| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,608,096 | T→C | 7.2% | intergenic (‑206/+104) | pitA ← / ← nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,608,096 | 0 | T | C | 7.2% | 197.9 / 6.3 | 69 | intergenic (‑206/+104) | pitA/nikR | phosphate transporter, low‑affinity/DNA‑binding transcriptional regulator, Ni‑binding |
| Reads supporting (aligned to +/- strand): ref base T (41/23); new base C (3/2); total (44/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
TTCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCT > minE/2608027‑2608164 | ttCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTc > 1:2190464/1‑71 (MQ=255) aGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGt < 1:1280541/71‑1 (MQ=255) aGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGt < 1:200145/71‑1 (MQ=255) aTACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt‑t < 1:720811/71‑1 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt‑t > 1:3564136/1‑70 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt‑t > 1:4035757/1‑70 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt‑t > 1:3896372/1‑70 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtgtttt‑t > 1:1852855/1‑70 (MQ=255) tACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGttttttct < 1:1104533/71‑3 (MQ=255) aCTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGttttttct > 1:2290163/1‑68 (MQ=255) cTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑Taa < 1:2013794/70‑1 (MQ=255) cTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAag < 1:927690/71‑1 (MQ=255) cTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAag < 1:3887649/71‑1 (MQ=255) cTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAag < 1:2343505/71‑1 (MQ=255) tCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt > 1:3904238/1‑65 (MQ=255) cTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAagag > 1:1310187/1‑69 (MQ=255) cTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAagag > 1:792563/1‑69 (MQ=255) ttAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAgcg > 1:174008/1‑71 (MQ=255) tAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑Taa < 1:4091003/59‑1 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGtttttt‑t < 1:3884657/55‑1 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGttttt > 1:2265395/1‑53 (MQ=255) atatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTc > 1:204053/1‑71 (MQ=255) tatTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAgcgc < 1:152597/65‑1 (MQ=255) atTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCg > 1:1975708/1‑70 (MQ=255) ttGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:1209400/1‑71 (MQ=255) ttGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAGCGCAAcgtg < 1:2123149/70‑4 (MQ=255) gATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAgcgc > 1:1202427/1‑62 (MQ=255) gATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGCCCGTTTTTTCTAAGAGGAgcgc > 1:253055/1‑62 (MQ=255) aTGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAg > 1:365979/1‑71 (MQ=255) tGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAgg > 1:2293183/1‑71 (MQ=255) tGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAgg < 1:1394670/71‑1 (MQ=255) cTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGt < 1:3978835/71‑1 (MQ=255) cTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGt < 1:508340/71‑1 (MQ=255) ggTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGa > 1:1659849/1‑63 (MQ=255) ggTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTAt > 1:3453483/1‑71 (MQ=255) gCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAgagga > 1:2896288/1‑48 (MQ=255) cGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTa < 1:848125/70‑1 (MQ=255) aCCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑Taa > 1:3623905/1‑40 (MQ=255) ccACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTa > 1:3061273/1‑63 (MQ=255) cACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTagagag > 1:1620635/1‑71 (MQ=255) cACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTagagag < 1:3776699/71‑1 (MQ=255) aCTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAgcgc < 1:1602685/46‑1 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAag < 1:4105910/36‑1 (MQ=255) tGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGaa > 1:2911873/1‑71 (MQ=255) aGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAgaggag > 1:780990/1‑39 (MQ=255) aGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAgagg > 1:3320090/1‑37 (MQ=255) aGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAggg > 1:3285699/1‑55 (MQ=255) aGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAAt > 1:3516879/1‑70 (MQ=255) aGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATc < 1:3351166/71‑1 (MQ=255) ggAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATc < 1:3968652/70‑1 (MQ=255) gAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg > 1:862746/1‑71 (MQ=255) aaTCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAgg > 1:2564826/1‑71 (MQ=255) aTCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGgt > 1:3333432/1‑71 (MQ=255) tCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAg > 1:647437/1‑68 (MQ=255) tCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGgtg > 1:2884662/1‑71 (MQ=255) tCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGgtg > 1:546391/1‑71 (MQ=255) tCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGgt > 1:2083605/1‑70 (MQ=255) tttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCg < 1:88098/41‑1 (MQ=255) tttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGAt > 1:1375928/1‑44 (MQ=255) tttACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGtgtg > 1:3676509/1‑71 (MQ=255) tACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGtgtg < 1:1857284/69‑1 (MQ=255) tACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGc > 1:3250003/1‑70 (MQ=255) cAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCt < 1:3235412/69‑1 (MQ=255) ttCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTa < 1:96967/47‑1 (MQ=255) ttCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTgg > 1:373527/1‑71 (MQ=255) ttCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTgg > 1:2647913/1‑71 (MQ=255) aCGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGccc > 1:3366330/1‑71 (MQ=255) cGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTaga > 1:2718107/1‑45 (MQ=255) cGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCt > 1:1765890/1‑71 (MQ=255) tCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCt < 1:2468835/60‑1 (MQ=255) | TTCAGGCAAATACTTCCTTTTAGTAATATTGATGCTGGTGCGACCACTGAGGAATCTTTACAATTCACGTCCGTTTTTT‑TAAGAGGAGCGCAACTCGGATAGGGTATTTAGAGAGGAATCAGGTGTGCTAGTGGCCCT > minE/2608027‑2608164 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |