| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 821,576 | T→G | 21.9% | intergenic (‑113/+341) | ldrA ← / ← ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 821,576 | 0 | T | G | 21.9% | 57.5 / 17.5 | 32 | intergenic (‑113/+341) | ldrA/ldrC | toxic polypeptide, small/toxic polypeptide, small |
| Reads supporting (aligned to +/- strand): ref base T (11/14); new base G (3/4); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAA > minE/821506‑821643 | aaTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt > 1:1624421/1‑71 (MQ=34) aTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt < 1:3822530/71‑1 (MQ=34) aTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt > 1:3195340/1‑70 (MQ=35) aTGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt < 1:3179352/70‑1 (MQ=35) acaacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAcc > 1:294815/1‑71 (MQ=34) caTTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCtctc < 1:1452934/71‑1 (MQ=11) tGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACg < 1:1620400/70‑1 (MQ=11) gCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt < 1:4028386/58‑1 (MQ=35) cGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:3578907/70‑1 (MQ=35) cGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg < 1:1608141/70‑1 (MQ=35) cTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACct > 1:2128424/1‑58 (MQ=35) cTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACct > 1:3029150/1‑58 (MQ=35) cTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCggggg < 1:1538781/68‑1 (MQ=35) ttGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGtt < 1:1778157/69‑1 (MQ=11) gAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGtttt < 1:3080060/69‑1 (MQ=35) gAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtc < 1:3526143/70‑1 (MQ=35) gtg‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt < 1:556394/43‑1 (MQ=38) tg‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt < 1:3424958/41‑1 (MQ=38) tg‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTAAGGTTTTACCTCTCaa < 1:893060/51‑1 (MQ=255) tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACg > 1:103941/1‑51 (MQ=35) cTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCtctc < 1:4014743/46‑1 (MQ=38) gTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCa < 1:1063978/71‑1 (MQ=34) tCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:4119763/1‑35 (MQ=40) tCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc > 1:105460/1‑35 (MQ=40) cAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAAcc < 1:929802/70‑1 (MQ=25) aGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCg < 1:239473/45‑1 (MQ=38) aTTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCaa > 1:591947/1‑68 (MQ=35) aGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtc > 1:3145238/1‑49 (MQ=38) gCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGccac < 1:3844609/70‑1 (MQ=35) gCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGccac < 1:1566700/70‑1 (MQ=35) cccccGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGcaac > 1:906797/1‑69 (MQ=25) ccGTCCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtc < 1:3622396/44‑1 (MQ=255) cGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCaa > 1:3990135/1‑55 (MQ=35) gTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGtttt > 1:551319/1‑41 (MQ=38) ttCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGAt < 1:3810372/70‑1 (MQ=35) cTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCaa > 1:3268292/1‑55 (MQ=9) cTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCaa > 1:1626369/1‑55 (MQ=9) aGGTTGTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCg > 1:2422109/1‑70 (MQ=18) gTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCaa < 1:188836/71‑1 (MQ=38) | AATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAA > minE/821506‑821643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |