| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 975,257:1 | +A | 17.0% | coding (358/375 nt) | ydeH ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 975,257 | 1 | . | A | 17.0% | 261.8 / 69.2 | 88 | coding (358/375 nt) | ydeH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (42/31); new base A (9/6); total (51/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
GAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGA > minE/975189‑975327 | gAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑a > 1:352161/1‑70 (MQ=255)gAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑At > 1:3532200/1‑71 (MQ=255) aaCCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑aat > 1:3562574/1‑69 (MQ=255) gacgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGtt < 1:335083/71‑1 (MQ=255) acgacCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa > 1:2570394/1‑71 (MQ=255) acCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGc < 1:167766/71‑1 (MQ=255) ccGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGcc > 1:2123206/1‑71 (MQ=255) ggCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCg > 1:2801930/1‑69 (MQ=255) gCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATc > 1:1163501/1‑57 (MQ=255) aaTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTa < 1:2510332/71‑1 (MQ=255) aTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTa > 1:4109807/1‑70 (MQ=255) aTCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTa > 1:3367122/1‑70 (MQ=255) tCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaaa < 1:1118899/71‑1 (MQ=255) cccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa > 1:1863951/1‑58 (MQ=255) cccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAg > 1:701782/1‑66 (MQ=255) cccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaaa > 1:2145325/1‑70 (MQ=255) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑a > 1:1937369/1‑49 (MQ=255) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGt > 1:804183/1‑54 (MQ=255) ccGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGt > 1:2943073/1‑54 (MQ=255) cGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:431374/71‑1 (MQ=255) cGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga < 1:823662/71‑1 (MQ=255) gTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga > 1:82762/1‑70 (MQ=255) gTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaaga > 1:75011/1‑70 (MQ=255) tCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAAt < 1:3264497/56‑1 (MQ=39) tCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaagaa < 1:407347/71‑1 (MQ=255) tCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaag < 1:3646041/68‑1 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaagaaag > 1:199698/1‑70 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:805298/71‑1 (MQ=255) aaaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:2016529/71‑1 (MQ=255) aaaCATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGt < 1:107772/42‑1 (MQ=255) cATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCaa > 1:3101824/1‑69 (MQ=255) aTCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaaaa < 1:3254364/60‑1 (MQ=255) tCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑a < 1:3532094/38‑1 (MQ=255) tCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCg > 1:1712360/1‑41 (MQ=255) tCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCg > 1:2312501/1‑41 (MQ=255) tCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt < 1:1479661/44‑1 (MQ=255) tCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa > 1:3247357/1‑46 (MQ=255) atatTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaag < 1:1180002/57‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaaga < 1:2402999/58‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAaaaga < 1:633079/58‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt < 1:6352/40‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt < 1:282496/40‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt < 1:1456259/40‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaa < 1:959395/53‑1 (MQ=255) tatTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGc < 1:1319666/61‑1 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCcctcct > 1:1873774/1‑70 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCg > 1:3693220/1‑35 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTaa > 1:135282/1‑40 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTa > 1:3661555/1‑39 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaagaa > 1:1771534/1‑56 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCaa > 1:667926/1‑61 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTg > 1:1773263/1‑70 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa < 1:179374/71‑1 (MQ=255) ttGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa < 1:1722203/71‑1 (MQ=255) gCTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATACCGCAGTAAAAGAAAGCAACCCCTCCTGa > 1:244399/1‑70 (MQ=255) gCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGtt > 1:3522991/1‑36 (MQ=255) gCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAAcc > 1:1918351/1‑61 (MQ=255) cTACGGATCGTCAGCAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa > 1:3116604/1‑69 (MQ=255) cTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAaaag < 1:3311559/51‑1 (MQ=255) tACGGATCGTCAGCAAATAAATTTTGTAATCGGTTaa > 1:1419218/1‑37 (MQ=37) cGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGc < 1:364552/70‑1 (MQ=255) cGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCATCCTGa < 1:1659/65‑1 (MQ=255) aTCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAATCaa > 1:3745843/1‑52 (MQ=255) tCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAgg > 1:2157865/1‑65 (MQ=255) tCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:2213885/71‑1 (MQ=255) cAGCAAATAAATTTTTT‑ATCGGTTAATGCCGCAGTaaaaca < 1:3923443/41‑3 (MQ=38) aGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATg < 1:2728124/70‑1 (MQ=255) cAAATAAATTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGa < 1:357755/55‑1 (MQ=255) cAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaaaa < 1:3330947/36‑1 (MQ=255) cAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTaaaa > 1:3928041/1‑36 (MQ=255) cAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCaa > 1:3998983/1‑44 (MQ=255) cAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgcgc > 1:3484274/1‑71 (MQ=255) cAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATgcgc > 1:839929/1‑71 (MQ=255) aataaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGt > 1:2669953/1‑71 (MQ=255) ataaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCcct < 1:1201145/46‑1 (MQ=255) ataaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGt > 1:2950555/1‑58 (MQ=255) ataaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGaaa < 1:2371072/63‑1 (MQ=255) ataaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGaaa < 1:1599181/63‑1 (MQ=255) ataaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGt < 1:1567351/70‑1 (MQ=255) ataaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTc > 1:2607781/1‑71 (MQ=255) taaatTTTGT‑ATCGGTTAATGCCGCAGTAaaagaa > 1:1827088/1‑35 (MQ=255) taaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTcc > 1:1140163/1‑71 (MQ=255) taaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTcc > 1:2082436/1‑71 (MQ=255) taaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTcc > 1:3894385/1‑71 (MQ=255) aaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCaa > 1:420448/1‑40 (MQ=38) aaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCaa > 1:294780/1‑40 (MQ=38) aaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTc > 1:3621409/1‑70 (MQ=255) aaatTTTGTAATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTc > 1:3387661/1‑70 (MQ=255) aaatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAAccc > 1:1181111/1‑42 (MQ=255) aatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAAccc > 1:2200019/1‑41 (MQ=255) aatTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCt < 1:2540613/70‑1 (MQ=255) ttGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGaaa < 1:3972727/56‑1 (MQ=255) ttGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAg > 1:2390880/1‑71 (MQ=255) t‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGa < 1:3098948/51‑1 (MQ=255) t‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGa < 1:892342/71‑1 (MQ=255) | GAACCCGACGACCCGGCAATCCCGTCAAAACATCCATATTGCTACGGATCGTCAGCAAATAAATTTTGT‑ATCGGTTAATGCCGCAGTAAAAGAAAGCAACCCCTCCTGAAAGGCGTCGAAATGCGCGTCCTGCCAGTGA > minE/975189‑975327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |