| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 848,817 | Δ1 bp | coding (407/1254 nt) | kch ← | voltage‑gated potassium channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 848,816 | 0 | G | . | 98.6% | 303.2 / ‑1.8 | 71 | coding (408/1254 nt) | kch | voltage‑gated potassium channel |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base . (36/34); minor base C (0/1); total (36/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
GTTGAGTAAAACAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGCGGCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCA > minE/848760‑848875 | gTTGAGTAAAACAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTa < 1:2150403/62‑1 (MQ=255)gTTGAGTAAAACAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTa < 1:4541958/62‑1 (MQ=255) aGTAAAACAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGt < 1:1450832/71‑1 (MQ=255) aGTAAAACAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGt < 1:1480253/71‑1 (MQ=255) gTAAAACAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGt < 1:3865839/70‑1 (MQ=255) aaaCAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGaa > 1:2406184/1‑65 (MQ=255) aaaCAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGaa > 1:4877500/1‑65 (MQ=255) aaaCAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGaa > 1:2937483/1‑65 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:768872/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:2579157/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:2019254/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:2657286/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:2838154/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:2885342/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:3448903/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:704415/1‑69 (MQ=255) tCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTAt > 1:1304594/1‑69 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATc > 1:16078/1‑69 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:3172287/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:381069/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:4116664/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:417899/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:2529401/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:2447899/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:862199/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:1385258/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:2107327/1‑71 (MQ=255) cGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAg > 1:1799040/1‑71 (MQ=255) gAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCa > 1:570982/1‑70 (MQ=255) gAAACTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCa > 1:1777568/1‑70 (MQ=255) cTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCa < 1:4462188/57‑1 (MQ=255) cTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAac < 1:51384/71‑1 (MQ=255) cTAATAAATGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAac < 1:1540039/71‑1 (MQ=255) cTAATAAATGCAAAAATTGTCCCGGCT‑CCGCGCTACTGTGGGAGAAGTCTTTGCGCa < 1:603226/57‑1 (MQ=255) aaTGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGc > 1:1104307/1‑71 (MQ=255) aaTGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGc > 1:3679155/1‑71 (MQ=255) tGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCg > 1:4411379/1‑70 (MQ=255) tGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCg > 1:244474/1‑70 (MQ=255) tGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCg > 1:735549/1‑70 (MQ=255) tGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCg > 1:1705746/1‑70 (MQ=255) tGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCg > 1:4504843/1‑70 (MQ=255) tGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCg > 1:180055/1‑70 (MQ=255) tGCAAAAATTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCg > 1:3524425/1‑70 (MQ=255) aaaaaTTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGGGCAGTATCAGCAAAAACACCAGCGTaaa < 1:4580801/71‑1 (MQ=255) aaaaaTTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTaaa < 1:2098252/71‑1 (MQ=255) aaaaaTTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTaaa < 1:462883/71‑1 (MQ=255) aaaaaTTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTaaa < 1:1828330/71‑1 (MQ=255) aaaaaTTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTaaa < 1:1960641/71‑1 (MQ=255) aaaaaTTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTaaa < 1:178074/71‑1 (MQ=255) aaaaaTTGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATAAGCAAAAACACCAGCGTaaa < 1:3060205/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:1804501/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:4347032/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:1924121/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:269029/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:1222210/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:3310340/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:4590371/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:3083567/71‑1 (MQ=255) ttGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:1967004/71‑1 (MQ=255) ctGTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGc < 1:3765359/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTaaa > 1:4686083/1‑64 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:1946273/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:847574/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:2401301/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:2465386/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:2512576/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:2869144/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:3278054/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:4482252/70‑1 (MQ=255) gTCCCGGCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCa < 1:4335573/70‑1 (MQ=255) gCTGC‑GCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTaaaaa > 1:3366026/1‑60 (MQ=255) | GTTGAGTAAAACAGTAATGTCGTGAAACTAATAAATGCAAAAATTGTCCCGGCTGCGGCGCTACTGTGGGAGAAGTCTTTGCGCAGTATCAGCAAAAACACCAGCGTAAAAATGCA > minE/848760‑848875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |