BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,180,505	G→T	7.6%	E29* (GAG→TAG) ‡	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,505	0	G	T	7.6%	641.4 / 27.1	179	E29* (GAG→TAG) ‡	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand): ref base G (74/91); new base T (7/7); total (81/98)
Fisher's exact test for biased strand distribution p-value = 7.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

AAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCA  >  NC_000913/3180356‑3180652

aaacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCg                                                                                                                                                      >  2:1258438/1‑150 (MQ=255)

 aacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGa                                                                                                                                                     >  2:2823678/1‑150 (MQ=255)

 aacaaaGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGa                                                                                                                                                     >  1:1026093/1‑150 (MQ=255)

            aTGCAGCTTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTAcatggt                                                                                                                                         <  2:135275/150‑6 (MQ=255)

            aTGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgt                                                                                                                                         <  2:964700/150‑1 (MQ=255)

            aTGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgt                                                                                                                                         >  2:283901/1‑150 (MQ=255)

             tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtg                                                                                                                                        >  1:2339578/1‑150 (MQ=255)

             tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtg                                                                                                                                        >  1:1449186/1‑150 (MQ=255)

             tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTACATGgtg                                                                                                                                        <  1:1595810/150‑1 (MQ=255)

              gCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAA‑TGgtgt                                                                                                                                       <  2:863514/150‑1 (MQ=255)

               cAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGtgtg                                                                                                                                      >  2:2514051/1‑150 (MQ=255)

                    tAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGAt                                                                                                                                 <  2:2266005/150‑1 (MQ=255)

                    tAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGAt                                                                                                                                 <  2:1435509/150‑1 (MQ=255)

                      gTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc                                                                                                                               >  1:923269/1‑150 (MQ=255)

                       tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATCTTCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAAAttga                                                                                                                              <  1:265775/150‑4 (MQ=38)

                       tGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATCTTCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAAAttga                                                                                                                              <  1:2156693/150‑4 (MQ=38)

                            ccGGTACCTCTTCTCGTTCAATGGGTGGCTGATTCCGATGGAAAGAGTCAGTATTACCGAGCGCCCTGACTGGCGTGAGAAAGCCCACGAATTCGGTTTCAATTTTCACACCTTGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtacta                                                                                                                         <  2:501164/150‑1 (MQ=255)

                            ccGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtacta                                                                                                                         >  2:404357/1‑150 (MQ=255)

                             cGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtactac                                                                                                                        >  1:280274/1‑150 (MQ=255)

                             cGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtactac                                                                                                                        >  2:1530105/1‑150 (MQ=255)

                               gTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACaa                                                                                                                      <  1:1835467/150‑1 (MQ=255)

                               gTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACaa                                                                                                                      <  1:1300148/150‑1 (MQ=255)

                                    tcttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGa                                                                                                                 >  1:2018740/1‑150 (MQ=255)

                                    tcttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGa                                                                                                                 >  2:2595441/1‑150 (MQ=255)

                                    tcttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGa                                                                                                                 <  2:1236582/150‑1 (MQ=255)

                                    tcttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGa                                                                                                                 >  2:2247082/1‑150 (MQ=255)

                                     cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAc                                                                                                                <  1:704227/150‑1 (MQ=255)

                                     cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAc                                                                                                                <  2:1267059/150‑1 (MQ=255)

                                      ttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAcc                                                                                                               <  1:13755/150‑1 (MQ=255)

                                      ttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAcc                                                                                                               >  2:1065922/1‑150 (MQ=255)

                                         tcGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTc                                                                                                            >  2:1805840/1‑150 (MQ=255)

                                         tcGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTc                                                                                                            <  1:44414/150‑1 (MQ=255)

                                            ttCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGcc                                                                                                         >  1:1233712/1‑150 (MQ=255)

                                            ttCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGcc                                                                                                         >  2:228479/1‑150 (MQ=255)

                                             tCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGccc                                                                                                        >  1:993329/1‑150 (MQ=255)

                                                     tGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaa                                                                                                <  1:503507/150‑1 (MQ=255)

                                                     tGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaa                                                                                                <  2:551910/150‑1 (MQ=255)

                                                         tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAAGTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTAGACCCGCGCCCAGGGTGAAAAGc                                                                                            >  1:1484413/1‑150 (MQ=255)

                                                         tGATACCGAAGTAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTATCACACCATGAACGGCGAGCCGTAC‑TCGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGc                                                                                            >  2:2065/1‑150 (MQ=255)

                                                          gATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCt                                                                                           <  2:2122239/150‑1 (MQ=255)

                                                           aTACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTg                                                                                          >  1:1693631/1‑150 (MQ=255)

                                                             aCCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGga                                                                                        <  2:170273/150‑1 (MQ=255)

                                                              ccGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaa                                                                                       >  2:1913563/1‑150 (MQ=255)

                                                              ccGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaa                                                                                       >  2:2684945/1‑150 (MQ=255)

                                                                 aTGGAAAGAGTCAGTATTACCGAGCGCCCGGTCTGGCGTGAGAAAGCCCACGACTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                                    <  1:1203049/150‑1 (MQ=255)

                                                                 aTGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                                    <  2:459867/150‑1 (MQ=255)

                                                                 aTGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaagaa                                                                                    <  2:690417/150‑1 (MQ=255)

                                                                   ggAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGt                                                                                  >  2:72760/1‑150 (MQ=255)

                                                                    gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTc                                                                                 >  2:716113/1‑150 (MQ=255)

                                                                     aaaGAGTCAGTATTACCGAGCGCCCGGACTGGCGAGAGAAAGCCCACGAATACGGTATCAATTTTCACACCATGTACGGCGAGCCGTAC‑TCGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCa                                                                                <  2:565882/150‑1 (MQ=255)

                                                                     aaaGAGACAGAATTCCCGAGCGCCCGGACTGGCGTCAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TTGTGTGAAGATGCTTACTACACGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGCCa                                                                                <  1:2436256/150‑1 (MQ=255)

                                                                        gagTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACACGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccg                                                                             <  2:1686303/150‑1 (MQ=255)

                                                                        gagTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccg                                                                             <  2:1686206/150‑1 (MQ=255)

                                                                           tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgccg                                                                          >  2:2350707/1‑150 (MQ=255)

                                                                           tCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgccg                                                                          >  1:2746712/1‑150 (MQ=255)

                                                                             aGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGaa                                                                        <  1:2404804/150‑1 (MQ=255)

                                                                             aGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGaa                                                                        <  1:2277846/150‑1 (MQ=255)

                                                                                aTTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTg                                                                     <  2:1072916/150‑1 (MQ=255)

                                                                                aTTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTg                                                                     <  2:1244680/150‑1 (MQ=255)

                                                                                   aCCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAc                                                                  <  2:9234/150‑1 (MQ=255)

                                                                                     cgcgcgcCCGGACTGGCGTGAGAAAGCCCACGAATACGTTTTCATGTTTCACACCATGTCCGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCa                                                                <  1:242450/147‑1 (MQ=255)

                                                                                          gcCCGGACTGGCGTGAGAAAGCCCACGAATACGTTTACAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt                                                           <  1:2576511/150‑1 (MQ=255)

                                                                                          gcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt                                                           <  1:2581171/150‑1 (MQ=255)

                                                                                          gcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt                                                           <  2:2873099/150‑1 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAACGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                         >  1:2407960/1‑150 (MQ=255)

                                                                                            ccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                         >  1:1048854/1‑150 (MQ=255)

                                                                                            ccGGACGGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGc                                                         <  1:1345260/150‑1 (MQ=255)

                                                                                             cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGcc                                                        <  1:312244/150‑1 (MQ=255)

                                                                                               gACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTg                                                      >  1:2540662/1‑150 (MQ=255)

                                                                                                  tGGCGTGAGACAGCCCCCGAATCCGGTTTCAATTTGCACACCCTGTACGGCGAGCCGTAC‑GGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaaa                                                   <  1:2736472/150‑1 (MQ=255)

                                                                                                  tGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaaa                                                   >  1:2587492/1‑150 (MQ=255)

                                                                                                  tGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaaa                                                   <  1:310060/150‑1 (MQ=255)

                                                                                                     cGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtg                                                <  1:724285/150‑1 (MQ=255)

                                                                                                        gagaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtggtg                                             <  1:2313931/150‑1 (MQ=255)

                                                                                                         agaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGACAGtggtgg                                            >  2:628429/1‑150 (MQ=255)

                                                                                                          gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaa                                                                                       <  2:1173896/106‑1 (MQ=255)

                                                                                                          gaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGa                                           >  1:1034863/1‑150 (MQ=255)

                                                                                                           aaaGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGaa                                          >  2:2208624/1‑150 (MQ=255)

                                                                                                              gCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGaaaaa                                       <  2:2291587/150‑1 (MQ=255)

                                                                                                                   agAATACTGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGAt                                  <  1:1915362/149‑1 (MQ=255)

                                                                                                                    gAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATc                                 <  2:1869724/150‑1 (MQ=255)

                                                                                                                     aaTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTTCTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCg                                >  1:1803676/1‑150 (MQ=255)

                                                                                                                      aTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGc                               <  1:2193464/150‑1 (MQ=255)

                                                                                                                        aCGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCa                             >  2:264509/1‑150 (MQ=255)

                                                                                                                           gTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCg                          >  1:814556/1‑150 (MQ=255)

                                                                                                                                  ttttCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCt                   <  1:794805/150‑1 (MQ=255)

                                                                                                                                  tcttCACACCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTAATAAAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACAGCACCAGATGTGCCAGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCt                   >  1:1087518/3‑150 (MQ=38)

                                                                                                                                     tCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgat                <  1:1429073/150‑1 (MQ=255)

                                                                                                                                         acCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAcc            >  1:2785916/1‑150 (MQ=255)

                                                                                                                                          accagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAcc            >  2:1913826/5‑150 (MQ=255)

                                                                                                                                          accagTACGGCTCGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCa           >  2:1601961/5‑150 (MQ=255)

                                                                                                                                           ccagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCa           >  1:115359/4‑150 (MQ=255)

                                                                                                                                           cATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCaa          >  1:551200/1‑150 (MQ=255)

                                                                                                                                              gTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAAtt       <  1:2323891/150‑1 (MQ=255)

                                                                                                                                              gTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAAtt       >  2:2387794/1‑150 (MQ=255)

                                                                                                                                                aCGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTcc     <  1:1424274/150‑1 (MQ=255)

                                                                                                                                                 cGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg    <  2:713544/150‑1 (MQ=255)

                                                                                                                                                 cGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCg    <  1:2595441/150‑1 (MQ=255)

                                                                                                                                                  ggCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGc   >  2:1764136/1‑150 (MQ=255)

                                                                                                                                                   gCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCa  >  1:2496695/1‑150 (MQ=255)

AAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCA  >  NC_000913/3180356‑3180652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`