BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,411,263	A→C	7.6%	A30A (GCT→GCG)	yfbS ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,411,263	0	A	C	7.6%	2889.9 / 43.6	994	A30A (GCT→GCG)	yfbS	putative transporter
Reads supporting (aligned to +/- strand): ref base A (593/297); new base C (2/101); total (596/398)
Fisher's exact test for biased strand distribution p-value = 3.26e-41
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1057 total aligned reads displayed.

CCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA  >  NC_000913/2411114‑2411411

  cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGc                                                                                                                                                    >  1:6224212/1‑150 (MQ=255)

  cATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGc                                                                                                                                                    <  2:7726126/150‑1 (MQ=255)

   aTTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGCCACTAACAAACAAAGCg                                                                                                                                                   >  1:713943/1‑150 (MQ=255)

    ttACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGa                                                                                                                                                  <  2:3933416/150‑1 (MQ=255)

       cGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCg                                                                                                                                               >  1:2834467/1‑150 (MQ=255)

         gTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCa                                                                                                                                             >  2:2375109/1‑150 (MQ=255)

             cAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCa                                                                                                                                         >  1:10157488/1‑150 (MQ=255)

              aaCACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCAt                                                                                                                                        >  1:6557701/1‑150 (MQ=255)

               acacCTGTACGGACAAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGAAAACGTTAGGATCATAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGAAAACGCGACAATAACAAACAAAGCGACCGCATCCATa                                                                                                                                       <  2:8266615/150‑1 (MQ=255)

               acacCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGTACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATa                                                                                                                                       <  2:2742156/150‑1 (MQ=255)

               acacCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATa                                                                                                                                       >  2:1538145/1‑150 (MQ=255)

               acacCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATa                                                                                                                                       >  2:655451/1‑150 (MQ=255)

                ccccGGTACGGACCAAACCAGCGCCAATAATAAACAAGGCGGCAATCAGGACAAGGTTAGGATCAGAAAAGCCGGAAAATAGTTCTGGGACTGTCTGCTTTCCG‑ATTACTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATAc                                                                                                                                      <  2:7915855/148‑1 (MQ=255)

                   cGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCa                                                                                                                                   >  1:5387222/1‑150 (MQ=255)

                   cGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCa                                                                                                                                   >  2:1967029/1‑150 (MQ=255)

                   cGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCa                                                                                                                                   >  2:6131363/1‑150 (MQ=255)

                    ggTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCAc                                                                                                                                  >  1:5867831/1‑150 (MQ=255)

                      tACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCActc                                                                                                                                >  1:1335717/1‑150 (MQ=255)

                         gtaCCAAACATTCGCAAATATTAAACATGGCGTCATTAGGGAGATCTTTAGTTTCAAAATAGCGGTAAAATATTTCTGGGAGTTTCAGC‑TTCGGTTTTACTGCAACCTCTACAATAACAAACAACGCGACCGCATCCATACGCACTCTGc                                                                                                                             <  2:4127730/148‑1 (MQ=255)

                          gACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGcc                                                                                                                            >  1:11096791/1‑150 (MQ=255)

                           aCCAAACCATCGCCAATAATACACACGGCGGCATTCCGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTACTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                                           <  2:9059061/150‑1 (MQ=255)

                           aCCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCACACGCGACAATAACACACAAAGCGACCGCATCCATACGCACTCTGccc                                                                                                                           >  1:9945726/1‑150 (MQ=255)

                                  cATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCaa                                                                                                                    >  2:4476304/1‑150 (MQ=255)

                                        aatttataaACCCGGCGGAAATCTGCACACCTTTCGGATCATAACAGGCGGAAAATACTTGGGGTACTGTCCGGGTTCGG‑CTTATGGCAAACGCTAAAATCACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGa                                                                                                              <  2:970544/145‑1 (MQ=255)

                                        aaataatAAACATGGCGGCAATCAGGACAACTTTTGGATCATAAAAGCCGGAAAATACTTCTGGTACTGTCAGCGTTCCG‑CTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGa                                                                                                              <  2:7061287/149‑1 (MQ=255)

                                         aataatAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGAc                                                                                                             >  2:4810104/1‑150 (MQ=255)

                                           taataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGa                                                                                                           >  1:4927400/1‑150 (MQ=255)

                                             ataaACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATg                                                                                                         >  1:6076281/1‑150 (MQ=255)

                                               aaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGc                                                                                                       >  2:1652183/1‑150 (MQ=255)

                                               aaaCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGc                                                                                                       >  1:21768/1‑150 (MQ=255)

                                                 aCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCaa                                                                                                     >  2:10406110/1‑150 (MQ=255)

                                                 aCAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCaa                                                                                                     >  1:7671056/1‑150 (MQ=255)

                                                      gcggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGcc                                                                                                >  2:11123131/1‑150 (MQ=255)

                                                        ggcAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAg                                                                                              >  2:6277799/1‑150 (MQ=255)

                                                            aTCTGGACAAGGTTAGTATCAGAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAAt                                                                                          <  2:8922118/150‑1 (MQ=255)

                                                             acAGGACAACTTTAGCAAAACAAAAGCCGTAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATCAAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATg                                                                                         <  2:9901473/149‑1 (MQ=255)

                                                              cAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGa                                                                                        >  1:5277623/1‑150 (MQ=255)

                                                                ggACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGaaa                                                                                      <  2:9317020/150‑1 (MQ=255)

                                                                  aCAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGa                                                                                    >  2:426184/1‑150 (MQ=255)

                                                                      ctttAGCACCAAAAAAGCCGCAAAATAATACTGTGCCTGTCCGCGTTCCT‑ATTAATCAAAACGCGAAAAAAAAAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAAccc                                                                                <  1:5685232/148‑1 (MQ=255)

                                                                       gTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCa                                                                               >  2:3807195/1‑150 (MQ=255)

                                                                        tttggATCATAAACGCCGTAAAATACTTCTGGGACTTTCAGGTTTCCG‑CTTAATGAAAACGCGACAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                                                                              <  2:3411767/147‑1 (MQ=255)

                                                                        ttAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCaa                                                                              >  1:784609/1‑150 (MQ=255)

                                                                          tttataaGAAAAGCCTTAAAATACTTCTGGGACGGTCAGCGTTCCT‑ATTAATGAAAACGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAt                                                                            <  2:4946714/144‑1 (MQ=255)

                                                                            gATCATAAACGCCGTAAAATACTTCTGGGAGTGTCTGGTTTCCG‑ATTACTGAAAACGCTAAAATAAAAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                          <  1:927895/150‑1 (MQ=255)

                                                                            gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                          >  2:7527543/1‑150 (MQ=255)

                                                                            gATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCa                                                                          >  2:10343440/1‑150 (MQ=255)

                                                                              tCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAt                                                                        >  1:4737486/1‑150 (MQ=255)

                                                                               cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                       >  2:3291585/1‑150 (MQ=255)

                                                                               cAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAAtt                                                                       >  1:3360296/1‑150 (MQ=255)

                                                                                aGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTc                                                                      >  2:10811439/1‑150 (MQ=255)

                                                                                 gAAAAGCCGTAAATTACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                     <  2:1831568/150‑1 (MQ=255)

                                                                                 gAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCa                                                                     >  1:5124122/1‑150 (MQ=255)

                                                                                   aaaGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCAcc                                                                   >  1:1782901/1‑150 (MQ=255)

                                                                                       ccGGAAAATACTTCTGGGACTGTCAGCGATCCG‑CTTAATGCAAACGCGACCATAACAAACAAAGCGACCGCATCCATAAGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGAAATGAAAGAACCCAAATCAAATCACCGTTc                                                               >  1:3027404/1‑150 (MQ=255)

                                                                                        cgtaaaaTTCTTCTGGTAGTGTCAGGGTTCCT‑ATTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTca                                                              <  1:2543706/147‑1 (MQ=255)

                                                                                         ggAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcac                                                             >  1:10411827/1‑150 (MQ=255)

                                                                                          gAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTcaca                                                            >  2:5914200/1‑150 (MQ=255)

                                                                                           aaaaTACTTCTGGTACTGTCAGCGTTCCG‑ATTAATCAAAACGCGAAAATAACAAAAAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACaa                                                           <  1:10709843/150‑1 (MQ=255)

                                                                                                 cTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATc                                                     <  2:3540697/150‑1 (MQ=255)

                                                                                                 cTTCTGGGACTGTCAGCGTTCCG‑CTTAATGAAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATc                                                     <  1:8370741/150‑1 (MQ=255)

                                                                                                 cTTCTGGGACGGTCAGCTTTCCG‑CTAAATGCAAACCCCACAATAACAACCAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTacc                                                     <  1:3855315/150‑3 (MQ=255)

                                                                                                    cTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                                                  >  2:8621081/1‑150 (MQ=255)

                                                                                                    cTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                                                  >  2:10124069/1‑150 (MQ=255)

                                                                                                    cTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCAACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                                                  >  2:2519931/1‑150 (MQ=255)

                                                                                                    cTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCtt                                                  <  1:10011370/150‑1 (MQ=255)

                                                                                                      gggACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGt                                                <  2:3222948/150‑1 (MQ=255)

                                                                                                       ggcccgccAGCGTTCCC‑CTCCCTCCAACCGCGACACCCACACCCCACGCGACCGCATCCATCCGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCACATCGATTCACCGTTCACAACTTATCCTTGtt                                               <  2:6826343/143‑1 (MQ=255)

                                                                                                       ggACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGtt                                               >  1:2318492/1‑150 (MQ=255)

                                                                                                             tCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGa                                         >  2:5148693/1‑150 (MQ=255)

                                                                                                              ctgctttCCG‑ATTAATGCAAACGCTAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAg                                        <  2:8040290/145‑1 (MQ=255)

                                                                                                              ccgCGGTCCG‑CTTAATGCAACCGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAg                                        <  2:8806497/148‑1 (MQ=255)

                                                                                                               aGCGTTCCG‑ATTAATGCAAACGCTACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAgg                                       <  1:1643709/150‑1 (MQ=255)

                                                                                                                gCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg                                      >  1:806128/1‑150 (MQ=255)

                                                                                                                gCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAggg                                      <  1:6484978/150‑1 (MQ=255)

                                                                                                                   ttCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGa                                   >  1:4213965/1‑150 (MQ=255)

                                                                                                                   ttCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGa                                   >  1:404409/1‑150 (MQ=255)

                                                                                                                         cTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTCATTGAGGGGGAGGACt                              >  2:8423476/1‑150 (MQ=255)

                                                                                                                         cTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                              >  1:3607102/1‑150 (MQ=255)

                                                                                                                         cTTAATGCAAACGCGAAAATAAAAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACt                              <  2:7147095/150‑1 (MQ=255)

                                                                                                                            aaTGCAACCGCCAAAATAACAACCAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                           <  1:1055450/150‑1 (MQ=255)

                                                                                                                            aaTGCAAACGCGACAATAACAAACAACGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGa                           <  2:5677468/150‑1 (MQ=255)

                                                                                                                             aTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTggt                          <  2:10620149/150‑3 (MQ=255)

                                                                                                                             aTGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAt                          >  1:8151145/1‑150 (MQ=255)

                                                                                                                              tGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAtt                         >  2:2173556/1‑150 (MQ=255)

                                                                                                                               gCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTc                        <  2:739833/150‑1 (MQ=255)

                                                                                                                                 aaaCGCGAAAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTg                      <  1:8712934/150‑1 (MQ=255)

                                                                                                                                    cgcgACAATACCAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCa                   <  1:7366664/150‑1 (MQ=255)

                                                                                                                                       gACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaa                <  1:6886768/150‑1 (MQ=255)

                                                                                                                                          aaTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa             >  2:5282809/1‑150 (MQ=255)

                                                                                                                                          aaTAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaa             >  2:554737/1‑150 (MQ=255)

                                                                                                                                           aaaaaaaacaacGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATaaaaaa            <  2:2861332/145‑1 (MQ=255)

                                                                                                                                             aacaaacaCAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGc          <  2:11015732/150‑1 (MQ=255)

                                                                                                                                             aacaaacaAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGc          >  2:951194/1‑150 (MQ=255)

                                                                                                                                              acaaacaaAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcc         >  2:6868679/1‑150 (MQ=255)

                                                                                                                                                aaacaacGCGACCGCATCCATACGCACTCGGCCCGTCGCAAACACGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGcccc       <  1:9776175/150‑1 (MQ=255)

                                                                                                                                                 aacaaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcg      >  1:94780/1‑150 (MQ=255)

                                                                                                                                                  acaaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCTTTCACAACTTATCCTTGTTATTTGTGGGGGATGACTTGATTCTGCAATAAAAAAGCCCcga     <  1:9054972/150‑1 (MQ=255)

                                                                                                                                                   caacGCGACCGAATCAATAGGCACTCTGCCCGTCGAAAACAATAGGATGGCAAGGGCCTTTAATGAAATAACCCAAATCAATTCACCTTTCACAAATTATCCTTTTTATTTGTGGGGTATGACTTTATTCTGCCATAAAAAAGCCCcgac    <  1:4536010/150‑1 (MQ=255)

                                                                                                                                                   caaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac    >  2:1309734/1‑150 (MQ=255)

CCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGTTAGGATCAGAAAAGCCGGAAAATACTTCTGGGACTGTCAGCGTTCCG‑CTTAATGCAAACGCGACAATAACAAACAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGA  >  NC_000913/2411114‑2411411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`