BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,821,424	G→C	88.3%	G196G (GGC→GGG)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,821,424	0	G	C	88.3%	317.0 / 32.5	94	G196G (GGC→GGG)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (6/5); new base C (41/42); total (47/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

Only 100 of 102 total aligned reads displayed.

TCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTC  >  NC_000913/2821287‑2821558

tCCAGACCCCTACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAAGGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  2:16717/1‑140 (MQ=255)

tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCCTCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  2:17737/1‑140 (MQ=255)

tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  2:1873131/1‑140 (MQ=255)

tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  2:101717/1‑140 (MQ=255)

tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  1:563833/1‑140 (MQ=255)

tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  1:909386/1‑140 (MQ=255)

tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  2:864331/1‑140 (MQ=255)

tCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAAAGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑ccc                                                                                                                                      >  1:336820/1‑140 (MQ=255)

  cAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑Ccccc                                                                                                                                    <  1:1225530/140‑1 (MQ=255)

  cAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑Ccccc                                                                                                                                    >  1:1321444/1‑140 (MQ=255)

  cAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑Ccccc                                                                                                                                    >  2:582287/1‑140 (MQ=255)

        cATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCaaa                                                                                                                              >  2:576243/1‑140 (MQ=255)

        cATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCaaa                                                                                                                              >  2:576628/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTATCGGTCGCCGTCTTCTTCAGGGCTTCCCGGAGG‑CCACCCCCAAAtg                                                                                                                            >  1:1465313/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  1:617333/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:1484066/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:180143/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:1377721/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:960483/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:461893/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:170537/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  1:4794/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:1891498/1‑140 (MQ=255)

          tACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAAtg                                                                                                                            >  2:1758442/1‑140 (MQ=255)

               gTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTc                                                                                                                       >  2:254891/1‑140 (MQ=255)

                 aTCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGc                                                                                                                     >  2:892872/1‑140 (MQ=255)

                  tCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGcc                                                                                                                    <  1:108001/140‑1 (MQ=255)

                    tACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGt                                                                                                                  <  2:1100918/140‑1 (MQ=255)

                    tACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGt                                                                                                                  <  2:1100671/140‑1 (MQ=255)

                     aCAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTg                                                                                                                 >  1:1037320/1‑140 (MQ=255)

                     aCAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTg                                                                                                                 >  1:1037373/1‑140 (MQ=255)

                         aCGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCg                                                                                                             >  1:723421/1‑140 (MQ=255)

                         aCGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCg                                                                                                             >  1:1636930/1‑140 (MQ=255)

                         aCGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCg                                                                                                             >  2:1341818/1‑140 (MQ=255)

                          cGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGt                                                                                                            >  1:70439/1‑140 (MQ=255)

                          cGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGt                                                                                                            >  1:70369/1‑140 (MQ=255)

                              ttCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCCAAATGTGGTCGCCGTGATCGTaga                                                                                                         >  1:1091474/1‑140 (MQ=255)

                              ttCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTagaa                                                                                                        >  2:317125/1‑140 (MQ=255)

                              ttCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTagaa                                                                                                        >  2:317222/1‑140 (MQ=255)

                               tCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTagaag                                                                                                       >  2:1381307/1‑140 (MQ=255)

                               tCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTagaag                                                                                                       >  2:1381312/1‑140 (MQ=255)

                                   cAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGAttt                                                                                                   <  2:661801/140‑1 (MQ=255)

                                          tCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCa                                                                                            <  1:1176517/140‑1 (MQ=255)

                                              tCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACggg                                                                                        <  1:832904/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAACTGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                   <  1:1267455/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGgcagca                                                                                   <  2:1809085/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCTCa                                                                                   <  1:423185/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                   <  1:268078/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                   <  1:1070617/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                   <  1:724786/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                   <  1:423321/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                   <  1:180143/140‑1 (MQ=255)

                                                   ccATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAAAGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTCGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCa                                                                                   <  2:909386/140‑1 (MQ=255)

                                                       cGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGa                                                                               <  1:648898/140‑1 (MQ=255)

                                                        ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCACATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                              <  1:1531083/140‑1 (MQ=255)

                                                        ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                              <  1:1406706/140‑1 (MQ=255)

                                                        ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCAGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                              <  1:668051/140‑1 (MQ=255)

                                                        ggCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCAGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAc                                                                              <  1:667907/140‑1 (MQ=255)

                                                         gCCTTGCGGTTGAACTGCATGAAGAAGATGTTCCATATCTCAATGTTTCGGTAGCCGTCTTCTTCCGGGCTTCCCGGTGGCCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCATGGAc                                                                              <  1:805076/140‑1 (MQ=255)

                                                         gCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAcc                                                                             <  2:562349/140‑1 (MQ=255)

                                                          ccTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGGCCCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGAcc                                                                             <  2:547584/140‑1 (MQ=255)

                                                           cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                                                                           <  1:229064/140‑1 (MQ=255)

                                                           cTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAg                                                                           <  1:228821/140‑1 (MQ=255)

                                                              gCGGTTGAACTGCATGAAGACGATGTTCCAGAACTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAgtgt                                                                        <  1:1510996/140‑1 (MQ=255)

                                                                    gAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCa                                                                  <  1:1710618/140‑1 (MQ=255)

                                                                    gAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGAGTCACCCa                                                                  >  1:1661842/1‑140 (MQ=255)

                                                                        tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTg                                                              <  1:619646/140‑1 (MQ=255)

                                                                        tGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTg                                                              <  1:532728/140‑1 (MQ=255)

                                                                          cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                                                            >  1:956016/1‑140 (MQ=255)

                                                                          cATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGcc                                                            >  2:1672306/1‑140 (MQ=255)

                                                                           aTGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCa                                                           <  1:1343574/140‑1 (MQ=255)

                                                                            tGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAg                                                          >  2:463880/1‑140 (MQ=255)

                                                                              aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa                                                        <  2:70439/140‑1 (MQ=255)

                                                                              aaGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGaa                                                        <  2:70369/140‑1 (MQ=255)

                                                                                    aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCGCGGAGG‑CCCCCCCCAAATGGGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGTGCCGTGGCACCCATCTGCCAGAAGTGGtg                                                  >  2:486477/1‑139 (MQ=255)

                                                                                    aTGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTc                                                  >  1:1104276/1‑140 (MQ=255)

                                                                                      gTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAg                                                <  2:563833/140‑1 (MQ=255)

                                                                                       ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa                                               <  1:1698394/140‑1 (MQ=255)

                                                                                       ttCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGa                                               <  1:1698152/140‑1 (MQ=255)

                                                                                        tCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCGCGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGAt                                              <  1:62573/140‑1 (MQ=255)

                                                                                           aGATCTCACTGTATCGGTCGCCGTCTTCTTCGGGGCTTCCCGGAGG‑CCCCCCCCACATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCTGTGTCACCCATATGCCAGAAGTTGTCTGATGCg                                           <  2:969061/140‑1 (MQ=255)

                                                                                             aTCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTa                                         <  1:1865400/140‑1 (MQ=255)

                                                                                               ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATg                                       >  1:17632/1‑140 (MQ=255)

                                                                                               ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATg                                       >  1:922692/1‑140 (MQ=255)

                                                                                               ctcAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATg                                       >  2:1641169/1‑140 (MQ=255)

                                                                                                  aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgcg                                    <  1:1400055/140‑1 (MQ=255)

                                                                                                  aaTGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGgcg                                    >  1:1815011/1‑140 (MQ=255)

                                                                                                       aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCt                               >  2:545095/1‑140 (MQ=255)

                                                                                                       aGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCt                               <  1:1672306/140‑1 (MQ=255)

                                                                                                            tCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt                          >  1:1843335/1‑140 (MQ=255)

                                                                                                            tCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt                          >  1:1108215/1‑140 (MQ=255)

                                                                                                            tCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt                          >  2:1635311/1‑140 (MQ=255)

                                                                                                            tCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGtt                          >  2:594170/1‑140 (MQ=255)

                                                                                                            tCGCCGTCTTCTTCCGGGCTTCCCGGA‑G‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTa                         >  2:1163988/1‑140 (MQ=255)

                                                                                                                  tcttctTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGcc                    <  2:352401/140‑1 (MQ=255)

                                                                                                                  tcttctTCCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGcc                    <  2:352815/140‑1 (MQ=255)

                                                                                                                     tcttcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGAt                 <  1:1106581/140‑1 (MQ=255)

                                                                                                                        tcCGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCg              <  1:58148/140‑1 (MQ=255)

                                                                                                                          cGGGCTTCCCGGAGG‑CCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTACGGTGCACGGGCCGCACGGAACAGTGTCACCCATCTGCCAGAAGTTGTCATATGCGTATGGCGCACCTTTGTTATCGCCGATGCGaa            <  2:1354961/140‑1 (MQ=255)

                                                                                                                                    ggAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTc  <  1:1747880/140‑1 (MQ=255)

TCCAGACCCATACCGGTATCTACAGACGGCTTCGGCAGCGGTTCCATCGTGCCATCGGCCTGGCGGTTGAACTGCATGAAGACGATGTTCCAGATCTCAATGTAGCGGTCGCCGTCTTCTTCCGGGCTTCCCGGAGG‑GCCCCCCCAAATGTGGTCGCCGTGATCGTAGAAGATTTCGGTGCACGGGCCGCACGGACCAGTGTCACCCATCTGCCAGAAGTTGTCAGATGCGTATGGCGCACCTTTGTTATCGCCGATGCGAATAATACGTTC  >  NC_000913/2821287‑2821558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`