| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 189,641 | A→G | 11.2% | I380V (ATT→GTT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 189,641 | 0 | A | G | 11.2% | 218.0 / 17.5 | 80 | I380V (ATT→GTT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (35/36); new base G (5/4); total (40/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
AGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGT > minE/189574‑189709 | aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:252468/71‑1 (MQ=255)aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:3511725/71‑1 (MQ=255)aGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:2083622/71‑1 (MQ=255) gTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTaa < 1:3872704/71‑1 (MQ=255) tGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTaaa < 1:3674234/71‑1 (MQ=255) tGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa > 1:3374996/1‑69 (MQ=255) cTTCGCAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg > 1:4006338/1‑69 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc < 1:952189/67‑1 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:2787942/1‑70 (MQ=255) cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g < 1:3536201/70‑1 (MQ=255) gAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑Gt < 1:2764191/70‑1 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTAt < 1:3308721/71‑1 (MQ=255) aaaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTAt < 1:3790587/71‑1 (MQ=255) aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc < 1:273207/64‑1 (MQ=255) aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGa > 1:2706938/1‑71 (MQ=255) cGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACAt < 1:2146877/55‑1 (MQ=255) cGCAATCGACCCACAAAATGCTGGCGGCGTTATAGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGAc > 1:2076044/1‑71 (MQ=255) aaTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAGGGCGA‑GTATGACGa < 1:2699099/70‑1 (MQ=255) aaTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:3639271/71‑1 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:1676378/52‑1 (MQ=255) tCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g < 1:272144/60‑1 (MQ=255) cGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑g > 1:3371089/1‑59 (MQ=255) cGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAagag > 1:3630981/1‑71 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc < 1:1569402/55‑1 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAagag < 1:2897260/70‑1 (MQ=255) gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAaga > 1:736254/1‑69 (MQ=255) cccACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg > 1:1754145/1‑52 (MQ=255) cccACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGcc > 1:766665/1‑71 (MQ=255) tcacaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTaa < 1:1792850/47‑1 (MQ=16) cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaaa < 1:3768577/48‑1 (MQ=255) cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:3719236/46‑1 (MQ=255) cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTa < 1:3724012/46‑1 (MQ=255) cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCtt > 1:2948334/1‑71 (MQ=255) cacaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCtt < 1:1941925/71‑1 (MQ=255) acaAACTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAg > 1:2122352/1‑48 (MQ=19) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCcaca > 1:252244/1‑41 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg > 1:363569/1‑48 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg > 1:769940/1‑48 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAgg > 1:3121588/1‑48 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGc < 1:2507303/49‑1 (MQ=255) caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTa > 1:3151360/1‑54 (MQ=255) aaacTGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAg > 1:3548272/1‑46 (MQ=19) aaaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAaga > 1:3413046/1‑62 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTaa > 1:1479120/1‑43 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAAc < 1:547807/71‑1 (MQ=255) aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAAc > 1:3269386/1‑71 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑TTATGACGAAGAGGCCTTTAACg < 1:407097/71‑1 (MQ=255) aaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACg < 1:120559/71‑1 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:2777367/56‑1 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:2417675/56‑1 (MQ=255) tGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGaa < 1:3868561/56‑1 (MQ=255) gCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAg > 1:3504986/1‑71 (MQ=12) gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCta < 1:2406988/71‑2 (MQ=9) gcggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGcc < 1:1372940/69‑1 (MQ=255) ggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCttt < 1:651137/70‑1 (MQ=255) gcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat < 1:3953913/71‑1 (MQ=255) gcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat < 1:883548/71‑1 (MQ=255) gcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat < 1:3881690/71‑1 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAagag > 1:945348/1‑46 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCt > 1:1069056/1‑50 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAAc > 1:3932462/1‑55 (MQ=255) aTCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg < 1:2064230/70‑1 (MQ=255) cAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTa < 1:2103034/61‑1 (MQ=255) aGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATac > 1:181881/1‑70 (MQ=255) ttCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGaa > 1:3954594/1‑49 (MQ=255) ttCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTat < 1:1969773/57‑1 (MQ=255) ttCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATa > 1:2795776/1‑65 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCt > 1:3852598/1‑51 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATagcacca < 1:2899388/69‑1 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc > 1:2828082/1‑71 (MQ=255) cGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccac > 1:2562854/1‑67 (MQ=255) gCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGc > 1:3038132/1‑48 (MQ=255) gCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGc > 1:4004722/1‑48 (MQ=255) atGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc > 1:1223342/2‑71 (MQ=9) tGATCCACGTTAAAGGTGACGTA‑AACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTct > 1:3372426/1‑70 (MQ=9) tGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTaa > 1:1317893/1‑40 (MQ=255) tGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccac > 1:105536/1‑67 (MQ=255) tGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccac > 1:1971569/1‑67 (MQ=255) gATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTaa > 1:95492/1‑39 (MQ=255) tCCACATTAAAGGCGA‑GTATGACGAAGAGGCCttt > 1:2660156/1‑35 (MQ=255) ccACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTatga > 1:1816185/1‑50 (MQ=255) acaTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGt < 1:2056686/71‑1 (MQ=255) caTTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGt > 1:197777/1‑70 (MQ=255) | AGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGA‑GTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGT > minE/189574‑189709 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |