| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,394,860:1 | +G | 19.4% | coding (68/1131 nt) | fruB ← | fused fructose‑specific PTS enzyme IIA component and HPr component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,394,860 | 1 | . | G | 19.4% | 134.5 / 64.6 | 62 | coding (68/1131 nt) | fruB | fused fructose‑specific PTS enzyme IIA component and HPr component |
| Reads supporting (aligned to +/- strand): ref base . (17/33); new base G (8/4); total (25/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT > minE/1394792‑1394927 | gcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑g > 1:3430849/1‑70 (MQ=255)gcgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑Ga < 1:4009994/71‑1 (MQ=255) cgcCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑Gaa < 1:2321964/71‑1 (MQ=255) cAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCg > 1:379873/1‑71 (MQ=255) aGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCg < 1:3690740/71‑1 (MQ=255) gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGcc < 1:1702335/71‑1 (MQ=255) gCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGcc < 1:2116708/71‑1 (MQ=255) cATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCct > 1:906502/1‑71 (MQ=255) gCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGCctct > 1:2040592/1‑71 (MQ=255) ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCtcttc < 1:2150024/71‑1 (MQ=255) ccATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCtcttc < 1:2145236/71‑1 (MQ=255) cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCtcttct < 1:1023612/71‑1 (MQ=255) cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCtcttct < 1:2867687/71‑1 (MQ=255) cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCtcttct < 1:1921657/71‑1 (MQ=255) cATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCtcttct < 1:1627056/71‑1 (MQ=255) ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGCCTcttctt > 1:389881/1‑71 (MQ=255) ttGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAAt < 1:3924367/59‑1 (MQ=255) gACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGt > 1:1372039/1‑71 (MQ=255) aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGCCTCTTCTTTGTCTcc > 1:3871869/1‑71 (MQ=255) aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGCCTCTTCTTTGTCTcc > 1:2218867/1‑71 (MQ=255) aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGCCTCTTCTTTGTCTcc > 1:3460295/1‑71 (MQ=255) aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAAt > 1:135369/1‑52 (MQ=255) aGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCg > 1:3674063/1‑71 (MQ=255) ccTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGcc < 1:3402644/54‑1 (MQ=255) ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGCCTCTTCTTTGTCTCCGGcc > 1:872940/1‑71 (MQ=255) ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑gga < 1:2782013/47‑3 (MQ=255) ttCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑gga < 1:203149/47‑3 (MQ=255) ttCTGCTACATTACCGGCCTGCACCAGAGCCGCAGCGACCTGGC‑gga < 1:1131341/47‑3 (MQ=39) tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGc > 1:2388243/1‑51 (MQ=39) tCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGCGGAATCGc > 1:577833/1‑51 (MQ=39) cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCttt > 1:992700/1‑71 (MQ=255) cTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCtt < 1:1992016/70‑1 (MQ=255) cTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCttt < 1:1972627/68‑1 (MQ=255) cTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCttt < 1:158506/68‑1 (MQ=255) aCATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCttt > 1:3555977/1‑53 (MQ=255) cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGccc > 1:2350104/1‑71 (MQ=255) cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGc < 1:2788936/69‑1 (MQ=255) cATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGc < 1:2302581/69‑1 (MQ=255) aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCttt > 1:2301547/1‑64 (MQ=255) aTTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCg < 1:3578222/71‑1 (MQ=255) tACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCgga > 1:2410680/1‑71 (MQ=255) aCCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCggat > 1:3936789/1‑71 (MQ=255) ccGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatg < 1:1413954/71‑1 (MQ=255) cGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCggatgg < 1:3677689/71‑1 (MQ=255) ccTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGgatggatg < 1:3912591/71‑1 (MQ=255) ccTGCACCAGCGCCGCAGCGACATGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGgatggatg < 1:3307176/71‑1 (MQ=255) cTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCct > 1:2973884/1‑35 (MQ=255) aCCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTg < 1:2340820/71‑1 (MQ=255) ccAGCGCCGCAGCGACCTGGCGGAATCGCCTCTTCTTTGTCTCCGGc < 1:2038121/47‑1 (MQ=38) ccAGCGCCGCAGCGACCTGGCGGAATCGCCTCTTCTTTGTCTCCGGc < 1:529985/47‑1 (MQ=38) ccAGCGCCGCAGCGACCTGGCGGAATCGCCTCTTCTTTGTCTCCGGc < 1:1251377/47‑1 (MQ=38) gcgcCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACg > 1:3732378/1‑71 (MQ=255) gcgcCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACg > 1:433586/1‑71 (MQ=255) gccgcAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGa > 1:2111573/1‑71 (MQ=255) ccgcAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGAt < 1:3028805/71‑1 (MQ=255) cGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg < 1:3973457/71‑1 (MQ=255) cGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg < 1:3974690/71‑1 (MQ=255) cGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg < 1:297623/71‑1 (MQ=255) cGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg < 1:270939/71‑1 (MQ=255) cGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTgg < 1:1018714/71‑1 (MQ=255) aCCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa < 1:2746775/71‑1 (MQ=255) cTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa < 1:3312796/71‑1 (MQ=255) cTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACa < 1:424021/71‑1 (MQ=255) ggC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa > 1:371874/1‑67 (MQ=255) ggC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGaa > 1:3286524/1‑67 (MQ=255) gC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAt < 1:2075435/69‑1 (MQ=255) | GCGCCAGCATGCCATTGACGTAGCCTTCTGCTACATTACCGGCCTGCACCAGCGCCGCAGCGACCTGGC‑GAATCGCCTCTTCTTTGTCTCCGGCCTTTTCGCCCGGATGGATGTCCTGTACGGATAACTGGAACAT > minE/1394792‑1394927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |