| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 781,670 | A→T | 6.0% | intergenic (+18/‑415) | lysQ → / → nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 781,670 | 0 | A | T | 6.0% | 603.3 / 29.5 | 233 | intergenic (+18/‑415) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
| Reads supporting (aligned to +/- strand): ref base A (117/96); new base T (4/10); total (126/107) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.64e-01 | |||||||||||
Only 100 of 236 total aligned reads displayed.
GAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA > NC_000913/781521‑781817 | gAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTa > 1:5470101/1‑150 (MQ=255) taaTCCTGCACGACCCAACATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAg < 2:5072270/148‑1 (MQ=255) tCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAg > 1:1319748/1‑150 (MQ=255) tGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTCAAGGCGCtt > 2:3389496/1‑150 (MQ=255) tGCAAGACCCACCATCCTTATTGATTTAGGCAGCATAAGCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCtt < 2:4496273/150‑1 (MQ=17) gCACGACCCACCATCCTGAATTATTAAGGCAGCATAATCCCGCATGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGTTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCttt < 1:1197788/150‑1 (MQ=255) gCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCttt > 1:4495136/1‑150 (MQ=255) cccGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCTTTAGGGCGCtttt < 1:3117922/148‑1 (MQ=255) cACGACCGACCATCCTTTTTTATTTATGCAGCCTAATCCCGGTAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCtttt < 2:5344934/150‑1 (MQ=14) cACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCTTTAGGGCGCtttt < 2:1936042/150‑1 (MQ=255) aCGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCttttt > 1:4341404/1‑150 (MQ=255) cGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCTTTAGGGCGCtttttt < 2:1786100/150‑1 (MQ=255) cccACCATCCTGAATGATTAAGGCAGCATACCCCCGCACGGGGTCGTTATCTCAGTGGTTAGAGCAGTTCACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAA‑GCGCCTTTAGGGCGCTTTTTTTCt < 1:1147844/150‑1 (MQ=17) cacaccaTCCTGTTTGATTAATGCATCTTAATCCCGGAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCt < 2:2062591/148‑1 (MQ=17) ccaccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGCAAAAAA‑GCGCCTTTAGGGCGCTTTTTTacat > 2:5242796‑M2/1‑146 (MQ=255) caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTAt > 1:4633906/1‑150 (MQ=255) caccaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTAt > 2:4069015/1‑150 (MQ=255) accaTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTacattg > 2:2842051‑M2/1‑144 (MQ=255) ccaTCCTGAATGATTCAGGCAGCATACTCCCGCACGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCt < 2:3285419/150‑1 (MQ=255) cgcccgAAAGATTAAGGCAGCATACTCCCGCAAGGGGTCGTTCGCTCAGTTGGTAGAGCAGTTGACTTTTAATCACTTGGTCGCAGGTTCGAATCCTGCACGACCCCCCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGc < 1:418786/145‑1 (MQ=17) aTCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGc < 1:1266848/150‑1 (MQ=255) ccggcaagATTTAGGCCGCCTACGCCCGCTAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGa < 2:3804495/143‑1 (MQ=11) cTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATAAGCGAt > 2:4834408/1‑150 (MQ=255) tGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATa > 2:2633753/1‑150 (MQ=255) gAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCGGCGATAc > 2:4570804/1‑150 (MQ=255) aTGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCGAAAGGCGCTTTTTTGCTATCTGCGATACTc < 2:3087409/150‑1 (MQ=255) tGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCa < 2:2265849/150‑1 (MQ=255) aTTAAGGCAGCATAATCCCGCTAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa < 2:4426116/150‑1 (MQ=255) aTTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa < 2:782611/150‑1 (MQ=255) gcagcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCg > 2:1520730/1‑150 (MQ=255) agcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGaa > 2:1341104/1‑150 (MQ=255) caTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATACCAAcc > 2:4113771/1‑150 (MQ=255) aTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCtttttt < 2:3465197/120‑1 (MQ=255) tAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTg < 1:1198174/150‑1 (MQ=255) tCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTgcag > 2:3960605/1‑150 (MQ=39) aGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt < 1:4678950/150‑1 (MQ=33) aGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt < 1:662897/150‑1 (MQ=33) aGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt < 2:4018495/150‑1 (MQ=33) gggTCGTTTGTCAAGTTGGTAGAGCTGTTGAATTTTAAACAATTGGCCGACGGTTCGAAGCCTGCACGACCCACCAATTTAAAAAA‑GCGCCCAAACGCCGATTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga < 2:4632020/150‑1 (MQ=11) gggTCGGTAGCTGAGTTTGTTGTTCTGTTGTCTTTTAATCTATTGGTCGCATGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttga < 1:1413928/150‑1 (MQ=11) gTCGTTAGCTCCGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagt < 1:5209880/150‑1 (MQ=17) gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga > 1:2000925/1‑150 (MQ=32) ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAc < 1:5292019/129‑1 (MQ=32) ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAc > 2:5292019/1‑129 (MQ=32) ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttgag > 1:4995909/1‑150 (MQ=32) tAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAgc < 1:4289101/150‑1 (MQ=32) gCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAgcgc < 2:1319748/150‑1 (MQ=32) cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCa < 1:818955/150‑1 (MQ=32) cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCa > 1:5063542/1‑150 (MQ=32) tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg < 1:2633628/150‑1 (MQ=32) gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTCAAGGCGCTTTTTTGCTATCTGCGATACTCAAGGATTCGAACCTGCAGCAGGTGTGTGTTGAGCGCAGCGa > 2:599863/1‑150 (MQ=255) gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGa > 2:3279174/1‑150 (MQ=17) tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaaa < 1:4743045/150‑1 (MQ=32) gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCCGCAGGTTTGAGTTGAGCGCAGCGAaaca > 1:3438665/1‑150 (MQ=17) tAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaacaa < 2:4542379/150‑1 (MQ=32) gaaGTTTAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACTACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCTAAACAACGGa < 2:1869540/148‑1 (MQ=255) aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGc > 1:5101210/1‑150 (MQ=32) gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGcc > 1:3053346/1‑150 (MQ=32) ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCAGAAGCAGTTAGGAGTTGAGCGCAGCGAAACAACGGAGCCg > 1:2219301/1‑150 (MQ=11) tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTAAAAGATTCCAACCTGCAGCAGGTTTGAGAGGAGCGCAGAGAAACAAAGGAGCCGc > 1:1696659/1‑150 (MQ=11) ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc > 2:1988189/1‑150 (MQ=32) ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACGCAAAGATACGATCCTGCACCAGTTTTCAGATGATCGCAGCGAAGCGCACGAGCCGCTcgc > 1:3612563/1‑150 (MQ=11) aTCATTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGATTTTTTGCTATCTGCGATACTCAAATATTCGAACCTGCAGAAGTTTTGAGTTGAGCGCAGCTAAACAACGGAGCCGCTCGCGGCGa < 2:3233045/150‑1 (MQ=11) aTCAATTGGCCGCAGGTTCGAACCCTGCCCGACCCACCACTGTAAAAAC‑GCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGGTTTGTGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGa < 2:2360506/150‑1 (MQ=11) tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGATCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc > 2:2296445/1‑150 (MQ=17) tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAACTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc > 1:404248/1‑150 (MQ=17) aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACgg > 2:1472973/1‑150 (MQ=32) ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCACAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGcc > 2:381594/1‑150 (MQ=17) ttGGGCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGcc < 2:4120873/150‑1 (MQ=17) tCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTTAGCGCAGCGAAAAAACGGAGCCGCTCGCGGCGACGGCCCGaa < 2:1110520/150‑1 (MQ=12) tCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGaa > 2:419337/1‑150 (MQ=32) tCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGGTGAGCGCAGCGCAACAACGGAGCCGCTCGCGGCGACGGCCCGaa > 2:4223706/1‑150 (MQ=17) cGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATGTGCGATACTCAAATATTCGTACCTGCACCAGGTGTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAg > 1:4378854/1‑150 (MQ=11) gTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcga > 2:2923622/1‑150 (MQ=32) ttCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgag > 1:3682750/1‑150 (MQ=32) gAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgagcga > 1:3017862/1‑150 (MQ=32) aTCCTGCACGACCCACCAATTTAAAAAT‑GCGCCCTAAAGGCGATTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaag < 2:754114/150‑1 (MQ=255) cTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagcga < 2:3032598/150‑1 (MQ=32) cTGCACGACCAACCAATGTAAAAAA‑GCGCCCTAATGGCGCTTTTTTGATATATGCGATACTAAAAGATTCTAACCTGCAGCTGGTTTTTGTTGAGCGCAGCTAAACAACGGATCCGATCGCGGCGACGGCCCGAAGGGCGagcgaagcga < 2:4137630/150‑1 (MQ=11) tGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAg > 2:5110497/1‑150 (MQ=32) aCGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTacattggtgggtcgtgcaggattcgaacctgcgaccaagtgagtacaagtcaactgctctaccaactgggctaacgacccgacgtggtgggtgatgacgggatcgaac < 2:4857347‑M2/150‑109 (MQ=255) aCGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCa < 2:4287498/150‑1 (MQ=32) aCGACCCA‑CAATTTAAAAAACGCCCCCTACGGGGGTTTTTTTGCTTTATGCTATACTCAAAGATTCCACCCTGCTGCTGTTTTGTGTTGCGGGCTCCCAAAAAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCa < 1:4954983/150‑1 (MQ=2) cgccccACCACTGTAAAAAA‑CCGCCCTAAAGGCGCTTTTTTGCTATCTGCTATACTCAAAGATTCGAACCTGCAGCTGTTTTTAGTTGTGCGCAGCGAAACAACGGAGCCGCTCGCTGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCAt < 2:1022029/147‑1 (MQ=11) gACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATTCTGAAAGATTAGAACCTGCAGCAGGTTTGAGTTGAGCGGAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCGGAAGGGCGAGCGAAGCGAGTCATc > 1:1843078/1‑150 (MQ=11) aCCCACCACTGAAAACAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCCACGATTCGAACCTGCAGCTGGTTTGAGTTGAGCGCAGCGACACAACGGAGCCGCTCGCGGCGACGGCCCGCAGGGCGAGCGAAGCGAGTCATcc < 2:4445137/150‑1 (MQ=11) accaATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAATCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa > 1:2005787/1‑150 (MQ=17) accaATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCCGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCa > 1:5650/1‑150 (MQ=17) caATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACg > 2:2094423/1‑150 (MQ=32) caATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACg > 2:2535318/1‑150 (MQ=32) atagcaaaaaa‑GCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGa > 1:2693030/6‑150 (MQ=255) aaTGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGAAGCAGTTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTTCACGa > 2:3527676/1‑150 (MQ=12) tAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACcca > 2:2871430/1‑150 (MQ=32) aaaaaa‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCAACCTGCACGACccac > 1:4071702/1‑150 (MQ=17) aaaaa‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc > 1:155835/1‑150 (MQ=32) aaaaa‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc > 2:665264/1‑150 (MQ=32) aaaa‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGGTTTGTGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca < 2:4992355/150‑1 (MQ=17) aaaa‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca < 2:1634060/150‑1 (MQ=32) aaa‑GCGCCCTAATGGCTATTTTTTGCTATCTGCGATACTCAAATATTCTAACCTGATGCTGGTTTGTGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa < 1:4116458/150‑1 (MQ=11) atgcgcCCTTAACTCGATTTTTTGCTTTCTGCGATCCTCAACGACACGAAACTGCAGCTGGTTTGAGTTGTTGAGAGCGTAACTACGTAGGCTCTCGCTGCTACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAt < 2:5456368/148‑1 (MQ=2) | GAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAA‑GCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA > NC_000913/781521‑781817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |