| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,493,839 | A→C | 16.4% | intergenic (+221/‑154) | gyrB → / → ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,493,839 | 0 | A | C | 16.4% | 151.4 / 36.1 | 82 | intergenic (+221/‑154) | gyrB/ibpA | DNA gyrase, subunit B/heat shock chaperone |
| Reads supporting (aligned to +/- strand): ref base A (41/27); new base C (6/7); total (48/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCCTTTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAG > minE/2493771‑2493908 | cGCCCTTTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑aa < 1:1831793/70‑1 (MQ=255) tttATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaaaa < 1:3770772/70‑1 (MQ=255) tttATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAg > 1:426996/1‑71 (MQ=255) tttATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAg > 1:4083432/1‑71 (MQ=255) cAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCTCTGAt < 1:2842518/70‑1 (MQ=255) cAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGAt > 1:3624888/1‑70 (MQ=255) cGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaaaa > 1:1404930/1‑55 (MQ=255) cGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGt > 1:2178522/1‑57 (MQ=255) cGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGt > 1:3492534/1‑57 (MQ=255) cGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCt > 1:3551498/1‑71 (MQ=255) cGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCt > 1:3801443/1‑71 (MQ=255) cAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGc < 1:2114641/68‑1 (MQ=255) aaTTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGc < 1:2360834/67‑1 (MQ=255) aTTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGaa < 1:1235895/71‑1 (MQ=255) ttATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaa < 1:1786134/48‑1 (MQ=255) ttATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCt < 1:738898/57‑1 (MQ=255) tATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGaa > 1:1267758/1‑69 (MQ=255) tATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGaa > 1:2683639/1‑69 (MQ=255) atcatcCCGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaa < 1:1821587/46‑1 (MQ=255) atcatcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAg > 1:643664/1‑49 (MQ=255) atcatcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAg > 1:2207226/1‑49 (MQ=255) tcatcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg < 1:810045/70‑1 (MQ=255) tcatcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg < 1:4110706/70‑1 (MQ=255) tcatcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGt < 1:483489/71‑1 (MQ=255) catcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATaa > 1:2696926/1‑58 (MQ=255) catcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGTCTTGAAAAGt > 1:381384/1‑70 (MQ=255) tcCCGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaaaa < 1:3008009/44‑1 (MQ=255) tcCCGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaaaa < 1:139883/44‑1 (MQ=255) tcCCGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaaa < 1:3666528/43‑1 (MQ=255) tcCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATaa > 1:273351/1‑56 (MQ=255) cccGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAt > 1:356707/1‑71 (MQ=255) ccGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAtt > 1:3212490/1‑71 (MQ=255) ccGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCAtt > 1:2582823/1‑71 (MQ=255) cGCAAATCTACTATGCAATAGACCATAAACTG‑aaaaaaa < 1:2015466/39‑1 (MQ=255) cAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg < 1:2860538/62‑1 (MQ=255) aaTCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTc > 1:3647727/1‑68 (MQ=255) agtctaTGCAATAGACCATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGAc > 1:2027875/4‑70 (MQ=255) gtctaTGCAATAGACCATAAACTG‑CAAAAAAAAGTCCGCt < 1:1253055/38‑1 (MQ=39) gtctaTGCAATAGACCATAAACTG‑CAAAAAAAAGTCCGCt < 1:2709656/38‑1 (MQ=39) gtctaTGCAATAGACCATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGt > 1:454810/3‑57 (MQ=255) tctaTGCAATAGACCATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGaaa < 1:2446124/52‑1 (MQ=255) ctaTGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAt > 1:796032/1‑71 (MQ=255) ctaTGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAt < 1:1107748/71‑1 (MQ=255) ctaTGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTCAAAAGTTCATTTCCAGACCCAt > 1:181507/1‑71 (MQ=255) taTGCAATAGACCATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTcc > 1:565889/1‑62 (MQ=255) tGCAATAGACCATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtt < 1:2063051/69‑1 (MQ=255) tGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATaa > 1:3561493/1‑40 (MQ=255) tGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAttt > 1:661346/1‑70 (MQ=255) tGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtt < 1:777433/69‑1 (MQ=255) tGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAtt < 1:2236560/69‑1 (MQ=255) tGCAATAGACCATAAACTG‑‑CAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAttt > 1:287905/1‑69 (MQ=255) gCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTg < 1:432638/35‑1 (MQ=255) cAATAGACCATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTa < 1:1721096/71‑1 (MQ=255) cAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:964335/1‑48 (MQ=255) cAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAg < 1:395891/49‑1 (MQ=255) cAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAttttt < 1:3392764/70‑1 (MQ=255) cAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTa > 1:4040170/1‑71 (MQ=255) cAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTa < 1:109150/71‑1 (MQ=255) aTAGACCATAAACTGAAAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTAc < 1:3446644/71‑1 (MQ=255) aTAGACCATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:2314872/1‑46 (MQ=255) aTAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAt < 1:709280/64‑1 (MQ=255) tAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACa > 1:999857/1‑70 (MQ=255) aGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:3839751/1‑44 (MQ=255) aGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:1051532/1‑44 (MQ=255) aGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATc > 1:1016831/1‑71 (MQ=255) aGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATc > 1:4013650/1‑71 (MQ=255) gACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGaaa > 1:1409729/1‑42 (MQ=255) gACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCg > 1:64342/1‑71 (MQ=255) gACCATAAACTG‑AAAAAAAAAGTACGCTGATAAGGCTTGaaaa > 1:493980/1‑43 (MQ=255) aCCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTcc < 1:1052421/52‑1 (MQ=255) ccATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGaaaa > 1:1796290/1‑41 (MQ=255) cATAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAg > 1:589389/1‑71 (MQ=255) cATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAg < 1:3766469/71‑1 (MQ=255) tAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGaa < 1:1407232/36‑1 (MQ=255) tAAACTG‑CAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc < 1:1406363/71‑1 (MQ=255) tAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTc < 1:4008613/42‑1 (MQ=255) aaaCTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCAttt > 1:3167680/1‑57 (MQ=255) aaaCTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc > 1:3242298/1‑70 (MQ=255) aaaCTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGcc > 1:864856/1‑70 (MQ=255) aaaCTG‑AAAAAAAAAGTCCGCTGATAAGGCTT‑AAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGa > 1:3399266/1‑71 (MQ=255) aaCTT‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCg < 1:134451/70‑1 (MQ=255) aaCTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGc > 1:3693697/1‑68 (MQ=255) aaCTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGa > 1:1380315/1‑71 (MQ=255) aaCTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGa > 1:915461/1‑71 (MQ=255) aCTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAt < 1:2007521/71‑1 (MQ=255) cTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAt > 1:2271814/1‑70 (MQ=255) cTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATg < 1:2812071/71‑1 (MQ=255) cTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATg < 1:1403937/71‑1 (MQ=255) cTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATg < 1:3126892/71‑1 (MQ=255) tG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGAt > 1:343005/1‑69 (MQ=255) g‑AAAAAAAAAGTCCGCTTATAAGGCTTGAAAAGTTCAt < 1:2101784/38‑1 (MQ=255) aaaaaaaaaGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAg > 1:1210364/1‑70 (MQ=255) aaaaaaaaaGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAg < 1:3059355/70‑1 (MQ=255) | CGCCCTTTTTATTTCTGCAATCCGCAATTATCATCCCGCAAATCTACTATGCAATAGACCATAAACTG‑AAAAAAAAAGTCCGCTGATAAGGCTTGAAAAGTTCATTTCCAGACCCATTTTTACATCGTAGCCGATGAG > minE/2493771‑2493908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |