| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,279 | T→A | 7.1% | V1246E (GTA→GAA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,279 | 0 | T | A | 7.1% | 319.3 / 13.8 | 113 | V1246E (GTA→GAA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (40/65); new base A (6/2); total (46/67) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.07e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 115 total aligned reads displayed.
GTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTT > minE/835212‑835349 | gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa < 1:4080190/70‑1 (MQ=255)gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa < 1:1597182/70‑1 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa < 1:804020/71‑1 (MQ=255) tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taa < 1:3671209/69‑1 (MQ=255) gTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATg < 1:323748/71‑1 (MQ=255) tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAAt < 1:2971032/71‑1 (MQ=255) aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:1499077/1‑71 (MQ=255) aTGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGa < 1:1567267/63‑1 (MQ=255) ttaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt > 1:2154045/3‑71 (MQ=255) tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt < 1:319193/71‑1 (MQ=255) aagaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTca > 1:858897/1‑71 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:2883549/70‑1 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:1757746/70‑1 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:4019999/70‑1 (MQ=255) agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcac < 1:1250350/71‑1 (MQ=255) agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:2396118/71‑1 (MQ=255) agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:3708720/71‑1 (MQ=255) agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:3108867/71‑1 (MQ=255) gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:2240454/70‑1 (MQ=255) aaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc < 1:3636421/63‑1 (MQ=255) aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAg < 1:126830/71‑1 (MQ=255) aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAg < 1:1157365/71‑1 (MQ=255) cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGt < 1:1872410/71‑1 (MQ=255) aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:426998/66‑1 (MQ=255) aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:1300697/66‑1 (MQ=255) aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc < 1:3359358/71‑1 (MQ=255) ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc < 1:2556883/70‑1 (MQ=255) tGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg > 1:1462467/1‑71 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑Taaaa > 1:1341279/1‑48 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:1879892/70‑1 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:489407/70‑1 (MQ=255) cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcaca > 1:932227/1‑62 (MQ=255) ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg > 1:104270/1‑71 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAg < 1:326096/58‑1 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc < 1:1468284/70‑1 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc < 1:3769537/70‑1 (MQ=255) gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg < 1:1145921/71‑1 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:3995761/1‑58 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:864810/1‑58 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:2464507/1‑58 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:2099645/60‑1 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:2427586/60‑1 (MQ=255) aTGGCGAAGGCAATGA‑CAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:1343662/1‑57 (MQ=255) tGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg < 1:3949191/66‑1 (MQ=255) ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt > 1:1741163/1‑71 (MQ=255) ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt > 1:1561820/1‑71 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGaa < 1:2820440/39‑1 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc < 1:2442591/71‑1 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTca > 1:2752775/1‑48 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct < 1:1533646/71‑1 (MQ=255) aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc < 1:2869373/68‑1 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:538969/71‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:2928468/44‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg < 1:315717/55‑1 (MQ=255) cAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAAt < 1:972866/35‑1 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt < 1:3007371/62‑1 (MQ=255) aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:3008300/71‑1 (MQ=255) tGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:3702460/40‑1 (MQ=255) gACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTca > 1:2146996/1‑38 (MQ=255) gACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTg > 1:2091815/1‑70 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:271334/71‑1 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:3325566/1‑71 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:3291216/1‑71 (MQ=255) aCCAGGTAAAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:3377333/1‑71 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTc > 1:146600/2‑42 (MQ=12) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTaa > 1:1715360/2‑56 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTaa > 1:1778164/2‑56 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTaa > 1:2259667/2‑56 (MQ=255) cAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCCCAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATc < 1:412281/71‑1 (MQ=255) cAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg < 1:1992091/60‑1 (MQ=255) cAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATc < 1:3986898/71‑1 (MQ=255) aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATgg < 1:4096275/47‑1 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc < 1:3340659/70‑1 (MQ=255) ggTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg > 1:3315964/1‑70 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGc < 1:1710886/41‑1 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt < 1:926956/43‑1 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt < 1:1538958/43‑1 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg < 1:1424238/50‑1 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTg > 1:1295622/1‑64 (MQ=255) tACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:2213868/1‑54 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCa > 1:2820936/1‑40 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:1701981/1‑41 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:3472985/1‑41 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg > 1:37773/1‑45 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:3697308/1‑65 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:1720348/1‑71 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:650800/1‑71 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCCGc > 1:358216/1‑39 (MQ=255) cAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt > 1:3197154/1‑50 (MQ=255) cAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGc < 1:1878940/71‑1 (MQ=255) aGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:2451042/55‑1 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt < 1:3417621/37‑1 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:2335326/1‑37 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa > 1:56236/1‑55 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:1536728/1‑60 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:2403855/1‑60 (MQ=255) agagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcaca < 1:2470985/71‑1 (MQ=255) gagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:53865/1‑50 (MQ=255) cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTg < 1:456784/71‑1 (MQ=255) tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:1742712/1‑39 (MQ=255) | GTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTT > minE/835212‑835349 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |