| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 291 | A→T | 5.8% | intergenic (+36/‑46) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 291 | 0 | A | T | 5.8% | 1132.9 / 42.5 | 324 | intergenic (+36/‑46) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (132/171); new base T (10/9); total (143/181) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
Only 100 of 327 total aligned reads displayed.
ATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCAC > NC_000913/142‑440 | ataGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGa > 1:5273921/1‑150 (MQ=255) taGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGAc < 2:711492/150‑1 (MQ=255) aGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACa > 1:3418809/1‑150 (MQ=255) gCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑Gt > 2:1762443/1‑150 (MQ=255) aGCGCACAGACATATAAAAATTACAGACTACACAACACCCATGAAACGCATTAGCACCCCCATTACCACCACCATCACCATTCCCCCCGGTAACGGTGCGGGCTGACGCTTACAGTAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg < 1:1181882/150‑1 (MQ=255) aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCTTTAGCACCACCATTACCACCACCATCACCATTACCACCGTTATGGGTGGGGGCTGACGCGTACAGTAAACACATAAAAAAGCCCGCACCTGACA‑GTGCgg < 1:1758814/150‑1 (MQ=255) aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg < 2:4542820/150‑1 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGTAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCggg < 1:3179241/150‑1 (MQ=255) cgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGc > 1:6413255/1‑150 (MQ=255) cgcACACACAGATAAAAATTACAGAGAACACAACAGCCATGAAACGCATTCGCACCACCATGACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACTGGAAACACACAAAAAAGCCCGCACCTGACA‑GTGCGGGc < 1:1283126/150‑1 (MQ=255) agacagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑ttttt > 2:5852835/1‑150 (MQ=255) cagaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAATAGCCCGCACCTGACA‑GTGCGGGC‑‑tttttttt > 1:2563341/1‑150 (MQ=255) agaTAAAAATTACAGAGTACACCACATCCCTGACCCGCATTAGCACCACCATTACCACCACCATCACCCTTCCCACAGGTAACGGTGCGGGGTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑ttttttttt < 2:1504142/150‑1 (MQ=255) aTTCCAGAGTACACAACTTCCAAGAAACGCTTTCGCCCCACCATTACCACCACCCTCACCATTCCCCCAGGTAACTGTGCGGGCGGACGCTTACAGGAAACAAAGAAAAACGCCGCAACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCaaa < 2:125383/150‑1 (MQ=255) aTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACTGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCaaa < 2:2552130/150‑1 (MQ=255) ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCT‑TTTTTTTTTCGACCaaa < 2:2898690/150‑1 (MQ=255) ttACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAg > 1:1413030/1‑150 (MQ=255) cAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTa > 2:6185219/1‑150 (MQ=255) agagTACACAAAATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTaa < 1:2135066/150‑1 (MQ=255) tACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAg > 1:6003531/1‑150 (MQ=255) tACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAg > 2:4504943/1‑150 (MQ=255) cacaACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGt < 1:2429644/150‑1 (MQ=255) acaacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTa < 1:52162/150‑1 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAGACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac > 2:4234910/1‑150 (MQ=255) aacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTaac > 2:3877404/1‑150 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGGCA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTaacaa > 1:2840364/1‑150 (MQ=255) caTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTaacaa < 1:5482246/150‑1 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCT‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAt > 1:4041170/1‑150 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg > 1:6364923/1‑150 (MQ=255) tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc > 1:2114119/1‑150 (MQ=255) gAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCT‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc < 2:2105085/150‑1 (MQ=255) aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACC‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACACCCATGCGa > 2:2383231/1‑150 (MQ=255) aCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgt > 1:4532660/1‑150 (MQ=255) gCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAATGGTAACGAGGTAACAACCATGCGAgtgt > 2:2020431/1‑150 (MQ=255) gCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtgt > 2:2603118/1‑150 (MQ=255) tAGCACCCCCATTACCACCACCATCACCATTACCCCCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:1961299/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:386959/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:3719797/150‑1 (MQ=255) caccacCATTACCACCACCATCACGATTAACTCATGTAACGGTGCGGGCTGACGCGTACTGGAAACACAGAAAAAAGACCGCACCTGACA‑TTGCGTGA‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACACCCATGCGAGTGTTGAAGtt < 2:5614671/150‑1 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg > 1:1515101/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTCACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTACCAACCATGCGCGGGTTGAAGTTcgg > 2:4455047/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg > 1:4102956/1‑150 (MQ=255) ccaTTACCACCACCATCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggcg < 1:6123191/150‑1 (MQ=255) atgaccacccccATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGt < 2:5447887/147‑1 (MQ=255) ttACCACCACCATCCCCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa < 1:4894640/150‑1 (MQ=255) ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa < 2:2318351/150‑1 (MQ=255) ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa > 2:4791794/1‑150 (MQ=255) ttACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTa > 1:3538904/1‑150 (MQ=255) caccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATc < 1:3240635/150‑1 (MQ=255) accaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAAAACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTAGACCAAAGGTAAAGAGGTAAAAACCATGCGAGTGTTGAAGTTCGGCGGTACATCa > 2:4993726/1‑150 (MQ=255) caccaacaccatTACCACCGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCCGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGt < 2:2472249/150‑1 (MQ=255) atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCa > 1:6571546/1‑150 (MQ=255) tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCaa > 2:4282626/1‑150 (MQ=255) accatTACCACAGGTACCGGGGCGGGCTGACGCGTACAGGACACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTCACGAGGTAACACCCCGGCGAGTCTTGAAGTTCGGCGCGACAGCACTGGcccag > 2:5045441/1‑146 (MQ=255) catTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc < 1:1851175/150‑1 (MQ=255) cagtACCACAGGTAACGGTGCGGGCTGACGCGCACAGGAAACACACAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGc < 2:5708808/147‑1 (MQ=255) agtACCACAGGTAACGGTGCGGGCTGACGAGTACAGGAAACCCCCCAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCa < 2:6281226/148‑1 (MQ=255) ttACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAg > 2:3918877/1‑150 (MQ=255) aCCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa < 1:1632451/150‑1 (MQ=255) ccACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCT‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGAGGCAAATGCAGaa > 1:3053830/1‑150 (MQ=255) cacaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGCTTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGaa < 1:3756086/150‑1 (MQ=255) cacaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACg > 1:509909/1‑150 (MQ=255) ggTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtttt > 1:4329141/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc > 1:758274/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc > 1:1006051/1‑150 (MQ=255) tAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCt < 1:3978324/150‑1 (MQ=255) aCGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGc < 2:5199756/150‑1 (MQ=255) ggTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt < 1:5018015/150‑1 (MQ=255) tGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgtgt > 1:2363319/1‑150 (MQ=255) gCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGtt < 1:365931/150‑1 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt > 1:1469457/1‑99 (MQ=255) ggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGcc > 2:3162759/1‑150 (MQ=255) cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGa < 2:1413030/150‑1 (MQ=255) cTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGa < 1:623465/150‑1 (MQ=255) gACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGata > 2:2675875/1‑150 (MQ=255) aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat > 2:798810/1‑150 (MQ=255) aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat > 2:5671714/1‑150 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:4281659/150‑1 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg < 2:365280/150‑1 (MQ=255) aaGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaa < 2:408665/149‑1 (MQ=255) ggAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAACGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCTGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg < 1:2792416/150‑1 (MQ=255) ggAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTACCAACCATGCGCGTGTTGAAGTTCGGCGGTCCATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGACAg > 2:5999107/1‑150 (MQ=255) ggAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAg < 1:958326/150‑1 (MQ=255) aaCACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCaa < 1:2603118/150‑1 (MQ=255) aaCACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCaa < 1:570250/150‑1 (MQ=255) gaaaaaaaaaGCCCGCA‑CGGTCAGGTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGGTGAAGTTCGGCCGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:4640070/5‑150 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc < 1:4791794/150‑1 (MQ=255) aGAAAAAAGCCCGCACCTGACA‑GTGCGGTC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca < 2:3406159/150‑1 (MQ=255) aGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCca < 1:4141586/150‑1 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg < 2:3051667/150‑1 (MQ=255) cccGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggc > 2:2223312/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTTAAGTTCGGCGGTACAACAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca > 1:5996202/1‑150 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcc > 2:2782047/1‑149 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcc > 1:2682923/1‑149 (MQ=255) ccGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACCGTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca > 1:6441777/1‑150 (MQ=255) gCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcagg < 1:2979342/150‑1 (MQ=255) aCCTGACA‑GTGCGGGC‑‑TTTTTTTTTAGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTATGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTg > 1:672907/1‑150 (MQ=255) ccTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTgg < 1:5852624/150‑1 (MQ=255) cTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGc < 2:6571546/150‑1 (MQ=255) gACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCa < 1:891521/150‑1 (MQ=255) | ATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGGC‑‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCAC > NC_000913/142‑440 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |