1‑71 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCaccacc > 1:902312/1‑70 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCAccc > 1:533024/1‑71 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGa > 1:1878119/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGcc > 1:909879/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGc > 1:1670644/1‑70 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGcc > 1:1866459/1‑71 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCg < 1:494681/62‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCg < 1:1021688/59‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGc > 1:2241974/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGc > 1:1773592/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGcc < 1:915396/70‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCa > 1:968964/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCa > 1:228817/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTTGAGCACTACCCGGa > 1:1584011/1‑60 (MQ=255) gCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCAcc < 1:325085/71‑1 (MQ=255) gCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCAcc < 1:1763541/71‑1 (MQ=255) cTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCg < 1:987292/71‑1 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCgtc < 1:2114991/70‑1 (MQ=255) gACCAACGTATCAGCCAGGCGAAGCTGC‑TTTTGAGCACCACCCGGa > 1:837221/1‑46 (MQ=255) gCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGt < 1:2119662/71‑1 (MQ=255) aGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:1409125/1‑71 (MQ=255) gccAGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:936799/54‑1 (MQ=255) cAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGa > 1:1725042/1‑71 (MQ=255) aGGCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAt > 1:875907/1‑71 (MQ=255) ggCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:1140336/71‑1 (MQ=255) ggCGAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:2589449/71‑1 (MQ=255) gCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc > 1:1531346/1‑70 (MQ=255) gAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc > 1:1646258/1‑64 (MQ=255) gAAGCTGC‑TTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa > 1:1979416/1‑70 (MQ=255) aaGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCgtc < 1:1294031/42‑1 (MQ=255) aGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt > 1:2205255/1‑53 (MQ=255) aGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:1997620/1‑61 (MQ=255) ctgc‑tTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:1805366/45‑1 (MQ=255) tgc‑tTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAAt < 1:2378671/47‑1 (MQ=255) c‑tTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt > 1:2620382/1‑71 (MQ=255) c‑tTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt > 1:1976714/1‑71 (MQ=255) c‑tTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:826458/69‑1 (MQ=255) tttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCaa > 1:1301326/1‑42 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTgg > 1:1911617/1‑47 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt > 1:969842/1‑68 (MQ=255) taGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa > 1:867386/2‑58 (MQ=255) cTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTa > 1:1348924/1‑71 (MQ=255) tACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt < 1:2424782/37‑1 (MQ=255) | TAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGC‑TTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTA > W3110S.gb/71042‑71178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |