Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,019,460	G→A	100%	V359I (GTC→ATC)	fliK →	flagellar hook‑length control protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,019,460	0	G	A	100.0%	243.6 / NA	73	V359I (GTC→ATC)	fliK	flagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/47);  total (26/47)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGAT  >  NC_000913/2019324‑2019586                                                                                                                                          |                                                                                                                               tCAGTTAGGGCACAGCATCATCAGTGGCGAAAGCTTTATTGGTCAGCAGCAGGCAGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTATGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                             <  2:724371/140‑1 (MQ=255) tCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                             <  2:316350/140‑1 (MQ=255) tCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                             <  2:879296/140‑1 (MQ=255) tCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                             <  2:1198789/140‑1 (MQ=255) tCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                             <  2:1224590/140‑1 (MQ=255)       aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                       >  1:447854/1‑140 (MQ=255)       aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                       >  1:1648823/1‑140 (MQ=255)       aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTAc                                                                                                                       >  1:1648238/1‑140 (MQ=255)        gggCAAAGCAACATCTGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGACGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                      <  1:596644/140‑1 (MQ=255)        gggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                      <  1:594343/140‑1 (MQ=255)        gggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACa                                                                                                                      <  1:594486/140‑1 (MQ=255)         ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCCCCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCGGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACaa                                                                                                                     <  1:480267/140‑1 (MQ=255)         ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGTCGACGATACGCTTCCGGTTCCCATCTCTTTACaa                                                                                                                     <  2:1687528/140‑1 (MQ=255)              aGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                <  2:26537/140‑1 (MQ=255)              aGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                >  2:1360597/1‑140 (MQ=255)              aGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                <  2:515647/140‑1 (MQ=255)              aGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCg                                                                                                                >  2:1357536/1‑140 (MQ=255)                cAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                              <  2:1241746/140‑1 (MQ=255)                cAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                              <  2:208640/140‑1 (MQ=255)                cAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                              <  2:208779/140‑1 (MQ=255)                  aCATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTa                                                                                                            <  1:759871/140‑1 (MQ=255)                   cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                           <  1:987519/140‑1 (MQ=255)                    aTCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTaa                                                                                                           <  1:441105/140‑1 (MQ=255)                       aGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAgg                                                                                                       >  1:1257205/1‑140 (MQ=255)                        gTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                      >  1:710195/1‑140 (MQ=255)                        gTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                      >  2:1292297/1‑140 (MQ=255)                        gTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                      >  2:1264061/1‑140 (MQ=255)                        gTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGc                                                                                                      >  2:204115/1‑140 (MQ=255)                          ggCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCaa                                                                                                    >  2:1182443/1‑140 (MQ=255)                           gCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAAc                                                                                                   >  2:1611477/1‑140 (MQ=255)                             gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                 <  2:1599825/140‑1 (MQ=255)                             gAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACATTGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAg                                                                                                 <  1:493752/140‑1 (MQ=255)                                     tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTg                                                                                         >  1:29065/1‑140 (MQ=255)                                     tAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTg                                                                                         >  1:650213/1‑140 (MQ=255)                                          gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtt                                                                                    <  2:386309/140‑1 (MQ=255)                                            cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                                                                  >  1:1294297/1‑140 (MQ=255)                                            cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                                                                  >  1:1416802/1‑140 (MQ=255)                                            cagcagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                                                                  >  1:211077/1‑140 (MQ=255)                                                agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGCACCTCTGGC‑GGGGGAAGACGAAGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGcc                                                                              <  2:89731/140‑1 (MQ=255)                                                 gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCt                                                                             <  2:1274437/140‑1 (MQ=255)                                                 gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCt                                                                             <  2:281813/140‑1 (MQ=255)                                                  cagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTa                                                                            >  1:632863/1‑140 (MQ=255)                                                       cGCTTCCCCGCCACAGCACAGCCAACGCACAGCAAACCACGAACCACTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACACGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                                                                       <  2:1648699/140‑1 (MQ=255)                                                       cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                                                                       <  1:28043/140‑1 (MQ=255)                                                       cGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTc                                                                       <  1:1169692/140‑1 (MQ=255)                                                               agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg                                                               <  1:266734/140‑1 (MQ=255)                                                               agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAg                                                               <  1:110838/140‑1 (MQ=255)                                                               agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGACAGAGGTAg                                                               <  1:1037535/140‑1 (MQ=255)                                                                 caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCa                                                             >  2:1498527/1‑140 (MQ=255)                                                                   acagcaaATCCATCGAACAGCAAACCATGATCCTCTTGC‑GGGGGAAGACGACGCTTCGCTTCCGGTTCCCATCTCTTTACAAGGTCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc                                                           <  2:319929/140‑1 (MQ=255)                                                                   aaagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGAAGACGATACGCTTCAGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCATAGGTAGCTcc                                                           <  2:655640/138‑1 (MQ=255)                                                                           gCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcg                                                   <  1:1515396/140‑1 (MQ=255)                                                                           gCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcg                                                   <  1:1515534/140‑1 (MQ=255)                                                                            ccAACGCACAGCAAACCATGAACCTCGGGC‑GGGGGAAGACGACGATACGCTTCCGGATCCCATCTGTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGCGGTAGCTCCGTAATCcgc                                                  >  2:959204/1‑140 (MQ=255)                                                                             cAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgcg                                                 <  2:70730/140‑1 (MQ=255)                                                                                     cgCAAACCATGACCCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACCAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc                                         <  2:1290459/139‑1 (MQ=255)                                                                                     aGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc                                         >  1:1025814/1‑140 (MQ=255)                                                                                     aGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTcc                                         <  1:1462693/140‑1 (MQ=255)                                                                                                     ctGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTc                         >  1:1144670/1‑140 (MQ=255)                                                                                                     ctGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTc                         >  1:568170/1‑140 (MQ=255)                                                                                                      tGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCa                        >  1:1461211/1‑140 (MQ=255)                                                                                                            ggggAAGACGACGATACGCTTCCGGTTCCCATCCCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACg                   <  1:1019741/140‑1 (MQ=255)                                                                                                              ggAAGTCGACGCTACGCTTCCGGTTCCCATCTCTATACAAGGGCGTGTAACAGGCATCAGCGGCGTAGATATAAACGCCAAACGTCAGAGGTAGCACCGTAATCAGCGTCTTTTCCCAGCCCTGTTGCGCTCAAGACGCa                 <  2:1242189/140‑1 (MQ=255)                                                                                                              ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                 <  1:1528286/140‑1 (MQ=255)                                                                                                              ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                 <  2:806052/140‑1 (MQ=255)                                                                                                              ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                 >  1:1278157/1‑140 (MQ=255)                                                                                                              ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                 >  1:608067/1‑140 (MQ=255)                                                                                                              ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCa                 <  2:169563/140‑1 (MQ=255)                                                                                                                aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAgg               <  2:926163/140‑1 (MQ=255)                                                                                                                        gaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAg       <  1:1596543/140‑1 (MQ=255)                                                                                                                         aTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc      <  2:169738/140‑1 (MQ=255)                                                                                                                             gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGAt  <  2:227472/140‑1 (MQ=255)                                                                                                                             gCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGAt  <  2:227386/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                               TCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGC‑GGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGAT  >  NC_000913/2019324‑2019586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑