BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,180,513:1	+A	6.5%	coding (93/1161 nt)	ygiC →	ATP‑Grasp family ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,513	1	.	A	6.5%	407.0 / 23.9	108	coding (93/1161 nt)	ygiC	ATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand): ref base . (55/46); new base A (4/3); total (59/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.47e-01

Only 100 of 111 total aligned reads displayed.

ACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAAC  >  NC_000913/3180365‑3180661

aCTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑t                                                                                                                                                     <  2:346604/150‑1 (MQ=255)

  tATGCAGCGTAGTGCAACCGGTACCTCATCTCGTTCAATGGGTGGCTGATACCGAAGGAAAGAGTCAGTATTACCGCGCGCCCGTACTGGCGTGAGAAAGACCACGAATACGGTTTCAATTTTCACAAAATGTACGGCGAGCCGTAC‑tcg                                                                                                                                                   >  2:253990/1‑148 (MQ=255)

   aTGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgt                                                                                                                                                  >  1:1067305/1‑150 (MQ=255)

    tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGgtg                                                                                                                                                 >  1:1583558/1‑150 (MQ=255)

    tGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTACATGgtg                                                                                                                                                 <  2:1593602/150‑1 (MQ=255)

      cAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACAGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGtgtg                                                                                                                                               <  2:228981/150‑1 (MQ=255)

        gCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCA‑GTACGGCTCGCCGTACATGGTGTGaa                                                                                                                                             <  2:1062926/150‑1 (MQ=255)

            aGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATg                                                                                                                                         >  2:840129/1‑150 (MQ=255)

             gTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTCC‑TGGTGTGAAGATGc                                                                                                                                        >  1:860581/1‑150 (MQ=255)

               gCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCtt                                                                                                                                      >  1:303202/1‑150 (MQ=255)

               gCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCtt                                                                                                                                      >  1:540470/1‑150 (MQ=255)

               gCAACCGGTACCTCTTCTCGTTCAATCGGTGGCTGATACAGATGGAAAGAGTCAGGATTAACGATCGCCCGTACTGGCGTGAGAAATCACACGAATACGGTTTCAATTTTCACACCATTAACGGCGAACAGTAC‑TTGTGTGAAGATGCtt                                                                                                                                      >  1:321054/1‑150 (MQ=255)

                   ccGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAA‑TGGTGTGAAGATGCTtacta                                                                                                                                  >  1:1056242/1‑150 (MQ=255)

                     ggTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACa                                                                                                                                >  2:835719/1‑150 (MQ=255)

                     ggTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACa                                                                                                                                >  2:1152967/1‑150 (MQ=255)

                          ctctTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCTTGAGAAAGCCCACGAATACGGTTTCAATTTTAACACCATGTACGGCTAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGtag                                                                                                                           >  1:536573/1‑148 (MQ=255)

                            cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGTACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACAATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAc                                                                                                                         >  1:1101299/1‑150 (MQ=255)

                            cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAc                                                                                                                         <  2:1583558/150‑1 (MQ=255)

                            cttctCGTTCAATGGGTGGCTGATACCGATGGAAAGAGGCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGAc                                                                                                                         <  2:612405/150‑1 (MQ=255)

                                 cGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtact                                                                                                                                   >  2:1022593/1‑135 (MQ=255)

                                 cGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTtact                                                                                                                                   <  1:1022593/135‑1 (MQ=255)

                                 cGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCg                                                                                                                    >  2:817881/1‑150 (MQ=255)

                                       aTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCAAGTACGGCTCGCCGTACATGgtgt                                                                                                                                                <  1:1203231/117‑1 (MQ=255)

                                       aTGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCAAGTACGGCTCGCCGTACATGgtgt                                                                                                                                                >  2:1203231/1‑117 (MQ=255)

                                        tGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGt                                                                                                             <  1:40536/150‑1 (MQ=255)

                                         gggTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TTGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGtt                                                                                                            <  1:754871/150‑1 (MQ=255)

                                             ggCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaaa                                                                                                        <  1:840129/150‑1 (MQ=255)

                                             ggCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGaaa                                                                                                        >  2:365367/1‑150 (MQ=255)

                                              gCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc                                                                                                                                        <  1:542537/117‑1 (MQ=255)

                                              gCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGc                                                                                                                                        >  2:542537/1‑117 (MQ=255)

                                                tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGc                                                                                                     <  1:63074/150‑1 (MQ=255)

                                                tGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGc                                                                                                     <  2:130165/150‑1 (MQ=255)

                                                    aCCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGga                                                                                                 <  1:1408424/150‑1 (MQ=255)

                                                     ccGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCCCGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAG‑TGGTGGGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaa                                                                                                >  2:1057947/1‑150 (MQ=255)

                                                      cGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaag                                                                                               >  1:407660/1‑150 (MQ=255)

                                                       gATGGAAAGAGTCACTATTACCGAGCGCCCGGACTGGCTTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TTGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGgaaga                                                                                              <  1:1428188/150‑1 (MQ=255)

                                                         tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGaagaag                                                                                            <  1:365367/150‑1 (MQ=255)

                                                         tGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGaagaag                                                                                            >  1:1505637/1‑150 (MQ=255)

                                                          ggAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGt                                                                                           >  1:1342441/1‑150 (MQ=255)

                                                           gAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTc                                                                                          <  1:462917/150‑1 (MQ=255)

                                                            aaaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCa                                                                                         <  1:739583/150‑1 (MQ=255)

                                                             aaGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAc                                                                                        >  1:883360/1‑150 (MQ=255)

                                                                agTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgc                                                                                     >  1:965388/1‑150 (MQ=255)

                                                                 gTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCAccgcc                                                                                    <  2:182184/150‑1 (MQ=255)

                                                                     ttATTACAGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAAc                                                                                <  1:1011097/149‑1 (MQ=255)

                                                                     gTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAAc                                                                                >  2:49550/1‑150 (MQ=255)

                                                                      tATTACCGCGCGCCCGGACTTGCGTGAGAAAGCCCACGAATACGGTTTCTATTTTGACACCATGTAGGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTTCATGTTGAGCCGCGCCCAGGTTGTAAAGCTGGAAGATGTCACCGCCGAACt                                                                               <  2:1286298/150‑1 (MQ=255)

                                                                      tATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACt                                                                               <  1:1031038/150‑1 (MQ=255)

                                                                         tACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAGCTGcc                                                                            >  2:894014/1‑149 (MQ=255)

                                                                          aCCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAc                                                                           >  1:324616/1‑150 (MQ=255)

                                                                          aCCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCAc                                                                           >  2:460510/1‑150 (MQ=255)

                                                                            cGAGGGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCa                                                                         <  2:371330/150‑1 (MQ=255)

                                                                             gAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAg                                                                        >  1:1534100/1‑150 (MQ=255)

                                                                              aGCGCCCGGTCTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGa                                                                       <  1:1074738/150‑1 (MQ=255)

                                                                                 gcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt                                                                    <  2:1056242/150‑1 (MQ=255)

                                                                                 gcCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGAtgt                                                                    >  1:1186430/1‑150 (MQ=255)

                                                                                  cccGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCGCCAGAtgtg                                                                   >  1:363186/1‑150 (MQ=255)

                                                                                    cGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGcc                                                                 <  1:551062/150‑1 (MQ=255)

                                                                                     ggACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCt                                                                <  1:1152967/150‑1 (MQ=255)

                                                                                     ggACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCt                                                                <  1:835719/150‑1 (MQ=255)

                                                                                      gACTGGCGTTAGAAAGCCCACGAATACGTTATCAATTTTCACACCATGTACGGCTAGCCGTAC‑TGGTGTGAAGATGCTAACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCATATGTGCCTg                                                               <  1:226929/150‑1 (MQ=255)

                                                                                         tGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGaaa                                                            <  2:1286397/150‑1 (MQ=255)

                                                                                            cGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtg                                                         >  1:137664/1‑150 (MQ=255)

                                                                                             gtgtGAAAGCCCAAGAATACGGTTTCAATTTTAACACCTTGTCCTGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCACAGGTTGAAAAGCTGGAAGAAGTCACCGACGCAATTCACCAGATGTGCCTGAAAgtgg                                                        <  2:321054/150‑1 (MQ=255)

                                                                                             gTGAGAAATCCCAAGAATTAGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAA‑TTGTGTGAATATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtgg                                                        <  2:303202/150‑1 (MQ=255)

                                                                                             gTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAgtgg                                                        >  2:1625155/1‑150 (MQ=255)

                                                                                                agaAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGtggtgg                                                     <  2:783851/150‑1 (MQ=255)

                                                                                                agaAAGCCCACGAATACGGTTTCAATTTACACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTAGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGtggtgg                                                     <  2:668411/150‑1 (MQ=255)

                                                                                                  aaaGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGaa                                                   >  2:766727/1‑150 (MQ=255)

                                                                                                        cACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTg                                             >  1:318605/1‑150 (MQ=255)

                                                                                                            aaTACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCg                                         <  2:1342441/150‑1 (MQ=255)

                                                                                                            aaTACGGTTCCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCg                                         <  1:1701683/150‑1 (MQ=255)

                                                                                                               aCGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCa                                      >  1:1463093/1‑150 (MQ=255)

                                                                                                                  gTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCg                                   >  2:85071/1‑150 (MQ=255)

                                                                                                                   tttCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGa                                  >  2:575381/1‑150 (MQ=255)

                                                                                                                      cAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGCCCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGa                               >  1:1371857/1‑150 (MQ=255)

                                                                                                                      cAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGa                               >  1:1098403/1‑150 (MQ=255)

                                                                                                                        aTTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGc                             <  2:1067305/150‑1 (MQ=255)

                                                                                                                        aTTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGc                             <  2:1104196/150‑1 (MQ=255)

                                                                                                                         ttttCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCt                            <  1:985282/150‑1 (MQ=255)

                                                                                                                            tCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgat                         >  1:792385/1‑150 (MQ=255)

                                                                                                                            tCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACAGCCGAACTGCACCAGATCTGCCTGAAAGTGGTGGAAAAAGTGAACGCCAGCGATGAGCtgat                         >  1:1035836/1‑150 (MQ=255)

                                                                                                                             cacaCCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatg                        <  2:1505637/150‑1 (MQ=255)

                                                                                                                             cacaCCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatg                        <  2:1455296/150‑1 (MQ=255)

                                                                                                                              acacCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatga                       >  1:1655737/1‑150 (MQ=255)

                                                                                                                              acacCA‑GTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCtgatga                       >  1:1553588/1‑150 (MQ=255)

                                                                                                                                caccagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGAc                      >  1:15015/6‑150 (MQ=255)

                                                                                                                                 ccATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCa                    >  2:1333585/1‑150 (MQ=255)

                                                                                                                                  ccagTACGGCTCGCCGTACATGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCACAGGTTGAAAAGCTGGAAGAAGTCCCCGCCGAACTGCACCAGATGTGCCGGAAAGTGGTGGAAAATGTGATCGCCAGCGATGAGCTGATGACCa                    >  2:819261/4‑150 (MQ=255)

                                                                                                                                  ccagTACGGCTCGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGAGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCaa                   >  1:1556786/4‑150 (MQ=255)

                                                                                                                                     gTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGATAAGAAGGAAGAAGTCACCGCCGAACTGCTCCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCAGATGACCAAAtt                >  2:45928/1‑150 (MQ=255)

                                                                                                                                      tACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTc               >  2:130738/1‑150 (MQ=255)

                                                                                                                                      tACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTc               <  2:883360/150‑1 (MQ=255)

                                                                                                                                      tACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTc               >  1:388494/1‑150 (MQ=255)

                                                                                                                                          gCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCa           >  1:1032043/1‑150 (MQ=255)

                                                                                                                                             aGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTc        <  2:540470/150‑1 (MQ=255)

                                                                                                                                               ccGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGACCTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCa      >  2:710584/1‑150 (MQ=255)

                                                                                                                                               ccGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCa      >  2:1477337/1‑150 (MQ=255)

                                                                                                                                               ccGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCa      >  2:1480961/1‑150 (MQ=255)

                                                                                                                                                 gTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCaaa    >  2:23975/1‑150 (MQ=255)

ACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGGGTGGCTGATACCGATGGAAAGAGTCAGTATTACCGAGCGCCCGGACTGGCGTGAGAAAGCCCACGAATACGGTTTCAATTTTCACACCATGTACGGCGAGCCGTAC‑TGGTGTGAAGATGCTTACTACAAGTTGACCCTCGCCCAGGTTGAAAAGCTGGAAGAAGTCACCGCCGAACTGCACCAGATGTGCCTGAAAGTGGTGGAAAAAGTGATCGCCAGCGATGAGCTGATGACCAAATTCCGCATTCCAAAAC  >  NC_000913/3180365‑3180661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`