| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,002,423 | A→G | 11.1% | intergenic (+70/+53) | yghU → / ← hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,002,423 | 0 | A | G | 11.1% | 142.9 / 9.9 | 54 | intergenic (+70/+53) | yghU/hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base A (31/17); new base G (2/4); total (33/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01 | |||||||||||
AGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATG > minE/2002353‑2002491 | aGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGca > 1:548887/1‑71 (MQ=255)aGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGca > 1:1203569/1‑71 (MQ=255) gggTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcac < 1:3378073/71‑1 (MQ=255) ggTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca < 1:2419128/71‑1 (MQ=255) gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACa‑a > 1:4128386/1‑71 (MQ=255) ttGGTGTTCGTCGAAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑At < 1:2701731/71‑1 (MQ=255) ggTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca > 1:503713/1‑67 (MQ=255) gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGcc > 1:1680752/1‑71 (MQ=255) tcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCt < 1:361452/65‑1 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGa < 1:1654223/70‑1 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc < 1:2573098/71‑1 (MQ=255) caAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑Tg < 1:780812/71‑1 (MQ=255) caAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑Tg > 1:2704967/1‑71 (MQ=255) gCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCcg > 1:2290020/1‑71 (MQ=255) cATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCcgcg < 1:2206894/71‑1 (MQ=255) tCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTc > 1:346817/1‑71 (MQ=255) cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcac < 1:2568194/43‑1 (MQ=255) cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACa‑a > 1:1316842/1‑45 (MQ=255) cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCt > 1:330139/1‑70 (MQ=255) cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCt > 1:631762/1‑70 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:3448722/71‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt > 1:3210887/1‑70 (MQ=255) ccGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATc < 1:3412310/71‑1 (MQ=1) aTAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAgac > 1:524631/1‑69 (MQ=1) aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:2463435/65‑1 (MQ=255) tAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGGcc > 1:1050367/1‑71 (MQ=1) aaGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTa > 1:1020433/1‑71 (MQ=255) aGGCGTTCACGCCGCATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCt < 1:358397/70‑1 (MQ=11) aGGCGTTCACGCCGCATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCt < 1:2080616/70‑1 (MQ=11) aGGCGTTCACGCCGCATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCTa < 1:3671303/71‑1 (MQ=11) aGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGt > 1:214711/1‑57 (MQ=255) gCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGa < 1:728772/71‑1 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:3795835/1‑71 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaaa > 1:3046893/1‑68 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:476258/1‑70 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:3937802/1‑70 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:2190678/1‑70 (MQ=255) cACGCCGCATCCGGCAATAGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:3380385/1‑70 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCtt < 1:3361950/47‑1 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:2505782/71‑1 (MQ=255) cATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcatc < 1:1398609/71‑1 (MQ=255) tCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:1065760/1‑61 (MQ=255) tCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:826696/1‑61 (MQ=255) tCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAc < 1:684659/71‑1 (MQ=255) ggCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAat < 1:2658903/62‑1 (MQ=255) gCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTa < 1:3133943/59‑1 (MQ=255) cAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCt > 1:805523/1‑71 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg < 1:780395/71‑1 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:91636/1‑71 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:1380857/1‑71 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:3803643/1‑71 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg > 1:1106905/1‑70 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg > 1:835131/1‑70 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg > 1:3504646/1‑69 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGt > 1:266023/1‑70 (MQ=255) gTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAAtcatca > 1:3856057/1‑60 (MQ=255) gTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa > 1:2252628/1‑71 (MQ=255) gTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTa > 1:1189344/1‑70 (MQ=255) gTGCACA‑ATGCATGATGCGACGC‑TGACGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa < 1:3185941/71‑1 (MQ=255) gCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg > 1:674185/1‑71 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAc > 1:877467/1‑58 (MQ=255) | AGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATG > minE/2002353‑2002491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |