| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 195,592 | G→A | 18.0% | T79T (ACC→ACT) | yaeF ← | predicted lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 195,592 | 0 | G | A | 18.0% | 170.7 / 28.7 | 72 | T79T (ACC→ACT) | yaeF | predicted lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (36/23); new base A (10/3); total (46/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.58e-01 | |||||||||||
CGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTA > minE/195522‑195659 | cGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAg > 1:2819186/1‑71 (MQ=255) attatCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGtt > 1:1684319/1‑70 (MQ=255) attatCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTa > 1:3915835/1‑71 (MQ=255) attatCACCCAGAAAGATCGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTa > 1:3205393/1‑71 (MQ=255) tCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTAccc > 1:403885/1‑70 (MQ=255) aCCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTAcccc > 1:2360157/1‑69 (MQ=255) aCCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTAcccc > 1:3240370/1‑69 (MQ=255) aCCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTAcccc > 1:1954847/1‑69 (MQ=255) ccAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAg > 1:1957409/1‑70 (MQ=255) ccAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAgg > 1:3546783/1‑71 (MQ=255) ccAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAgg > 1:348189/1‑71 (MQ=255) gAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAg < 1:1953243/56‑1 (MQ=255) aaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAg > 1:4015320/1‑54 (MQ=255) aaGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTg < 1:2465691/70‑1 (MQ=255) aGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTgag < 1:1771730/71‑1 (MQ=255) aTTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAGGCTTgagga < 1:3802648/71‑1 (MQ=255) ttGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAg < 1:35619/50‑1 (MQ=255) ttGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTgag > 1:4128260/1‑68 (MQ=255) ttGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTgag > 1:483203/1‑68 (MQ=255) ttGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAGGCTTgagga < 1:2156269/70‑1 (MQ=255) tGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTa > 1:1951418/1‑53 (MQ=255) tGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTgaggag > 1:3589599/1‑70 (MQ=255) tGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTgaggag > 1:2709755/1‑70 (MQ=255) aaCGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTa > 1:1243353/1‑50 (MQ=255) gTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGc > 1:3421789/1‑70 (MQ=255) gTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAGGCTTGAGGAGAACAGc > 1:741014/1‑70 (MQ=255) aCTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCaaa < 1:2065138/70‑1 (MQ=255) cTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCaaa < 1:650737/69‑1 (MQ=255) tCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTAcccc < 1:3644695/46‑1 (MQ=255) acaGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGcc < 1:3431942/71‑1 (MQ=255) agagGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACa > 1:4042712/1‑59 (MQ=255) agagGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACa > 1:3814706/1‑59 (MQ=255) agGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTgagga > 1:421198/1‑52 (MQ=255) agGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAGGCtt > 1:2750809/1‑47 (MQ=255) agGAAGTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAGGCtt > 1:4073991/1‑47 (MQ=255) ggAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCt > 1:4005189/1‑45 (MQ=255) gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGttt > 1:4118584/1‑71 (MQ=255) gAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGtt > 1:3376521/1‑70 (MQ=255) aGTGCTGAAGACGCTGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga < 1:2248324/71‑1 (MQ=255) aGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga < 1:1613945/71‑1 (MQ=255) aGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga < 1:569385/71‑1 (MQ=255) gTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTg < 1:353237/43‑1 (MQ=255) gTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTg < 1:2625311/43‑1 (MQ=255) gTGCTGAAGACGCGGATTCCAAATGAAGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTgag < 1:1064089/71‑1 (MQ=255) tGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAgg > 1:480434/1‑38 (MQ=255) tGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGcc < 1:776555/61‑1 (MQ=255) gCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑aa > 1:2513833/1‑35 (MQ=255) gCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGaa > 1:1910507/1‑48 (MQ=255) gCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGttt > 1:2943710/1‑66 (MQ=255) gCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTgag > 1:1252817/1‑69 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTg > 1:245518/1‑40 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTg < 1:2657398/40‑1 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga > 1:3408482/1‑67 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga > 1:4128998/1‑67 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATc < 1:125595/71‑1 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATc < 1:2012137/71‑1 (MQ=255) cTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATc < 1:1431243/71‑1 (MQ=255) aaGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGAt > 1:3462184/1‑67 (MQ=255) aaGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGAt < 1:1780794/67‑1 (MQ=255) aaGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCa < 1:2789065/69‑1 (MQ=255) gACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga > 1:3058229/1‑62 (MQ=255) gACGCGGATTCCAAATGAAGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTg > 1:944634/1‑71 (MQ=255) tCCAAATGAGGTTACCCC‑ATGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGAt < 1:802704/56‑1 (MQ=255) cAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATc > 1:3919163/1‑55 (MQ=255) cAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATc > 1:930825/1‑55 (MQ=255) cAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCa > 1:2984758/1‑56 (MQ=255) cAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTc < 1:1813397/70‑1 (MQ=255) cAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCt < 1:3689429/71‑1 (MQ=255) cAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCt > 1:3430828/1‑71 (MQ=255) cAAATGAAGTTACCCC‑AAGGCTTGAGGAGAACAGCAAAt > 1:1585332/1‑39 (MQ=255) cAAATGAAGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCg > 1:138421/1‑44 (MQ=255) aaTGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTgag < 1:1762329/50‑1 (MQ=255) tGAGGTTACCCCTAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCtttt < 1:1843147/71‑1 (MQ=255) tGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGttt > 1:3628787/1‑45 (MQ=255) tGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTa > 1:3271512/1‑71 (MQ=255) aGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGttt > 1:1024316/1‑43 (MQ=255) ggTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTga > 1:1169448/1‑44 (MQ=255) | CGTTATTATCACCCAGAAAGATTGCAACGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCC‑AAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTA > minE/195522‑195659 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |