BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,287,143	G→T	5.5%	noncoding (94/171 nt) P12T (CCA→ACA)	rttR ← tpr ←	rtT sRNA, processed from tyrT transcript protamine‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,143	0	G	T	5.5%	722.6 / 4.2	253	noncoding (94/171 nt) P12T (CCA→ACA)	rttR tpr	rtT sRNA, processed from tyrT transcript protamine‑like protein
Reads supporting (aligned to +/- strand): ref base G (136/92); new base T (10/10); total (150/103)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

CATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTT  >  NC_000913/1286996‑1287292

 aTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGgcgc                                                                                                                                                     >  2:1732415/1‑150 (MQ=255)

 aTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCGGCGGCAACGCGCACTCGCTGgcgc                                                                                                                                                     >  1:2386492/1‑150 (MQ=255)

  tCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCt                                                                                                                                                    >  2:6079605/1‑150 (MQ=255)

  tCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCt                                                                                                                                                    <  1:7756986/150‑1 (MQ=255)

     ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCACTCTCGCTGTCGCTCGa                                                                                                                                                 >  2:5739797/1‑150 (MQ=255)

      tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCACCCGCGCACGCGGGcgcgcgcg                                                                                                                                                >  1:803652/1‑148 (MQ=14)

       ccccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCCGCAACCCACAATCGCGGGCGCACCAGt                                                                                                                                               >  1:3908178/1‑150 (MQ=14)

        cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTc                                                                                                                                              >  2:4516071/1‑150 (MQ=255)

          cGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGa                                                                                                                                            <  1:1327226/150‑1 (MQ=255)

           gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                           <  1:3454332/150‑1 (MQ=255)

                 gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCTCTTCTCCCTTCGGGTCGTTGCCTGCGGCAACGATCTCTCGCTGTCGCTCAACGCGAACCATgc                                                                                                                                     >  2:3606602/1‑149 (MQ=14)

                 gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTAGTTGCCTGCGGCAACGCTATCTCGCTGGAGCTCGAGTCGAAACATgg                                                                                                                                     >  2:1420487/1‑150 (MQ=14)

                 gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCCCATCGCCCTTCGGGTCGTTGCCGGCCCCAAAGCTCTCTCGCTCGCCCGCCACTCGAACCTTgg                                                                                                                                     >  2:6362844/1‑150 (MQ=14)

                  cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                    <  1:2260113/150‑1 (MQ=255)

                    gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                  >  2:5700578/1‑150 (MQ=255)

                    gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCTTCGGTTCGATTCCTCACGCAAAGCACTCTCGCTGGCCCTAGAGTCGAACCTTGGTCg                                                                                                                                  >  2:1038486/1‑150 (MQ=14)

                         tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGTCTCGAACCGTGGTAGAAGCt                                                                                                                             >  2:5492153/1‑150 (MQ=14)

                         tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGACCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCt                                                                                                                             >  2:3063353/1‑150 (MQ=255)

                             aTTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAAAGCACACACGCTGGCCCTCGAGTCGAAACGTGGTAGAAGCttata                                                                                                                         >  1:4458697/1‑147 (MQ=14)

                              ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAACCCACACAAGCAGGCGCGCGACTCGAAACATTGTCGAAGCATcaca                                                                                                                        >  1:7906020/1‑150 (MQ=14)

                               tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTACGGGTCGTTGCCTGAGGCAAAGCTCTCTCGCTGGCCCACGAGTCGAACCTTGGTCGAAGCTTcacaa                                                                                                                       >  2:2230615/1‑149 (MQ=14)

                                 cgtaTTCGATTGAGATTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATcc                                                                                                                     <  2:2155834/147‑1 (MQ=255)

                                 cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCATcc                                                                                                                     >  2:5071224/1‑150 (MQ=255)

                                 cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTTGCGCTCGAGTCGGAACGTGGTCGAAGAGTCTCATcc                                                                                                                     >  2:8291236/1‑150 (MQ=14)

                                   gATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCtt                                                                                                                   >  2:5296866/1‑150 (MQ=255)

                                     ttCGCTTGAGAGTTCAGGGACTTTCGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCATTGTCGAAGCTTCTCAAccaaac                                                                                                                 >  1:7603849/1‑146 (MQ=14)

                                     ttCGCTTGAGAGTTCAGGGACTGTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCCGCGCTGCGCGCATCGCCCTTCGGGACGTTGCCTGAGGCAACGCTCTCTCGCTTTCGCTCGCCGCGAACCGTCGTCGAAGGTTCTCACCCTTcc                                                                                                                 >  2:2944905/1‑150 (MQ=14)

                                        gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCGCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATACGTccccc                                                                                                              >  1:8180784/1‑149 (MQ=14)

                                          ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCACACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCAACCATCCCCCCa                                                                                                            >  1:1891515/1‑150 (MQ=14)

                                          ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACGAGTCGAAACTTTGTCGAAGCTTCTCAACCATCCCCGCa                                                                                                            >  2:1864535/1‑150 (MQ=14)

                                           tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑t                                                                                                          <  1:9195540/150‑1 (MQ=255)

                                            gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGATCTCTCGCTTTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTACCCgaagg                                                                                                          >  2:4965258/1‑146 (MQ=14)

                                            gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCCCATCGCCCTTCGGGTCGTTGCCGCCGGCAACGCACGCTCGCAGGCCCGCCAGTCGAACCCGGGGCGAAGCATCACAACCATCCCCCCaag                                                                                                          >  2:8056975/1‑148 (MQ=14)

                                                gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCa                                                                                                     <  2:9217429/150‑1 (MQ=255)

                                                gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGACTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGAA‑GGGGcc                                                                                                     >  2:6932955/1‑149 (MQ=255)

                                                 ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGACGTTGCCTGCGGCAACCCTCTCACGCGGGCCCACCAGGAGAACCCTGGTCGAAGCAGCGCAACCTTCCCCGCC‑TGGGccg                                                                                                    >  2:1893735/1‑148 (MQ=14)

                                                  tCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCGTATCAAACTTACCCCCC‑AGGGCAGa                                                                                                   >  1:8937034/1‑150 (MQ=14)

                                                   cAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACACACGCAGGCGCGCGAGTCGAACCCTGGTCGAAGCGTCTCAAACATACCCCCA‑AGGGCCGaa                                                                                                  >  1:3114406/1‑150 (MQ=14)

                                                    aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCGTCGGGTCGTTGCGTGAGGCAAAGCTCTAAAGGTGGCGCGCGACTCGAGCCGTGGTCGAAGCTTCTCATCCTTCCGCGCC‑Tggggcgggg                                                                                                 >  2:7867689/1‑144 (MQ=14)

                                                          ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCATCGCTGCGCTCTTCGCCCTTCGGGACGTGGACTTCGACAACGCGATCTCGCTGCCGCTAGAGTAGAACCTTGGTCGACGCTTCACAAACTTCCCCGCA‑TGGGCAGAATAGTTaa                                                                                           >  2:6843759/1‑150 (MQ=11)

                                                          ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTCATCCTTCCCCCCA‑AGTGCAGAATATTTaa                                                                                           >  2:5845891/1‑150 (MQ=11)

                                                           tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAATCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCCCATTGGGCaaaaattaaaa                                                                                           >  2:195212/1‑143 (MQ=11)

                                                           tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAAt                                                                                          <  1:6825391/150‑1 (MQ=39)

                                                               aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACACACGCGGGCCCTCGACTCGAACCAAGGTCGAAGCATCAAAAACATACCCCCA‑AGGGCAGAAAAATTAAtagag                                                                                      >  1:4784991/1‑146 (MQ=2)

                                                                     aTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCCGCCACCCACACACGCAGGCCCACCAGTCGAACCATGGTCGAAACTTCACAACCATCCCCCCC‑GGGGCCGAAAAATTAATTGCGGAGTCg                                                                                >  1:7561592/1‑150 (MQ=2)

                                                                      tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGt                                                                               <  1:5726925/150‑1 (MQ=37)

                                                                         tggtgtGGGATTGATTTCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                            <  1:7034629/150‑1 (MQ=255)

                                                                           gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCAGCGGCAACGCTCGCTCGCAGGCGCTCGATCCGAACCATGGTCGAAGCATCTCATCCTTACCCCCC‑TGGGCAGAATAATTGATTTCGGATTCGGTgggg                                                                          >  2:4236254/1‑149 (MQ=11)

                                                                               gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGtt                                                                      <  1:3531273/150‑1 (MQ=255)

                                                                                           tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                          >  2:677321/1‑150 (MQ=255)

                                                                                           tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                          <  1:2701595/150‑1 (MQ=255)

                                                                                            cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                                         <  1:6079605/150‑1 (MQ=255)

                                                                                             aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑GGGGCCGAACATTGAAGTGCGGATTCGGGGGGGAGGTCCGGGACGTTTGaa                                                        >  2:9115873/1‑150 (MQ=14)

                                                                                              gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCACTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCCCA‑GGGGCAGAATATTTTATTGCGGGTTCGTTGGGCAGTTCAGGGACGTTTGtaa                                                       >  2:1171574/1‑150 (MQ=14)

                                                                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                      >  2:1518928/1‑150 (MQ=255)

                                                                                               cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                      >  2:6600878/1‑150 (MQ=255)

                                                                                                         ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggt                                            <  1:5921249/150‑1 (MQ=255)

                                                                                                         ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggt                                            >  2:4412200/1‑150 (MQ=255)

                                                                                                               cTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATTGTCGAAGCATCACAACCATCCCCCCA‑TGGGCAGAAAATTTAAAATCGGATTCGTTGGGAAGTTAAGGGACATTTGAAAGTGTTGGTGGTgggggt                                      >  2:3986784/1‑149 (MQ=14)

                                                                                                                ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                     <  1:5601037/150‑1 (MQ=255)

                                                                                                                ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                     <  1:4521737/150‑1 (MQ=255)

                                                                                                                   agagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                   <  2:2618153/129‑1 (MQ=255)

                                                                                                                     gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGTTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                <  2:3415071/150‑1 (MQ=255)

                                                                                                                     gTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGACGCGAACCTTGGGCGAAGGATCTCCACCTTCCCCGCA‑TGGGCAGAAAAGTTAATAGGGGAAGCGTTGGGAAGGGACGGGAGTGTTGAAAGTGATGGTGGGGGGGGactgatg                                >  2:7366667/1‑144 (MQ=9)

                                                                                                                      tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                               <  1:1420472/150‑1 (MQ=255)

                                                                                                                      ccgccgCCCGCGCCAACGCCCTCTCGCTGTCGCTCGAGTCGCACCTCCGTCGAATCTTCTCAGCCTTCCCCTCT‑TGGGCTGAACTACTAACTTCCGATCCGTTGGTCACTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                               <  2:1266001/145‑1 (MQ=9)

                                                                                                                         ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt                                                                                                                                               <  1:1548851/36‑1 (MQ=40)

                                                                                                                         ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                            <  1:1139082/150‑1 (MQ=255)

                                                                                                                          tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtg                                              <  1:4958323/131‑1 (MQ=255)

                                                                                                                          tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                           <  1:5780686/150‑1 (MQ=255)

                                                                                                                          tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCT‑TGGGCAGAATATTTGATTGCGGATTCGTTTGGAAGTTCAGGGACTTTTGAAAGTGAtggtg                                              <  1:3165554/131‑1 (MQ=255)

                                                                                                                             cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                        <  2:803652/150‑1 (MQ=255)

                                                                                                                              tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTGGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                       >  2:1684540/1‑150 (MQ=255)

                                                                                                                              tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCATCTAAAACATACCCCCA‑AGGGCAGAATATTTAAATTCGGGTTAGTTGGGAAGTTAATGGACTTTTGAAAGTGATTGTTGTGGGGGGAGGATTAGaaacaac                       >  1:7247849/1‑145 (MQ=12)

                                                                                                                              cgCGGCAACGCTCTCGCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCTCA‑TGGGCAGACTATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                       <  2:6257302/149‑1 (MQ=255)

                                                                                                                                cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGt                                                                       >  2:2866983/1‑100 (MQ=255)

                                                                                                                                cGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGt                                                                       <  1:2971193/100‑1 (MQ=255)

                                                                                                                                 ggCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCGTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                    <  1:6386137/150‑1 (MQ=255)

                                                                                                                                  gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGtt                                                                      <  2:3794065/99‑1 (MQ=255)

                                                                                                                                    aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTc                 >  2:2042679/1‑150 (MQ=255)

                                                                                                                                       gcactctcGCTTGCGCTTGAGTCGAACCTTGGTCTTTGCTTCTCTTCCTTCCCCGCA‑TGTGCAGAATATTTAATTGCGGATTCGTTTGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt              <  1:5045014/147‑1 (MQ=17)

                                                                                                                                       gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑Tggg                                                                                                       <  2:7817067/61‑1 (MQ=40)

                                                                                                                                         tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTTGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAGAAACTTCGAAGACGATGa            >  2:2425467/1‑150 (MQ=255)

                                                                                                                                         tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa            <  1:2425467/150‑1 (MQ=255)

                                                                                                                                         tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGa            <  1:2888730/150‑1 (MQ=255)

                                                                                                                                         tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGAGGCGTGGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCGTCGAAGTCGATGa            >  2:8748728/1‑150 (MQ=255)

                                                                                                                                         tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                 >  1:7651370/1‑129 (MQ=255)

                                                                                                                                         tctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAt                                 <  2:7651370/129‑1 (MQ=255)

                                                                                                                                           tctcGCGGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg          <  1:1770438/150‑1 (MQ=255)

                                                                                                                                            ctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACATCCCCCCA‑TGGGCATAATATTTAATTGCGGATTAGTTTGGAAGTTAAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAACTTCGAAGTAGATGActg         >  1:7348044/1‑148 (MQ=17)

                                                                                                                                            ctcGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTCTAATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGGAGTTAAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTCGAAACTTCGAAGTCGATGACgg         >  1:100615/1‑150 (MQ=18)

                                                                                                                                            ctcGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑Tggg                                                                                                       <  1:4201460/56‑1 (MQ=255)

                                                                                                                                            ctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                   >  2:7042994/1‑108 (MQ=255)

                                                                                                                                             tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGc        >  2:4793207/1‑150 (MQ=255)

                                                                                                                                              cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa       <  1:2162299/150‑1 (MQ=255)

                                                                                                                                              cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa       <  2:2776235/150‑1 (MQ=255)

                                                                                                                                               gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg      <  1:3709046/150‑1 (MQ=255)

                                                                                                                                                  ggCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAtt   >  1:6604750/1‑150 (MQ=255)

                                                                                                                                                   gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGtt                                                                              <  2:4074888/74‑1 (MQ=21)

                                                                                                                                                   gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGtt                                                                              >  1:4074888/1‑74 (MQ=21)

CATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCA‑TGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTT  >  NC_000913/1286996‑1287292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`