Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	222,958	Δ1 bp	100%	coding (126/576 nt)	gmhB →	D,D‑heptose 1,7‑bisphosphate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	222,958	0	A	.	100.0%	138.2 / NA	29	coding (126/576 nt)	gmhB	D,D‑heptose 1,7‑bisphosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (18/11);  total (18/11)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCTATT  >  NC_000913/222817‑223103                                                                                                                                              |                                                                                                                                                  taAGGAGATATAAAAGGTGGCGAAGACCGTACCCGCAAATTATCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTaaaaaaatg                                                                                                                                           <  2:34772/150‑3 (MQ=255)     gAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCt                                                                                                                                      >  2:141808/1‑150 (MQ=255)       gCTATAAAAGGTGGCGAAGAGCATACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCttt                                                                                                                                    >  2:112555/1‑150 (MQ=255)       gCTATAAAAGGTGGCGAAGAGCATACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCttt                                                                                                                                    >  2:229231/1‑150 (MQ=255)           taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCGATGAGATCGACAACTTTGAATTTATCGAGGGTGTTATTGAAGCCATGCGCGAGCT‑AAAAAATTGGGCTTTgcgc                                                                                                                                >  2:38972/1‑150 (MQ=255)           taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTgcgc                                                                                                                                >  2:111132/1‑150 (MQ=255)           taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTgcgc                                                                                                                                >  2:111131/1‑150 (MQ=255)           taAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTgcgc                                                                                                                                >  2:36156/1‑150 (MQ=255)                   ggCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGgta                                                                                                                        <  2:195455/150‑1 (MQ=255)                    gCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGgtag                                                                                                                       >  2:217665/1‑150 (MQ=255)                                 ccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAATAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTc                                                                                                          >  1:161849/1‑150 (MQ=255)                                 ccGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAgac                                                                                                          >  1:215606/1‑148 (MQ=255)                                           tCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCt                                                                                                >  2:61030/1‑150 (MQ=255)                                               gACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcgcg                                                                                            <  1:37082/150‑1 (MQ=255)                                               gACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTcgcg                                                                                            <  1:37083/150‑1 (MQ=255)                                                aCCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCgg                                                                                           <  1:127639/150‑1 (MQ=255)                                                     gATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAAt                                                                                      >  2:78034/1‑150 (MQ=255)                                                                  aTGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGcaca                                                                         <  2:213079/150‑1 (MQ=255)                                                                                tATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGa                                                           <  2:192107/150‑1 (MQ=255)                                                                                           gaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCTCAGTTTGAAACGCTGACCGAGtggatg                                                >  1:209512/1‑150 (MQ=255)                                                                                           gaTCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGtggatg                                                >  1:8090/1‑150 (MQ=255)                                                                                               gACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACt                                            >  1:115881/1‑150 (MQ=255)                                                                                                aCAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTg                                           >  2:203834/1‑150 (MQ=255)                                                                                                aCAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTg                                           <  1:203834/150‑1 (MQ=255)                                                                                                      ttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCt                                     >  1:209506/1‑150 (MQ=255)                                                                                                      ttGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCt                                     >  1:119042/1‑150 (MQ=255)                                                                                                          aTTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCg                                 <  1:229231/150‑1 (MQ=255)                                                                                                          aTTTATCGACGGTGTTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCg                                 <  1:112555/150‑1 (MQ=255)                                                                                                                      tgtTATTGACGCCATGCGCGAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTc                     <  1:181548/150‑1 (MQ=255)                                                                                                                                         gAGCT‑AAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCtatt  <  2:242166/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                  TAAGGAGCTATAAAAGGTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGACAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCAACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCGGACCGAGATGTCGATCTGGATGGTATCTATT  >  NC_000913/222817‑223103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑