BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,648,128	G→T	6.1%	noncoding (66/84 nt)	agrA →	inactive antisense sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,648,128	0	G	T	6.1%	355.9 / 7.6	128	noncoding (66/84 nt)	agrA	inactive antisense sRNA
Reads supporting (aligned to +/- strand): ref base G (49/70); new base T (5/4); total (54/74)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTG  >  NC_000913/3647980‑3648275

aaCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGc                                                                                                                                                    >  2:1009628/1‑150 (MQ=255)

     gATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGt                                                                                                                                               >  1:148183/1‑150 (MQ=255)

        aaTCATACAGTACATGCAGGTTATAAAACCAGCAAGTCCTTGCAATAGTTTCAGTTTGGTTTTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCTGAGCCACCAACTCAGGGCTTGAAAGT‑AAAAAACCGACGCAAAGTCgg                                                                                                                                            <  1:1765188/150‑1 (MQ=38)

         aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGt                                                                                                                                           >  1:725518/1‑150 (MQ=255)

            aTACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttt                                                                                                                                        <  1:948672/150‑1 (MQ=255)

              aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                      <  1:1017502/150‑1 (MQ=255)

              aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                      <  2:1660951/150‑1 (MQ=255)

               cAGTACATGCAGTTTATAAAACCAGCACGTCCTTGCAAAAGTTTCAGTATGTTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTGAGAGCCACCAACTCAGTGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                     <  2:249278/150‑1 (MQ=37)

               cAGTACATGCAGGTTCTAAAACCAGCACGGCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                     <  2:1139596/150‑1 (MQ=255)

               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                     >  1:2124538/1‑150 (MQ=255)

                aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGA‑AAAAAACCGACGCAAAGTCGGtttttatt                                                                                                                                    >  2:1878783/1‑147 (MQ=255)

                aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGAGGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑TAAAAACCGACTTTGCGTCGGttttttac                                                                                                                                    <  1:653630/150‑3 (MQ=37)

                 gTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAACCGACTTTGCGTCGGttttttac                                                                                                                                    <  2:990900/150‑3 (MQ=38)

                    cgtGCTGGTTTTATAACCTGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAAGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATc                                                                                                                                >  2:1937197/3‑150 (MQ=37)

                     aTGCAGTTTATAAAACCAGCACGTCCTTGCAAAAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAACCGACTTTGCGTCGGttttttactttc                                                                                                                                <  1:2115433/150‑7 (MQ=37)

                        cAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGa                                                                                                                            >  2:1709258/1‑150 (MQ=255)

                        cAGGTTATAAAACCAGCACCTCCTTGCAATTCTATCAGTAAGGAATTAGCATTGAAGCGTAAGATTATGGCTAAAACAATCCAGACAAATCCCCCAACACAGCGATGGAAAGA‑AAAAAACCGACGAAAAGTCCATTTTTATAcgacctca                                                                                                                            >  2:422972/1‑143 (MQ=255)

                         aGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAt                                                                                                                           <  2:28824/150‑1 (MQ=255)

                         aGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAt                                                                                                                           <  1:994643/150‑1 (MQ=255)

                         aGGTTATAAAACCAGCACGTCCTTGCAAAAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCACAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAt                                                                                                                           <  1:2002170/150‑1 (MQ=255)

                          ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACAGACTAAAAGTCGTTTTTTTTACATCCGTAta                                                                                                                          >  2:341832/1‑149 (MQ=37)

                             tataAAACCAGCACGTCCTTGCAATAGTTTCACTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCgg                                                                                                                       <  2:1261840/150‑1 (MQ=255)

                               taAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAGATCCGGagtgggcc                                                                                                                     >  1:2075551/1‑143 (MQ=255)

                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                    >  2:389371/1‑150 (MQ=255)

                                     cAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCt                                                                                                               <  2:86759/150‑1 (MQ=255)

                                          cGTCATTGCAATAGTATCAGTATGGGATTAGCATTGATGCGGTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCACCTCAGGGCTGGTAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                          <  2:326083/150‑1 (MQ=25)

                                           gTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAatat                                                                                                         <  2:470653/150‑1 (MQ=255)

                                             ccTTGCAATAGTTTCATTATGGTATTAGCATTGTTTCGTTAGATGATGGCTATCTCAATCCAGTCCGAGCCAACAACTCAGGGATTGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGa                                                                                                       <  1:214649/150‑1 (MQ=37)

                                             ccTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGa                                                                                                       <  1:1949781/150‑1 (MQ=255)

                                             ccTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGa                                                                                                       >  2:890261/1‑150 (MQ=255)

                                              cTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACACCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGAc                                                                                                      <  1:779244/150‑1 (MQ=255)

                                                tGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGa                                                                                                    >  2:1893380/1‑150 (MQ=255)

                                                  cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCTGATTCAGACATCCATTAATATGACGaag                                                                                                  >  1:932870/1‑148 (MQ=18)

                                                  cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATg                                                                                                  <  2:42412/150‑1 (MQ=255)

                                                  cAATAGTTTCAGTATGGTATTAGCATTGATGAGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGTCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATACGGAAAAGCTTTAAAATGACGATg                                                                                                  >  1:1077628/1‑150 (MQ=25)

                                                   aaTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGa                                                                                                 <  1:408007/150‑1 (MQ=255)

                                                     tAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAcc                                                                                               <  1:1491912/150‑1 (MQ=255)

                                                          tCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTg                                                                                          <  2:2009262/150‑1 (MQ=255)

                                                          tCAGGATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTg                                                                                          <  1:374794/150‑1 (MQ=255)

                                                             gTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaaa                                                                                       >  1:1140954/1‑150 (MQ=255)

                                                              tATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAg                                                                                      <  1:511063/150‑1 (MQ=255)

                                                                  gTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtata                                                                                  <  2:954791/150‑1 (MQ=255)

                                                                   tATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatat                                                                                 <  2:537915/150‑1 (MQ=255)

                                                                    tttatctttGATGCGTTAGATTATCGCTATCTCACTGCAGTCTTAGCCACCATCTCTGGGGTGGAATGT‑TATAAACCGACGGAAAGTCTGTTTTTTTACATCCGGATTCGGACATGGCTTAATTTTACGATGACCCAGTGAATGtatata                                                                                <  1:140210/143‑1 (MQ=255)

                                                                    aTTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTGTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                >  2:766101/1‑150 (MQ=255)

                                                                         cATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCg                                                                           <  2:864677/150‑1 (MQ=255)

                                                                           ttGATGCGTTAGATGATGGCTATCTCACACCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGAAGCAAAGTCGGTTTTTTTACTTCCTGATTCGGAAAAGGCTTAATATTAATATGACACACTGAAAGAAAATAAATcagc                                                                         >  1:1606889/1‑147 (MQ=18)

                                                                            tGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCa                                                                        >  2:2116395/1‑150 (MQ=255)

                                                                             gATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACAGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACATGGATTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCAc                                                                       >  1:1035676/1‑150 (MQ=255)

                                                                              aTGCGTTAGATGATTTCTATCTCAATCCAGTCAGAGCCAACAACTCAGGTCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACt                                                                      <  1:341832/150‑1 (MQ=37)

                                                                              aTGCGTTAGATGATGTCTATCTCACTCCAGTCAGATCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACt                                                                      <  2:1038193/150‑1 (MQ=255)

                                                                                  gTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGa                                                                  <  1:2045984/150‑1 (MQ=255)

                                                                                   ttAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGat                                                                 <  1:2116395/150‑1 (MQ=255)

                                                                                   ttAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGat                                                                 <  2:148183/150‑1 (MQ=255)

                                                                                   ttAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCACGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGat                                                                 <  1:301838/150‑1 (MQ=255)

                                                                                    tAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGTAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATCTGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGata                                                                <  1:372398/150‑1 (MQ=255)

                                                                                     aGATGATGGCTATCTCCCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTATTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGatat                                                               >  1:1361417/1‑150 (MQ=255)

                                                                                       atgatgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGatatat                                                             >  2:954297/1‑150 (MQ=255)

                                                                                          atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAcc                                                          <  2:1838221/150‑1 (MQ=255)

                                                                                             gCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAATT‑AAAAAACCGACGCAAAGTCGG‑TTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATATATCGTCACTGCGATATATACCGAAg                                                      <  1:1568561/150‑1 (MQ=255)

                                                                                             gCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGaa                                                       >  2:1372036/1‑150 (MQ=255)

                                                                                             gCTATCTCACTCCAGTCACAGCCACCAACTCAGGGCTGGCAAGT‑AAAAACCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGaa                                                       <  2:1369400/150‑1 (MQ=255)

                                                                                                 tctcACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGc                                                   >  2:1123719/1‑150 (MQ=255)

                                                                                                     aCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccc                                               <  1:1577907/150‑1 (MQ=255)

                                                                                                        ccAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccctcc                                            <  1:1950489/150‑1 (MQ=255)

                                                                                                         cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                           <  1:1250380/150‑1 (MQ=255)

                                                                                                               gagCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCt                                     <  1:1803525/150‑1 (MQ=255)

                                                                                                                 gCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACCTCCGGATTCGGACATGGCTTATTAAGACGATGACCGAGTGAAAGTATATAAATCGTGACTGAGATATATCCCGAAGTGCTCCCTCCGCCAGCTgg                                   >  2:1994428/1‑149 (MQ=25)

                                                                                                                   caccaACTCAGTGATTGAAATT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGATAGTATATAAATCTTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                 <  1:1435485/150‑1 (MQ=38)

                                                                                                                   caccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGTCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGTTATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                 <  1:535166/150‑1 (MQ=255)

                                                                                                                   caccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                 >  1:1318130/1‑150 (MQ=255)

                                                                                                                   aactaaCTCAGGTATGGAAAGT‑ATAAAACCGACTCAAAGTAGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaag                                 <  1:292728/146‑1 (MQ=255)

                                                                                                                     ccaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaagaa                               <  2:123184/150‑1 (MQ=255)

                                                                                                                        aCTCAGGGCTTGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGTCCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATc                            <  1:801457/150‑1 (MQ=255)

                                                                                                                        aCTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACTAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGGGCTCCCTCCGCCAGCTGAAGAAATc                            <  2:794311/150‑1 (MQ=255)

                                                                                                                         cTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                           >  2:737317/1‑150 (MQ=255)

                                                                                                                           cAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCt                         <  1:1223837/150‑1 (MQ=255)

                                                                                                                             gggCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGTAATCGATaa                       >  2:743581/1‑150 (MQ=255)

                                                                                                                             gggCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGAACCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGGGCTCCCTCCGCCATCTGAAGAAATCGCTaa                       >  2:1059465/1‑150 (MQ=255)

                                                                                                                                cTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTc                    <  1:1783464/150‑1 (MQ=255)

                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                  >  2:1361174/1‑150 (MQ=255)

                                                                                                                                    gatgtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGAGTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCGCTGCGATTTATTCCGTAGTGCTCCCTCCGCCAGCTGAAGAAATGGCTAATTCTTg                 >  1:1308317/7‑150 (MQ=255)

                                                                                                                                    gatgtaaaaaaaCCGACTTTGCGTCGG‑TTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                >  1:1192199/7‑150 (MQ=255)

                                                                                                                                    aaaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                <  2:1216837/150‑1 (MQ=255)

                                                                                                                                    aaaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                <  1:752152/150‑1 (MQ=255)

                                                                                                                                    aaaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                <  1:1937197/150‑1 (MQ=255)

                                                                                                                                     aaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa               <  1:389371/150‑1 (MQ=255)

                                                                                                                                     aaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa               >  2:633089/1‑150 (MQ=255)

                                                                                                                                     aaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa               <  2:725518/150‑1 (MQ=255)

                                                                                                                                       gT‑AGAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt             >  1:791803/1‑150 (MQ=255)

                                                                                                                                       gT‑AAAAAACCGACGCAAAGTCGGTTATTTTACATCCGGATTCGGACAAGCCTTAATATTACGATGACCCAGTGAAAGTATATAAATCGTCAATGCGATATATACCGAAGTGCTGCCTCCGCCAGCTGATGAAATAGCTAATTCTTGCAAt             >  2:1850721/1‑150 (MQ=255)

                                                                                                                                        taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGCCCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt             >  1:1899136/3‑150 (MQ=255)

                                                                                                                                        taaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAt             >  1:2106100/3‑150 (MQ=255)

                                                                                                                                        t‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAAtt            >  2:2199612/1‑149 (MQ=255)

                                                                                                                                          aaaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGt           >  1:666297/1‑150 (MQ=255)

                                                                                                                                          aaaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGt           >  2:1771026/1‑150 (MQ=255)

                                                                                                                                           aaaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGtt          >  2:824355/1‑150 (MQ=255)

                                                                                                                                               aCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGAGGATGCCCCAGTGAAAGTAAAAAAATCGTCGCTGAGTGATATAACTAAGTGCTACCTCAGCAATCTGAATGAATCGCTAATTAGTGCATTGtgtgcc      >  2:367376/1‑145 (MQ=255)

                                                                                                                                                ccGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCa     >  1:519485/1‑150 (MQ=255)

                                                                                                                                                   aCGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTg  <  1:1253231/150‑1 (MQ=255)

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTG  >  NC_000913/3647980‑3648275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`