| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 97.6% | 137.6 / ‑3.8 | 42 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (20/21); total (20/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
CGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAT > W3110S.gb/547766‑547890 | cgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg‑ggg < 1:341211/71‑2 (MQ=255) gcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGaa > 1:2724306/1‑71 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAAc < 1:2379408/71‑1 (MQ=255) cATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAAc < 1:3532245/71‑1 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACtt < 1:2399996/71‑1 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGTGGAAGTGAACTTCa < 1:2074623/71‑1 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTc < 1:1789727/68‑1 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa > 1:173823/1‑65 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa > 1:3277348/1‑65 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCtt > 1:3110318/1‑71 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCt > 1:3334044/1‑70 (MQ=255) gAAGGTTGGACGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCtt < 1:1345562/70‑1 (MQ=255) aaGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTg < 1:1617309/70‑1 (MQ=255) ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCa > 1:2234629/1‑71 (MQ=255) gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCAtgt < 1:1718321/71‑1 (MQ=255) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGa < 1:1679413/71‑1 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg > 1:429073/1‑37 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:1735756/1‑71 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:2885573/1‑71 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:1175608/1‑71 (MQ=255) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc > 1:2669753/1‑71 (MQ=255) gAAGCGCAGGCGGTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg > 1:3324299/1‑70 (MQ=255) aaGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc > 1:1233226/1‑71 (MQ=255) aaGCGCAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc > 1:1256434/1‑71 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCa > 1:226216/1‑43 (MQ=38) cgcAGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg > 1:2695646/1‑71 (MQ=255) aGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa < 1:2125620/71‑1 (MQ=255) aGGCGCTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCa < 1:587829/71‑1 (MQ=255) gcgcTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt < 1:219691/71‑1 (MQ=255) cgcTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg < 1:2926476/71‑1 (MQ=255) cTGGCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCAtgt < 1:2962168/50‑1 (MQ=255) gCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg < 1:3175947/70‑1 (MQ=255) gCAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCg < 1:3526457/70‑1 (MQ=255) cAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt < 1:234060/71‑1 (MQ=255) cAATACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGt < 1:1240173/71‑1 (MQ=255) aaTACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg < 1:2259867/65‑1 (MQ=255) aTACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt > 1:575051/1‑71 (MQ=255) aTACTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:2848258/71‑1 (MQ=255) cTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCa > 1:1953765/1‑38 (MQ=38) cTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATg < 1:1418880/46‑1 (MQ=255) cTGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATg < 1:3587424/46‑1 (MQ=255) tGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg > 1:2910708/1‑53 (MQ=255) tGGAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCggg > 1:829417/1‑53 (MQ=255) gAGCAGGG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc > 1:1265204/1‑60 (MQ=255) | CGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GG‑GGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAT > W3110S.gb/547766‑547890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |