Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	699,468	A→C	6.5%	intergenic (‑291/+106)	asnB ← / ← umpH	asparagine synthetase B/UMP phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	699,468	0	A	C	6.5%	177.0 / 5.2	63	intergenic (‑291/+106)	asnB/umpH	asparagine synthetase B/UMP phosphatase
Reads supporting (aligned to +/- strand):  ref base A (21/35);  new base C (2/2);  total (23/40)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

GATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGG  >  NC_000913/699329‑699603                                                                                                                                             |                                                                                                                                        gATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCTAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGa                                                                                                                                         <  1:727119/140‑1 (MQ=255) gATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGa                                                                                                                                         <  1:90585/140‑1 (MQ=255) gATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGa                                                                                                                                         <  1:484953/140‑1 (MQ=255) gATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGa                                                                                                                                         <  1:484338/140‑1 (MQ=255)   tatatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAACAGCATGaaa                                                                                                                                       <  2:1047471/140‑1 (MQ=255)   tatatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGtaa                                                                                                                                       <  2:1005954/140‑1 (MQ=255)   tatatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaa                                                                                                                                       <  1:1159497/140‑1 (MQ=255)    atatTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGaaaa                                                                                                                                      >  2:495496/1‑140 (MQ=255)     tatttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAt                                                                                                                                     >  1:921992/1‑140 (MQ=255)     tatttattGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAAt                                                                                                                                     >  1:921452/1‑140 (MQ=255)           ttGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAata                                                                                                                               >  2:267661/1‑140 (MQ=255)           ttGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCAAGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACCAAAGCATGAAATTTCAata                                                                                                                               >  2:615545/1‑140 (MQ=255)            tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAatat                                                                                                                              >  1:589890/1‑140 (MQ=255)            tgatgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATAGCTGTCAATGTGCGATTTGTTCATGCCGGATGTGGCGTGAACGACTTATCCGGCCTACAAATGCTTGAAAATTCAagat                                                                                                                              >  2:992013/1‑140 (MQ=37)              ctgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAACGCATGAAAATTCAatatat                                                                                                                            <  1:329920/139‑1 (MQ=255)              atgaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAatatat                                                                                                                            <  1:331551/140‑1 (MQ=255)                gaATCGATAATTTTTAGCGGTATTTATTGACTGATAATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATTCCGGATGCGGAGTGAACGCCTTATCCCGCCTACAAAAGCATGAAAATTCAATAAATTg                                                                                                                          <  1:499323/140‑1 (MQ=37)                gaATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATAAATTg                                                                                                                          <  1:499348/140‑1 (MQ=255)                 aaTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                                         <  1:793652/140‑1 (MQ=21)                 aaTCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAGTCGTATAAATTCAATGTATTGc                                                                                                                         <  1:793541/140‑1 (MQ=21)                   tCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAg                                                                                                                       >  2:1014323/1‑140 (MQ=255)                    cGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAgg                                                                                                                      <  1:797079/140‑1 (MQ=255)                    cGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAgg                                                                                                                      <  1:676703/140‑1 (MQ=255)                    cGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGACAAAGTGCTGACTAAGTGCGATTTGTTCTTGCCGGATGCGGCGTGAACGCCTTATCCGGGCTACAAAAGCATGTTAATTCAATATATTGAAgg                                                                                                                      <  2:611712/140‑1 (MQ=25)                     gATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGa                                                                                                                     <  2:304543/140‑1 (MQ=255)                     gATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGa                                                                                                                     <  1:566175/140‑1 (MQ=255)                      aaaaTTTTAAGCGGGTTTTATTGAATGTTGATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTTCAGGAg                                                                                                                    <  2:1156564/138‑1 (MQ=255)                      aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                                                                                                    <  1:1232814/140‑1 (MQ=255)                      aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                                                                                                    <  1:1088891/140‑1 (MQ=255)                      aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                                                                                                    <  1:103947/140‑1 (MQ=255)                      aTAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAg                                                                                                                    <  2:1150650/140‑1 (MQ=255)                       tAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGc                                                                                                                         <  1:746012/134‑1 (MQ=18)                       tAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGCGAACGCCTTATCCGGCCTACAAAAGTTTGCAAATTCAATAAATTGc                                                                                                                         >  2:746012/1‑134 (MQ=18)                           ttttAGCGGGTTTTATTGCATGTTTCTATTTTCC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCg                                                                                                               <  2:537123/140‑1 (MQ=255)                               aGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTAATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAgg                                                                                                           <  2:1122045/140‑1 (MQ=255)                                gagggTTTTATTGAATGATTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGAATGAAAATTCAATATATTGCAGGAGCTGCGTAGGc                                                                                                          <  2:1004016/138‑1 (MQ=255)                                gCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGc                                                                                                          <  1:121060/140‑1 (MQ=255)                                     ttttATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGAt                                                                                                     <  1:1138223/140‑1 (MQ=255)                                     ttttATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGAt                                                                                                     <  2:518205/140‑1 (MQ=255)                                       ttATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATaa                                                                                                   <  2:508327/140‑1 (MQ=255)                                        tATTGACTGTTTAGCTTTTCC‑GGGGGCCAAATTGCTGACAAAGTGCGATTGGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGCTAAg                                                                                                  <  2:766901/140‑1 (MQ=21)                                             aaTGTTTATATTTTACGGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTa                                                                                              <  2:652594/140‑1 (MQ=255)                                             aaTGTTTATATTTTACGGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTa                                                                                              <  2:652447/140‑1 (MQ=255)                                                   tataTTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc                                                                                       <  1:542431/140‑1 (MQ=255)                                                     tatTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAg                                                                                     <  1:989975/140‑1 (MQ=255)                                                          tAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGt                                                                                >  1:1172546/1‑140 (MQ=255)                                                           aC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGtt                                                                               >  1:194278/1‑140 (MQ=255)                                                                        ttGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGGAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGtca                                                                   <  2:1056261/140‑1 (MQ=255)                                                                        ttGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGtca                                                                   <  1:1021180/140‑1 (MQ=255)                                                                        ttGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGtca                                                                   <  2:486235/140‑1 (MQ=255)                                                                        ttGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGtca                                                                   <  1:495496/140‑1 (MQ=255)                                                                            tGACAAAGTGCGCTTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCag                                                               >  2:696058/1‑140 (MQ=255)                                                                            tGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCag                                                               >  2:120483/1‑140 (MQ=255)                                                                                   gTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAAc                                                        >  2:977756/1‑140 (MQ=255)                                                                                   gTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAAc                                                        >  2:1139717/1‑140 (MQ=255)                                                                                      cGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTATCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCAc                                                     <  2:807724/140‑1 (MQ=255)                                                                                      cGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCAc                                                     <  2:807749/140‑1 (MQ=255)                                                                                      cGATTTGTACATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCAc                                                     <  2:928941/140‑1 (MQ=255)                                                                                                  gCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGATTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGt                                         <  1:477952/140‑1 (MQ=255)                                                                                                     ggATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGt                                      <  2:431462/140‑1 (MQ=255)                                                                                                      gATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc                                                                                       <  2:1005154/90‑1 (MQ=14)                                                                                                      gATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCACGCAAACTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATc                                                                                       >  1:1005154/1‑90 (MQ=14)                                                                                                                  aCGCTTTCTCCGGCCTACAACACCTTGAAAATTCAATTGATTGCAGGAGCGGCTTAGGCCTGATATGCGTAGCGCATCAGGGAGTTTGGCGTTTGTGATCTGAGCCATCCACGTCCGCAGACGTGGTTGCTTTTCAGATa                         <  2:851090/140‑1 (MQ=255)                                                                                                                               ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAg            >  1:1091765/1‑140 (MQ=255)                                                                                                                               ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAg            >  1:1187177/1‑140 (MQ=255)                                                                                                                               ccTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAg            >  1:200401/1‑140 (MQ=255)                                                                                                                                cTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGc           >  1:564422/1‑140 (MQ=255)                                                                                                                                         cATGAAAATTCTAGATATTGCAGGAGCTGCGTAGGCCTGATCAGCGTAGCGCATCTGGCGGTTTGGCGGTTGGCATCAGAGCCATCCACGGCCGCCGCCGGGGGTGCTATTCAGAAACCGTCGAGGTCAGCGACGGTCgg  >  2:405226/1‑140 (MQ=255)                                                                                                                                         cATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACgg  >  2:834960/1‑140 (MQ=255)                                                                                                                                         cATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACgg  >  2:566441/1‑140 (MQ=255)                                                                                                                                         cATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACgg  >  2:208484/1‑140 (MQ=255)                                                                                                                                             |                                                                                                                                        GATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTAC‑GGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAAAGCATGAAAATTCAATATATTGCAGGAGCTGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTTTGGCGTTTGTCATCAGAGCCAACCACGTCCGCAGACGTGGTTGCTATTCAGATAACGTCGATTTCAGCGACTGACGG  >  NC_000913/699329‑699603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑