| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,229,177 | A→C | 12.8% | intergenic (+112/+261) | yohP → / ← dusC | uncharacterized protein/tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,229,177 | 0 | A | C | 12.8% | 366.7 / 12.3 | 174 | intergenic (+112/+261) | yohP/dusC | uncharacterized protein/tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base A (40/95); new base C (21/4); total (64/110) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 180 total aligned reads displayed.
TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG > NC_000913/2229028‑2229326 | aCTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACTAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAg < 1:1900238/150‑1 (MQ=255) aCTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAg < 2:276006/150‑1 (MQ=255) tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAggg < 1:1453170/150‑1 (MQ=255) tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAggg < 2:1136905/150‑1 (MQ=255) tggtggTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAggg < 2:2884726/150‑1 (MQ=255) ggtggtGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCgggg > 2:4753655/1‑150 (MQ=255) gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCggggg > 2:1027142/1‑150 (MQ=255) gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAggggg > 1:4484999/1‑150 (MQ=255) gtggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAggggg < 1:3190664/150‑1 (MQ=255) tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGggc > 1:4055098/1‑150 (MQ=255) tggtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggc < 1:1488527/150‑1 (MQ=255) tggtcACTGGCGTATTTAAGATGATATTATAGAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGGCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCggggggg > 1:4547573/1‑149 (MQ=255) gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGggcgg > 1:5968742/1‑150 (MQ=255) gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcgg < 1:2054920/150‑1 (MQ=255) gtgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcgg < 1:479888/150‑1 (MQ=255) tgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcggc < 2:3868726/150‑1 (MQ=255) tgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcggc < 2:5735918/150‑1 (MQ=255) tgACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcggc < 2:2486677/150‑1 (MQ=255) gACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGcc < 2:3727125/150‑1 (MQ=255) aCTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGC‑GCCCGGGGGGGccc > 2:4634430/1‑149 (MQ=255) tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGggcgggccaa > 2:1281644/1‑145 (MQ=255) tGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAt < 2:5319933/150‑1 (MQ=255) gCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAcg > 2:3018941/1‑150 (MQ=255) aTTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccgc < 1:902142/150‑1 (MQ=255) tttAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccgcc < 2:3542479/150‑1 (MQ=255) tAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccgccgc < 2:2653268/150‑1 (MQ=255) aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGccc < 2:2069877/150‑1 (MQ=255) aGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGccc < 1:287762/150‑1 (MQ=255) gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCGcccc > 1:1002125/1‑150 (MQ=255) atgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGccccc < 1:4282985/150‑1 (MQ=255) tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCt < 2:953692/150‑1 (MQ=255) tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGTGGCCCATCGCCGCCGCCCCCt > 1:1845075/1‑150 (MQ=255) tgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGTGGCCAAACGCCGCCGccccca > 1:1844982/1‑149 (MQ=255) gatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTg < 2:4066897/150‑1 (MQ=255) atatTTTAAAATTAATTAATGTCATCTGGTCCGAAAATAACGAGAATATTTCTGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCATGGAGGGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGc < 1:5891913/150‑1 (MQ=38) tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGGCCAAAGCCCCCCCCCCCCGCc‑ccc > 1:5255778/1‑150 (MQ=255) tAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGGGCAAAAGCCCCCCCCCCCCGCc‑ccccc > 2:5200161/1‑150 (MQ=255) tAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCGGCA‑ccccc > 2:3928162/1‑150 (MQ=255) attaattaATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCAGCA‑CCCCCCGGc > 2:4415368/1‑150 (MQ=255) ttaattaaTGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCCCCCCCGGCA‑CCCCCCGGca > 2:5903814/1‑149 (MQ=38) taatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCACTGGc > 2:4007537/1‑150 (MQ=255) tGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGaa < 2:3612396/150‑1 (MQ=255) tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGGCCAACGCCCCCCCCCCCCCCC‑CCCCCCCGCCCGGGCGGACa > 2:83440/1‑150 (MQ=255) tcaGGTCCGACACTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACaaaa < 2:5582464/150‑1 (MQ=255) tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACaaaa < 2:262907/150‑1 (MQ=255) tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCCCCCCCAGCA‑CCCCCCCGCGCTGGCGAACaaaa > 2:1521155/1‑150 (MQ=25) ggTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCCGGGCTCTGGCGAACAAAATc < 2:673145/150‑1 (MQ=255) ggTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCCGGGCTCTGGCGAACAAAATc < 2:673005/150‑1 (MQ=255) tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGGCCCAACGCCCCCCCCCCCAGCA‑CCCCCCGCCGCAGGGGAaaaaaaaagcc > 1:3425282/1‑145 (MQ=255) ccGAAAATAACGAGAATATTTAAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGTGGGCCGCCCAAAGCCGCCCCCCCCGGCA‑CCCCCCGGCGCTTGGGAACaaaaaaaccc > 2:4210586/1‑144 (MQ=255) cgcacaTAACGCGAATATTTCAGACCCTCACCCTGTTGCGCCCCTGCCATGTGCATGGCTTCATACAAGCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCTGCC‑CCCCCGGGCTCTGGCTAACAAAATcgccgc < 2:232622/145‑1 (MQ=18) cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑AGCCCCCCCCCCCCCCC‑CCCCCCCGCCCGGGCGCACaaaaaaacccc > 2:5612696/1‑143 (MQ=255) cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGGGCACAAGGCCCCCCCCCCAGCC‑CCCCCCCGCACGGGGGAACaaaaaaaaccc > 1:1803877/1‑143 (MQ=255) aaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCGATTGgccgccccc < 2:1868271/109‑1 (MQ=37) aTAACGATAATATTTCTGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGATTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTgcgc < 2:1045701/150‑1 (MQ=255) aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGt < 2:3691708/150‑1 (MQ=255) agaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACCCCCCCGCCCCCCGCA‑CCCCCCGGCACAGGCCAACAAAAAACCCCCAGCGCGGTGcc > 2:3324287/1‑150 (MQ=21) gaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCCCCGCCCCCGGCA‑CCCCCCGGCTCTGGCGAACAAAAAAGCCGCGGCGCGGTGccc > 2:1008466/1‑150 (MQ=21) gaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCCGCA‑CCCCCCGGCTCAGGCGAACAAAATAGCCGCGGCGCGGTGccc > 2:1008656/1‑150 (MQ=21) gaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCCGGCTCTGGCGAACAAAATAGCCGCTGCGCGGTGccc > 2:3969956/1‑150 (MQ=255) atatTTCAGCCTCCCATCCTGTGGCGCTCCTGTCATGTGCAGGGCTTCATATACGCCCGGGCGCACGGCGCGC‑GCCGGGGGCGGCCAAGCGCCGCCGCCCCCGGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTc < 1:3317508/150‑1 (MQ=17) tatTTCAGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCg < 2:1537803/150‑1 (MQ=255) tatTTCAGTCTCTCATCCTGTTGCGCTCCGGTCATGTGCTTTGCTTCATATAATCCCTGGCGCACGGCGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACACAATCGCCGCTGCGCGGTGCCCTCg < 2:5640809/150‑1 (MQ=18) aGTCTCTCTTCCTGTTGCGCTCCTTTCTTGTGCTTTGCTAAATATAACCACTGGCGAACGGCGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTAt < 1:753419/150‑1 (MQ=14) tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCCAACGCCCCCCCCCCCAGCA‑CCCCCCGGCTCAGGCGAACAAAAAACCCGCGGCGCCGGGGCCCCGGCGTATacca > 2:3189470/1‑149 (MQ=18) cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAc < 2:3359822/150‑1 (MQ=255) agccTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACg < 2:5266520/148‑1 (MQ=255) gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc < 2:4021149/150‑1 (MQ=255) gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAc < 2:4250418/150‑1 (MQ=255) gcgcgcCGGTCTTGTGCTTTGCTTCTTCTCATCACGGGGGCAAGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGGTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCGTCGGCTTATCCCTTACGGGTACCgg < 2:4547573/150‑1 (MQ=14) cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCGGCCCAACGCCGCCGCCCCCCGCA‑CCCCCCCGCGCAGGCGAACAAAATACCCCCAGCCCGGGGGCCTCGGCGTATCCCAAACGGcgaacccg > 2:3810922/1‑143 (MQ=12) cTCCTGTCTTGTGCTTTGCTTCATATAATCACTGGCGCAAGGTGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc < 1:1947278/150‑1 (MQ=255) cTGTCATGTGCATTGCTTCATATAATCACGGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg < 1:1497582/150‑1 (MQ=255) aTGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa < 2:1672819/150‑1 (MQ=255) aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGGCCAAAACCCCCCCCCCCCCGCA‑CCCCCCCGCGCAGGCCAACAAAAAACCCCCAGCGCGGGGCCCGCCGCAGATACCGTAACGCTAACCGGTCCGGcgcgc > 2:4886712/1‑149 (MQ=255) tgtgCATTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg < 2:2238259/150‑1 (MQ=255) tgtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg < 2:5417794/150‑1 (MQ=255) cATTGCTTCATATAATCACTGGCGCAAGTAGCGC‑GCAGGGGGCGGCCGATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa < 1:4925044/150‑1 (MQ=255) cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa < 2:1471098/150‑1 (MQ=255) cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCACGGGGCCGCCAAAAGCCGCCCCCCCCAGCC‑ACCCCCGGCTCGGGCCCACAAAAACGCCCCAGCGCGGGGGCCGCCGCGGATACCATACCGCCGCCCGGTCGGGGGCGGGGGaa > 2:5375593/1‑150 (MQ=9) cATTGCTTCATAGAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCACTCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACCAACTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa < 2:4863553/150‑1 (MQ=255) cTTCATATAATCACTGGCGCATGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc < 1:3516324/150‑1 (MQ=255) cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc < 1:4415368/150‑1 (MQ=40) ttCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAAGCCCCCGCCCCCAGCA‑CCCCCCGGCGCAGGCGGACAAAAAACCCGCGGCCCCGTGCCCACCGCATATCCCATAACGCAAACCGGTCGGGCGCGCGGGAACaaacc > 2:4917409/1‑146 (MQ=255) tCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCt < 1:472204/150‑1 (MQ=39) atatAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt < 1:3788394/150‑1 (MQ=38) atatAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGt < 1:3788027/150‑1 (MQ=38) tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTa < 2:4720722/150‑1 (MQ=37) tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg < 2:601340/150‑1 (MQ=32) cACTGGCGCAAGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcgc < 1:5225787/150‑1 (MQ=17) gcgcAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt < 1:5518847/150‑1 (MQ=32) cAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAg < 2:5145860/150‑1 (MQ=32) tggAGCGC‑GCTGGGGGCGGCAAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc < 1:5242977/149‑1 (MQ=17) gc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc > 2:5048764/1‑150 (MQ=32) gc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc > 2:776827/1‑150 (MQ=32) gc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc > 2:4988362/1‑150 (MQ=32) cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt < 2:6424/150‑1 (MQ=32) cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt < 2:4814034/150‑1 (MQ=32) aGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGATACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg > 2:5409755/1‑150 (MQ=17) aGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCCCCTCCAtg > 2:2661607/1‑150 (MQ=255) | TACTGGTGGTGACTGGCGTATTTAAGATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCA‑CCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATG > NC_000913/2229028‑2229326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |