BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,728,120	T→G	5.3%	T418P (ACG→CCG)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,120	0	T	G	5.3%	1687.3 / 17.8	566	T418P (ACG→CCG)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base T (164/357); new base G (43/2); total (207/359)
Fisher's exact test for biased strand distribution p-value = 3.67e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 580 total aligned reads displayed.

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  NC_000913/3727971‑3728269

  gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGtcc                                                                                                                                                     >  2:5303940/1‑148 (MQ=255)

    tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCCCCGGACgg                                                                                                                                                   >  1:4052143/1‑150 (MQ=255)

    tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACgg                                                                                                                                                   >  1:3012081/1‑150 (MQ=255)

       tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTaa                                                                                                                                                <  2:218631/150‑1 (MQ=255)

       tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑GCCCCCCCGTGCGGtgg                                                                                                                                                >  2:1531483/1‑148 (MQ=255)

        aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAAt                                                                                                                                               <  2:420546/150‑1 (MQ=255)

           cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGTGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGa                                                                                                                                            <  2:1407343/150‑1 (MQ=255)

            ggCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAg                                                                                                                                           <  2:1795620/150‑1 (MQ=255)

             gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGTacgcgtgc                                                                                                                                          >  2:1156589/1‑143 (MQ=255)

              cATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCGCCCCGTGCGGTGATCGGgcg                                                                                                                                         >  1:5494671/1‑149 (MQ=255)

                tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctgc                                                                                                                                       <  1:3261432/150‑1 (MQ=255)

                  aCGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGctgctg                                                                                                                                     >  2:1951976/1‑150 (MQ=255)

                   cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                                                    <  1:4531720/150‑1 (MQ=255)

                       gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                                                <  2:4048157/150‑1 (MQ=255)

                         gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                                                              <  1:5194210/150‑1 (MQ=255)

                            tCTTGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                                                           <  2:5481623/150‑1 (MQ=255)

                               ggTAGATGCGACTGTTCTCACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                        <  2:3589386/150‑1 (MQ=255)

                               ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGTACTCGTGCTGCTGACCGCTGagttcc                                                                                                                        >  2:5688717/1‑145 (MQ=255)

                               ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATcc                                                                                                                        <  1:584096/150‑1 (MQ=255)

                                 tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                                      <  1:1000413/150‑1 (MQ=255)

                                 tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                                      <  1:2869880/150‑1 (MQ=255)

                                 tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                                      <  1:1672732/150‑1 (MQ=255)

                                   gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                    <  2:4584099/150‑1 (MQ=255)

                                   gATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCa                                                                                                                    <  2:3012081/150‑1 (MQ=255)

                                    aTGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                                                   <  1:1321531/150‑1 (MQ=255)

                                     tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGc                                                                                                                  >  2:4643387/1‑150 (MQ=255)

                                      gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGTCAGCGAGCTGCTGACCGCTGAGATCCGCCAGcg                                                                                                                 >  2:4722722/1‑149 (MQ=255)

                                        gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGGTGCGATCCGCCAGCGTc                                                                                                               >  2:783959/1‑150 (MQ=255)

                                        gACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATc                                                                                                               <  1:4380682/150‑1 (MQ=255)

                                        gACGGTTCTAACGGTAGGTGCGGCAACAAGTCACTGAGCGCTGTCTCTGGAGTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATc                                                                                                               <  2:4998957/150‑1 (MQ=255)

                                              tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                                         <  2:2929894/150‑1 (MQ=255)

                                              tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                                         <  1:4942288/150‑1 (MQ=255)

                                              tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                                         <  1:957775/150‑1 (MQ=255)

                                                   cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATGGAGCTGCTGACCGCTGGTATCCGCCAGGATCTGACGCCAGtg                                                                                                    >  2:4767042/1‑149 (MQ=255)

                                                   cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTAc                                                                                                   >  2:4105962/1‑150 (MQ=255)

                                                       aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                                                                <  1:2348997/150‑1 (MQ=255)

                                                        gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTGATCGAGCTGCTGGCCGCTGAGCTCCGCCCGCGTCTGACGCCCGTGCTCAc                                                                                               >  2:871088/1‑150 (MQ=255)

                                                         ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGCGTCGGGCTGCTGGCCGCTGAGATCCGCCGGCCTCTGGCGCCAGTCCTCACt                                                                                              >  2:3822734/1‑150 (MQ=255)

                                                             ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcgc                                                                                          >  2:5198792/1‑150 (MQ=255)

                                                             ggCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGTGCGGTCCGCGGGCTGCTGACCGCGGGTGTCCGCGGGCGTGGGACGGCGGTGCTCGCTAcgg                                                                                          >  1:39360/1‑149 (MQ=255)

                                                                aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGGAATCGAGCTGGTGACCGCTGGTATCCGCCCGCATCTGCCGCCAGTACGCGCTTGGcgcc                                                                                       >  2:3521741/1‑150 (MQ=255)

                                                                  caaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                                     <  1:215670/150‑1 (MQ=255)

                                                                     ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                  <  2:2467171/150‑1 (MQ=255)

                                                                     ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                                  <  2:677076/150‑1 (MQ=255)

                                                                       cAATGAGCGATTTCTCTGGATTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                                <  2:3759855/150‑1 (MQ=255)

                                                                        aaTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGGTATCCGCCAGCAGCGGACGCCAGGACTCACGACGCGCCCCGccccc                                                                               >  1:2742225/1‑150 (MQ=255)

                                                                         aTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                                              <  1:4168366/150‑1 (MQ=255)

                                                                          tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTTCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                             <  2:4442257/150‑1 (MQ=255)

                                                                           gagagaTTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                            <  2:388985/150‑1 (MQ=255)

                                                                             gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTGCGGGGGTCGGGCTGCTGGCCGCTGATATGCGCCCGCGTCGGGCGCCGGTACTCGCTGCGCGCCCCGcccccgctcg                                                                          >  2:237786/1‑145 (MQ=255)

                                                                               gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                                        <  1:3573917/150‑1 (MQ=255)

                                                                                  ttCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACTCGCCCCGCCCCCAATCAACGTa                                                                     <  2:1080514/150‑1 (MQ=255)

                                                                                    ctctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTGGTCGGGCTGCTGGCCGCTGTTGTCCGCCCGCGTCTGTCGCCGGTGCTCACTCCGCGCCCCGCCCCCAATCAACGTacc                                                                   >  2:4350279/1‑148 (MQ=255)

                                                                                       tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCTCACAG‑CCCCCCCCGTGCGGTCAGCGGGCTGCTGGCCGCGGATATCCGCGCGCATCTGTCGCCAGTCCTCACTACGCGCCCCGCCCCCGATCAACGtgccgct                                                                >  1:1496669/1‑144 (MQ=255)

                                                                                       tGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTTCGGTACTCGAGCTGCTGACCGCTGGTATGCGCCGGCAGCTGGCGCCAGGAGTCACTACGCGCCCCGCCCCCCAGCACCGGAACCCt                                                                >  2:4943330/1‑150 (MQ=255)

                                                                                          aTTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                                             <  1:5643761/150‑1 (MQ=255)

                                                                                          aTTCGCCGCGATCAGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCTCCCCGCCCCCAATCAACGTAACACTTTg                                                             <  2:2022435/150‑1 (MQ=255)

                                                                                           ttCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGc                                                            <  2:5255290/150‑1 (MQ=255)

                                                                                            tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                           <  1:375320/150‑1 (MQ=255)

                                                                                            tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                           <  1:4714623/150‑1 (MQ=255)

                                                                                             cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                                          <  1:3350277/150‑1 (MQ=255)

                                                                                               ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                                        <  2:369553/150‑1 (MQ=255)

                                                                                                 gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGAATCGAGCTGCTGGCCGCTGGTGTCCGCCCGCGTCTGACGCCGGTACTCGCTTCGCGCCCCGCCCCCGAGCAACGTAACACGGTGCggggtg                                                      >  2:2692818/1‑146 (MQ=255)

                                                                                                 gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGGCGGGACTCGCGGGGCTGGCCGCGGGGATCCGCCCGCAGCTGTCGCCCGTTCGCCCGGCGCGCCCCGCCCCCCCGCCACGGAACACGTTGCgggggg                                                      >  2:5048119/1‑146 (MQ=255)

                                                                                                    aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGTAATCGCGCTGCTGACCGCGGGTATCCGCCAGCATCTGGCGCCAGTACTCACTACGCGCCCCGCCCCCCATCCACGTCACCCTTTGCGGTTTACTa                                                   >  2:543119/1‑150 (MQ=255)

                                                                                                       tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGGACGGTAATCGGGCTGCTGGCCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTACCACGTTGCGGGTGAATCCCg                                                >  2:1286836/1‑150 (MQ=255)

                                                                                                       tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTCATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGTCGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACGCTTTGCGGTGTAATAGCg                                                >  2:2619185/1‑150 (MQ=255)

                                                                                                        gcgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGc                                               <  1:916831/150‑1 (MQ=255)

                                                                                                         cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCa                                              <  2:483119/150‑1 (MQ=255)

                                                                                                          gcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAg                                             <  1:797475/150‑1 (MQ=255)

                                                                                                                cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                                       <  1:5152856/150‑1 (MQ=255)

                                                                                                                  tgctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                     <  2:4267389/150‑1 (MQ=255)

                                                                                                                  tgctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                                     <  1:5565670/150‑1 (MQ=255)

                                                                                                                     tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTTATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCTATCAACGTAACACTTTGCGGTTTAATACCGCAGGCTTGCACGAc                                  <  2:3131911/150‑1 (MQ=255)

                                                                                                                     tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGAc                                  <  1:4612671/150‑1 (MQ=255)

                                                                                                                       cgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACAt                                <  1:1986803/150‑1 (MQ=255)

                                                                                                                         cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGTCATcc                              <  1:3502173/150‑1 (MQ=255)

                                                                                                                         cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                              >  2:5502090/1‑150 (MQ=255)

                                                                                                                         cccAGTGCTGGCCCCACAT‑CCCCCCCCGGACGGTAATCGAGCTGCTGGCCGCTGATATCCGCCAGCGTCGGGCGCCAGTACGCCCGACGCGCCCCGCCCCCCATCAACGTAACACGTTGCGGTTTAATACCGCAGGCAGGCACGGCCTcc                              >  1:4771269/1‑150 (MQ=255)

                                                                                                                          ccAGGGCTGGCCCCACCTCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                              <  1:569094/150‑1 (MQ=255)

                                                                                                                           cAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGAAGGCATTCAAGACATCCAt                            >  2:60081/1‑150 (MQ=255)

                                                                                                                                 tGGCCCCACAT‑CCCCCCCCGTTCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc                      >  2:534702/1‑150 (MQ=255)

                                                                                                                                 tGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTGAATACCGCAGGCATGCACGACATCCATGCCATc                      >  2:5602367/1‑150 (MQ=255)

                                                                                                                                  ggCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                     <  1:1123084/150‑1 (MQ=255)

                                                                                                                                  ggCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCt                     <  1:5254297/150‑1 (MQ=255)

                                                                                                                                   gCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGGTTAACGCAGATATCCGCCAGCATGTGACGCCAGTAATCACTACGCGCCCCGCCCCCAAAAAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCCCGACATCAATGCCATCTg                    >  1:2467522/1‑150 (MQ=255)

                                                                                                                                    ccccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGc                   <  1:4862103/150‑1 (MQ=255)

                                                                                                                                     cccAAAT‑ACCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCAATCATATGACGACAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACTCTTTGCGGTTTAATTCCTCAGGCATGCACGACATCCATTCCATCTGcc                  >  2:2404881/1‑150 (MQ=255)

                                                                                                                                      ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                 <  1:5396888/150‑1 (MQ=255)

                                                                                                                                      ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa                 <  1:775557/150‑1 (MQ=255)

                                                                                                                                      ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTGTAATACCGCAGGCATGCACGACATCCATGCCCTCTGCCa                 >  2:2797232/1‑150 (MQ=255)

                                                                                                                                         caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACCCTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg              <  2:2074287/150‑1 (MQ=255)

                                                                                                                                         caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg              <  2:4893116/150‑1 (MQ=255)

                                                                                                                                           t‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGGACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCCTGCACGACCTCCATGCCATCTGCCAGCGCa            >  1:5092502/1‑150 (MQ=255)

                                                                                                                                           t‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCa            <  1:1254101/150‑1 (MQ=255)

                                                                                                                                             ccccccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCAt           <  2:1620460/150‑1 (MQ=255)

                                                                                                                                               ccccccGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATaa         <  2:2310061/150‑1 (MQ=255)

                                                                                                                                                cccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAc        >  2:3271841/1‑150 (MQ=255)

                                                                                                                                                 ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATTCCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc       <  1:4730942/150‑1 (MQ=255)

                                                                                                                                                 ccccGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAAcc       <  1:2700061/150‑1 (MQ=255)

TGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGCATAACCCACGC  >  NC_000913/3727971‑3728269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`