Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,982,684	(C)8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,982,676	1	.	C	94.4%	326.5 / 13.6	89	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base . (3/2);  new base C (51/33);  total (54/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982544‑3982809                                                                                                                                      |                                                                                                                                       tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccc                                                                                                                                >  1:1040498/1‑140 (MQ=255) tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccc                                                                                                                                >  1:1231804/1‑140 (MQ=255) tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccc                                                                                                                                >  1:1009666/1‑140 (MQ=255) tgATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGGTAGTGTCCTTACGGTCGTGGGGGGTCAAGT‑‑ccccccc                                                                                                                                >  1:1040707/1‑140 (MQ=255)      ccAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccccctcg                                                                                                                           >  1:1711100/1‑136 (MQ=255)      ccAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccccctcg                                                                                                                           >  2:156695/1‑136 (MQ=255)      ccAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATGAGAAGTTGTGACAATGAGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccccctcg                                                                                                                           >  2:157804/1‑136 (MQ=255)      ccAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATAATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACTCGCTAGATTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccccctcg                                                                                                                           >  2:156711/1‑136 (MQ=255)      ccAGTTGTCCTGCGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCAAGCTACAGGTGTTAGAGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccccccac                                                                                                                           >  2:952430/1‑136 (MQ=21)       cAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑ccccccccctcgc                                                                                                                          >  2:1340532/1‑135 (MQ=255)       cAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTACTTACGGACGTGGGGGTTCAAGT‑‑cccccccccacgc                                                                                                                          >  2:1159467/1‑135 (MQ=255)         gTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGcac                                                                                                                         >  2:1235440/1‑140 (MQ=255)         gTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGcac                                                                                                                         >  2:866018/1‑140 (MQ=255)           tGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGcacca                                                                                                                       <  1:1363520/140‑1 (MQ=255)           tGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGcacca                                                                                                                       <  2:289547/140‑1 (MQ=255)             tCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACg                                                                                                                     >  2:335552/1‑140 (MQ=255)             tCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACg                                                                                                                     >  2:334660/1‑140 (MQ=255)               gTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGAc                                                                                                                   >  2:1514941/1‑140 (MQ=255)               gTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGAc                                                                                                                   >  2:1515922/1‑140 (MQ=255)                       gAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTCCCCCCCCCCTCGCACCACGACTTTaaat                                                                                                            >  2:681183/1‑139 (MQ=255)                       gAATCCCATAAGCCACACCATTATTAGAAGTTGTGACAATGATAAGGTGGCGGAATTGGAAGACGCGCTAGCATAAGGTGATAGTGTCCTTACGGACGTGTTGGTTCAATTC‑CCCCCCCCACTCACCACCACTTTAAAGa                                                                                                           >  2:196402/1‑140 (MQ=18)                            ccATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGa                                                                                                      >  2:1022769/1‑140 (MQ=255)                            ccATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGa                                                                                                      >  2:1386043/1‑140 (MQ=255)                              aTTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACGTTAAAGAATTTAAc                                                                                                    >  2:1248994/1‑140 (MQ=255)                              aTTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCACGCACCACGACTTTAAAGAATTGAAc                                                                                                    >  2:1248590/1‑140 (MQ=255)                                  gCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGTTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTAACGGACGTGGGGGTTCAAGTC‑CCCCCCCCACGCACCACGACTATAAAGAATAGAACTaaa                                                                                                >  1:1671172/1‑140 (MQ=21)                                   ccACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTTAACTaaaa                                                                                               >  1:214776/1‑140 (MQ=255)                                       cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTc                                                                                           >  1:394204/1‑140 (MQ=255)                                       cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTc                                                                                           >  1:1266170/1‑140 (MQ=255)                                       cccATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATAGAACTAAAAATTc                                                                                           >  1:606412/1‑140 (MQ=255)                                          attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                                                                       <  1:1223133/140‑1 (MQ=255)                                          attattAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                                                                       <  1:1224739/140‑1 (MQ=255)                                           ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                                                                      >  2:1643416/1‑140 (MQ=255)                                            tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                                                                      >  2:546614/1‑140 (MQ=255)                                             attaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                                                                     <  1:1103505/140‑1 (MQ=255)                                               taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                                                                   <  1:552139/140‑1 (MQ=255)                                               taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCa                                                                                   <  1:1429656/140‑1 (MQ=255)                                                 gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                                                                 >  2:1262000/1‑140 (MQ=255)                                                 gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                                                                >  1:1703690/1‑140 (MQ=255)                                                  aaGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                                                               >  2:234587/1‑140 (MQ=255)                                                   aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                                                               >  2:733681/1‑140 (MQ=255)                                                   aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                                                               >  2:733802/1‑140 (MQ=255)                                                    gTTGTGACAATGCGAAGGTGGCGTAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                                                              >  1:658697/1‑140 (MQ=255)                                                      tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  2:14551/1‑140 (MQ=255)                                                      tgtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                                                            >  2:935273/1‑140 (MQ=255)                                                       gtgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACTACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                                                           >  2:422425/1‑140 (MQ=255)                                                       gtgAAAATGCGACGGTGTCGGATTTGGTAGACGCGCTAGCTTCAGGTGTTTGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                                                           <  2:774326/140‑1 (MQ=21)                                                        tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCgg                                                                          >  2:1301753/1‑140 (MQ=255)                                                        tgACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTAAAAAAGCAGTATTTCgg                                                                          >  2:1495207/1‑140 (MQ=255)                                                            aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                                                                      <  2:992434/140‑1 (MQ=255)                                                            aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                                                                      <  2:992222/140‑1 (MQ=255)                                                            aaTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAAATGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                                                                      <  2:992293/140‑1 (MQ=255)                                                              tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                                                                    >  2:1374560/1‑140 (MQ=255)                                                              tGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACGTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                                                                    >  2:1374610/1‑140 (MQ=255)                                                                 gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                                                                 <  1:741672/140‑1 (MQ=255)                                                                 gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                                                                 <  2:496505/140‑1 (MQ=255)                                                                      tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt                                                            >  2:881647/1‑140 (MQ=255)                                                                       ggCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAACAGCAGTATTTCGGCGAGTAGCGCAGCtt                                                           <  2:126961/140‑1 (MQ=255)                                                                        gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTg                                                          >  2:1699701/1‑140 (MQ=255)                                                                        gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTg                                                          >  2:1699609/1‑140 (MQ=255)                                                                         cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTgg                                                         >  1:1715480/1‑140 (MQ=255)                                                                            aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg                                                      <  1:1526741/140‑1 (MQ=255)                                                                            aaTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg                                                      <  1:263537/140‑1 (MQ=255)                                                                               tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcgc                                                   >  2:353528/1‑140 (MQ=255)                                                                               tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTAGGCGAGTAGCGCAGCTTGGTAgcgc                                                   >  2:947133/1‑140 (MQ=255)                                                                                 gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  1:113686/140‑1 (MQ=255)                                                                                 gTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCaa                                                 <  1:1245516/140‑1 (MQ=255)                                                                                   aGACGCGCTAGCTTCAGGTGTTAGGGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACt                                               <  2:398065/140‑1 (MQ=255)                                                                                       gcgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGtt                                           <  2:637789/140‑1 (MQ=255)                                                                                                tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAg                                  <  1:351554/140‑1 (MQ=255)                                                                                                   ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCGCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCCGTAGTTCGGCGAGTCGCGCAGCTGGGTCGCGCCACTGGTTTGGGACCAGTgg                               >  2:326749/1‑140 (MQ=37)                                                                                                       ttAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACTACTTTTAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  2:300206/1‑140 (MQ=255)                                                                                                       ttAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACTACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  2:300219/1‑140 (MQ=255)                                                                                                       ttAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACTACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCg                           >  2:473855/1‑140 (MQ=255)                                                                                                        tAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCgg                          <  2:61515/140‑1 (MQ=255)                                                                                                        tAGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCgg                          <  2:473825/140‑1 (MQ=255)                                                                                                         aGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAc                                     <  2:974114/128‑1 (MQ=255)                                                                                                         aGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAc                                     >  1:974114/1‑128 (MQ=255)                                                                                                         aGTGTCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGa                         >  1:354656/1‑140 (MQ=255)                                                                                                             tCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAACAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     <  2:118187/140‑1 (MQ=255)                                                                                                             tCCTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGtt                     <  2:173809/140‑1 (MQ=255)                                                                                                               cTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCg                   >  1:543311/1‑140 (MQ=255)                                                                                                               cTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCg                   >  1:1537661/1‑140 (MQ=255)                                                                                                               cTTACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCg                   >  1:727907/1‑140 (MQ=255)                                                                                                                 tACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  1:265370/140‑1 (MQ=255)                                                                                                                 tACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 <  1:396649/140‑1 (MQ=255)                                                                                                                 tACGGACGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGaa                 >  1:437364/1‑140 (MQ=255)                                                                                                                       cGTGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACAGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctct           >  1:1574564/1‑140 (MQ=255)                                                                                                                        gTGGGGGATCAAGTC‑CCCCCCCCTCGCACCACGACTATAAAGAATAGAAATAAAAAATCAAAAAGCATTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACAGGTTAGGGACCAGAGTGTCGGAGGTTCGAATCctctc          >  1:880357/1‑140 (MQ=255)                                                                                                                         tGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctctct         >  1:959354/1‑140 (MQ=255)                                                                                                                         tGGGGGTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTAAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctctca         >  1:600051/1‑139 (MQ=255)                                                                                                                         tGGGGGTTCAAGTC‑CCCCCCCCACGCACCACGACTTTAAAGAATTTAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCGTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCctctct         >  1:600052/1‑140 (MQ=39)                                                                                                                           ggggTTCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCg       <  1:1365458/140‑1 (MQ=255)                                                                                                                                tCAAGTC‑CCCCCCCCTCGCACCACGACTTTTAAGAATTGAACTAAAACTTCAAACAGCAGTATTTCGGCGAGTAGCGCAGCGTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:1495379/140‑1 (MQ=38)                                                                                                                                tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAACATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGGTTGGGACCAGTGGGTCGGAGGTTCGACTCCTCTCTCGCCGAc  <  2:426858/140‑1 (MQ=255)                                                                                                                                tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:1768987/140‑1 (MQ=255)                                                                                                                                tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  1:1768785/140‑1 (MQ=255)                                                                                                                                tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:1495373/140‑1 (MQ=255)                                                                                                                                tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  <  2:1503777/140‑1 (MQ=255)                                                                                                                                tCAAGTC‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTAAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAc  >  1:567878/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                       TGATTCCAGTTGTCGTGGGTTCGAATCCCATTAGCCACCCCATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGT‑‑CCCCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGAC  >  NC_000913/3982544‑3982809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑