| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 835,291 | T→C | 9.2% | I3I (ATT→ATC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 835,291 | 0 | T | C | 9.2% | 304.3 / 18.9 | 109 | I3I (ATT→ATC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (42/57); new base C (8/2); total (50/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
AGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAC > minE/835222‑835356 | aGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc > 1:1499077/1‑71 (MQ=255) ttaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt > 1:2154045/3‑71 (MQ=255) tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGtt < 1:319193/71‑1 (MQ=255) aagaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTca > 1:858897/1‑71 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:1757746/70‑1 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:4019999/70‑1 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:2883549/70‑1 (MQ=255) aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTc < 1:2921813/70‑1 (MQ=255) agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcac < 1:1250350/71‑1 (MQ=255) agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:2396118/71‑1 (MQ=255) agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:3708720/71‑1 (MQ=255) gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:2240454/70‑1 (MQ=255) aaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTc < 1:3636421/63‑1 (MQ=255) aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAg < 1:126830/71‑1 (MQ=255) aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAg < 1:1157365/71‑1 (MQ=255) cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGt < 1:1872410/71‑1 (MQ=255) aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:1300697/66‑1 (MQ=255) aTTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:426998/66‑1 (MQ=255) ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc < 1:2556883/70‑1 (MQ=255) tGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg > 1:1462467/1‑71 (MQ=255) gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:1879892/70‑1 (MQ=255) ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg > 1:104270/1‑71 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAg < 1:326096/58‑1 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc < 1:1468284/70‑1 (MQ=255) aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc < 1:3769537/70‑1 (MQ=255) gATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg < 1:1145921/71‑1 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:3995761/1‑58 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:864810/1‑58 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:2464507/1‑58 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:2099645/60‑1 (MQ=255) aTGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCgg < 1:2427586/60‑1 (MQ=255) aTGGCGAAGGCAATGA‑CAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTc > 1:1343662/1‑57 (MQ=255) tGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTg < 1:3949191/66‑1 (MQ=255) ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt > 1:1561820/1‑71 (MQ=255) ggCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt > 1:1741163/1‑71 (MQ=255) gCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc < 1:2442591/71‑1 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTca > 1:2752775/1‑48 (MQ=255) cGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct < 1:1533646/71‑1 (MQ=255) aaGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc < 1:2869373/68‑1 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:3187346/71‑1 (MQ=255) ggCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:538969/71‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:2928468/44‑1 (MQ=255) gCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATg < 1:315717/55‑1 (MQ=255) aaTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt < 1:3007371/62‑1 (MQ=255) aTGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGt < 1:3008300/71‑1 (MQ=255) tGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTcac < 1:3702460/40‑1 (MQ=255) gACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTca > 1:2146996/1‑38 (MQ=255) gACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTg > 1:2091815/1‑70 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:3291216/1‑71 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:3325566/1‑71 (MQ=255) aCCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa < 1:271334/71‑1 (MQ=255) aCCAGGTAAAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:3377333/1‑71 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTc > 1:146600/2‑42 (MQ=12) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTaa > 1:1715360/2‑56 (MQ=255) tcAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTaa > 1:1778164/2‑56 (MQ=255) cAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCCCAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATc < 1:412281/71‑1 (MQ=255) cAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg < 1:1992091/60‑1 (MQ=255) cAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATc < 1:3986898/71‑1 (MQ=255) cAGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTcaca > 1:3893664/1‑37 (MQ=9) aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATgg < 1:4096275/47‑1 (MQ=255) aGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg < 1:3659142/71‑1 (MQ=255) aGGTACAGGAG‑GCGAAAAAATGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATc < 1:3340659/70‑1 (MQ=255) ggTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCg > 1:3315964/1‑70 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt < 1:926956/43‑1 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt < 1:1538958/43‑1 (MQ=255) gTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTg > 1:1295622/1‑64 (MQ=255) tACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtct > 1:2213868/1‑54 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCa > 1:2820936/1‑40 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:1701981/1‑41 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:3472985/1‑41 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCAt > 1:3697308/1‑65 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:650800/1‑71 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTg > 1:1720348/1‑71 (MQ=255) aCAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCCGc > 1:358216/1‑39 (MQ=255) cAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:3334287/1‑49 (MQ=255) cAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAt > 1:3197154/1‑50 (MQ=255) cAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGc < 1:1878940/71‑1 (MQ=255) cAGGAGAGCG‑TAAAATGAAAATTCGATCACACGTCGTCATGGTGCTGAAtc > 1:2101013/1‑51 (MQ=255) aGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtctc < 1:2356822/52‑1 (MQ=255) aGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:2451042/55‑1 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt < 1:3417621/37‑1 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCAt > 1:2335326/1‑37 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa > 1:56236/1‑55 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:1536728/1‑60 (MQ=255) gagaGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCa > 1:2403855/1‑60 (MQ=255) agagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCcaca < 1:2470985/71‑1 (MQ=255) gagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:742718/1‑50 (MQ=255) gagCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa > 1:53865/1‑50 (MQ=255) cG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTg < 1:456784/71‑1 (MQ=255) tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGaa > 1:1742712/1‑39 (MQ=255) tAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGtt > 1:1315777/1‑71 (MQ=255) aaaaaTGAAAATCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGtt > 1:1565500/2‑71 (MQ=255) aaaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCa < 1:3013980/71‑1 (MQ=255) aaaTGAAAATTCGTTCACAAATCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTc < 1:2036912/70‑1 (MQ=255) aaTGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCTGCTGCCACACCTGTTCa < 1:1130553/70‑1 (MQ=255) tGAAAATCCGTACACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGt > 1:760565/1‑70 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGTCTGCCACACCTGTTCAGTTAc > 1:1714717/1‑71 (MQ=255) aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc > 1:525186/1‑71 (MQ=255) aTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATa < 1:2149539/37‑1 (MQ=255) ttCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt < 1:4516/35‑1 (MQ=255) | AGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCG‑TAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAC > minE/835222‑835356 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |