BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,648,129	C→T	5.2%	noncoding (67/84 nt)	agrA →	inactive antisense sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,648,129	0	C	T	5.2%	425.0 / 7.1	135	noncoding (67/84 nt)	agrA	inactive antisense sRNA
Reads supporting (aligned to +/- strand): ref base C (53/72); new base T (4/3); total (58/77)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

Only 100 of 138 total aligned reads displayed.

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647980‑3648278

aaCTGGAAAATCATACAGTACATGCAGGTTATAAAACCAGCCCGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGc                                                                                                                                                       >  1:1036044/1‑150 (MQ=255)

 aCTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGGCGCa                                                                                                                                                      <  2:1017016/150‑1 (MQ=255)

    ggATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTTGAAAGT‑AAAAAACCGACGCAAAg                                                                                                                                                   >  1:152285/1‑150 (MQ=255)

     gATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGt                                                                                                                                                  <  1:141506/150‑1 (MQ=255)

     gATAATCATACAGTACATGCAGGGTATAAAAGCAGCACGTCCGTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGt                                                                                                                                                  <  1:1106889/150‑1 (MQ=255)

      aTAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTc                                                                                                                                                 <  2:974889/150‑1 (MQ=255)

         aTCATACAGTACATGCAGGTTATAAAACGAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGt                                                                                                                                              <  1:1008222/150‑1 (MQ=255)

         aTCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGt                                                                                                                                              >  1:869698/1‑150 (MQ=255)

           cATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AGAAAACCGACGCAAAGTCGGttt                                                                                                                                            <  1:1550750/150‑1 (MQ=255)

             tACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACTTTGCGTCGGttttt                                                                                                                                          <  2:479114/150‑1 (MQ=38)

              aCAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTTCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         <  2:803902/150‑1 (MQ=255)

               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttttttt                                                                                                                                        <  1:631067/150‑1 (MQ=255)

               cAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTGCAGTATGGTATTCGCTTTGATGCGTTAGATGATGGCTATCTCACTCCAGGCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAACTCGGttttttt                                                                                                                                        <  1:1308250/150‑1 (MQ=37)

                aGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTa                                                                                                                                       <  1:863570/150‑1 (MQ=255)

                 gTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGTAAAAAAACCGACTTTGCGTCGGttttttac                                                                                                                                       <  1:653076/150‑3 (MQ=38)

                 gTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAc                                                                                                                                      >  2:1233783/1‑150 (MQ=255)

                     aTGCAGGTTTTAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATcc                                                                                                                                  <  1:582276/150‑1 (MQ=255)

                       gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCgg                                                                                                                                <  2:399159/150‑1 (MQ=255)

                       gCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCgg                                                                                                                                <  1:549154/150‑1 (MQ=255)

                        cAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGa                                                                                                                               >  1:1658161/1‑150 (MQ=255)

                          ggTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAtt                                                                                                                             >  1:541657/1‑150 (MQ=255)

                           gTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCAGTTTTTTTACATCCGGATTc                                                                                                                            >  1:1553705/1‑150 (MQ=255)

                            ttATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCg                                                                                                                           >  2:1186320/1‑150 (MQ=255)

                             tataAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTAAATCCGGATTCgg                                                                                                                          >  2:749844/1‑150 (MQ=255)

                                aaaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACa                                                                                                                       <  1:427338/150‑1 (MQ=255)

                                 aaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACaa                                                                                                                      <  1:783494/150‑1 (MQ=255)

                                 aaaCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACaa                                                                                                                      >  2:257844/1‑150 (MQ=255)

                                   aCCAGCACGTCCTTGCAATAGTTTCAGTATGGTATAAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGACACgg                                                                                                                    <  2:95621/150‑1 (MQ=255)

                                          cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                             <  2:479907/150‑1 (MQ=255)

                                          cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                             <  1:458456/150‑1 (MQ=255)

                                          cGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAata                                                                                                             >  1:1043325/1‑150 (MQ=255)

                                              cTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGAc                                                                                                         <  1:735086/150‑1 (MQ=255)

                                              cTTGCAATAGTTTCAGTATGGTATTAGCATTGAGGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGAc                                                                                                         <  1:850004/150‑1 (MQ=255)

                                               ttGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACGTCCGGATTCGGACATGCATTAAAATGaag                                                                                                        >  1:244910/1‑148 (MQ=21)

                                                  cAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGAAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATg                                                                                                     >  2:663776/1‑150 (MQ=255)

                                                    aTAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCTGAAAAGTATTAATATGAAGATTAc                                                                                                   >  1:1412183/1‑150 (MQ=25)

                                                       gTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCa                                                                                                >  2:743806/1‑150 (MQ=255)

                                                        tttCAGTATGGTATTAGCATTGATGCGTTAGATGATGTCTATCTCACTGCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAg                                                                                               <  1:458670/150‑1 (MQ=255)

                                                          tcatgaTGGTATTAGCATTGATGCGTTAGATGATGTCTATCTCACTCCAGTGAGTGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCTGTTTTTTTAAATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTg                                                                                             <  2:841679/145‑1 (MQ=37)

                                                            aGTTTGGTATAAGCATTGAGGCGTTAGATGAAGGCTATCTCACTCCATTCAGAGACACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGGCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaa                                                                                           <  2:814001/150‑1 (MQ=25)

                                                             gTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGaaa                                                                                          >  2:1649/1‑150 (MQ=255)

                                                                   tATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGGCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatat                                                                                    <  2:1718322/150‑1 (MQ=255)

                                                                    aTTATCATTGATGGGTTAGATTATTGCTATCTCACTCCATTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                   <  2:239774/150‑1 (MQ=25)

                                                                    aTTAGCATTGATGCGTTAGATGATGGATATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGtatata                                                                                   <  1:432032/150‑1 (MQ=255)

                                                                      tAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttt                                                                                                                                            <  1:907245/91‑1 (MQ=255)

                                                                      tAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGttt                                                                                                                                            >  2:907245/1‑91 (MQ=255)

                                                                               tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTg                                                                        >  1:143786/1‑150 (MQ=255)

                                                                               tGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCAGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACACAGTGAAAGTATATAAATCGTCACTg                                                                        <  2:1553705/150‑1 (MQ=255)

                                                                                gctttAGATGATGGCTATCTCACTCCAGTCATAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGc                                                                       <  2:326386/147‑1 (MQ=255)

                                                                                gCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGc                                                                       <  2:386771/150‑1 (MQ=255)

                                                                                   ttAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGat                                                                    >  1:868836/1‑150 (MQ=255)

                                                                                    tAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGata                                                                   <  1:339786/150‑1 (MQ=255)

                                                                                      gatgatGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         <  2:184790/78‑1 (MQ=255)

                                                                                      gatgatGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGtttttt                                                                                                                                         >  1:184790/1‑78 (MQ=255)

                                                                                         gatgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAc                                                              >  1:807226/1‑150 (MQ=255)

                                                                                         gatgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAc                                                              >  1:1521385/1‑150 (MQ=255)

                                                                                          atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAcc                                                             <  1:902287/150‑1 (MQ=255)

                                                                                          atgGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAcc                                                             <  2:245534/150‑1 (MQ=255)

                                                                                            ggCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGa                                                           >  1:219776/1‑150 (MQ=255)

                                                                                              cTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                         <  2:219776/150‑1 (MQ=255)

                                                                                              cTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAg                                                         <  2:1043325/150‑1 (MQ=255)

                                                                                               tATCTCACTCCAGTCAGAGCCACCAACTCATGGCTGGAAAGT‑AAAAAACCGACGCAAAGTAGTTTTTTTTACATCCGGATTCGGACAAGTCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCAGTGCGATATATACCGAAGt                                                        >  2:1287382/1‑150 (MQ=37)

                                                                                                aTCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                       <  2:541657/150‑1 (MQ=255)

                                                                                                aTCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                       <  2:1399933/150‑1 (MQ=255)

                                                                                                aTCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTg                                                       >  1:1108610/1‑150 (MQ=255)

                                                                                                    cACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctcc                                                   >  2:1416172/1‑150 (MQ=255)

                                                                                                     aCTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGctccc                                                  <  2:1195387/150‑1 (MQ=255)

                                                                                                         cAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCg                                              >  2:1594133/1‑150 (MQ=255)

                                                                                                               tagCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCt                                        <  1:371616/149‑1 (MQ=255)

                                                                                                                    accaACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaaga                                   <  2:1521385/150‑1 (MQ=255)

                                                                                                                     ccaACTCACGGCTGTAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTgaagaa                                  <  1:663776/150‑1 (MQ=255)

                                                                                                                        aCTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATc                               <  2:1084645/150‑1 (MQ=255)

                                                                                                                        aCTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGAATCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATc                               <  1:1266313/150‑1 (MQ=255)

                                                                                                                             gggCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTaa                          >  1:126928/1‑150 (MQ=255)

                                                                                                                             gggCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTaa                          >  1:1283767/1‑150 (MQ=255)

                                                                                                                               gCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGATCCCTCCGCCAGCTGAAGAAATCGCTAAtt                        >  2:1650837/1‑150 (MQ=255)

                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                              <  2:409487/141‑1 (MQ=255)

                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCg                              >  1:409487/1‑141 (MQ=255)

                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                     >  1:1125033/1‑150 (MQ=255)

                                                                                                                                  ggAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCtt                     >  2:959673/1‑150 (MQ=255)

                                                                                                                                   gAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTg                    <  2:152285/150‑1 (MQ=255)

                                                                                                                                    gatgtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTg                    >  1:1289487/7‑150 (MQ=255)

                                                                                                                                    aaaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGc                   <  2:866336/150‑1 (MQ=255)

                                                                                                                                     aaGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                  <  1:1529550/150‑1 (MQ=255)

                                                                                                                                     aaGT‑AAAAAAACGACGAAAAGTTGGTTTTTTTACATCAGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                  <  1:285229/150‑1 (MQ=255)

                                                                                                                                      tgtaaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCa                  >  1:765798/5‑150 (MQ=255)

                                                                                                                                        t‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCATCCGCCATCTGAAGAACTCGCTAATACTTGCAATg               >  1:320471/1‑150 (MQ=255)

                                                                                                                                         aaaaaaaCCGACTTTGCGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATg               >  2:1302715/2‑150 (MQ=255)

                                                                                                                                          aaaaaaCCGCCGCAAAGTCGGTTTTATTACATCCGGATTCGGACAAGGCTTAAGATGCCGATGACCCAGAGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTTCTCCCTCCTCAAGCAGAATAAATCGCTAAATCTTGAAATGt              >  1:269391/1‑150 (MQ=255)

                                                                                                                                            aaaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTa            >  2:580378/1‑150 (MQ=255)

                                                                                                                                             aaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAg           >  2:989627/1‑150 (MQ=255)

                                                                                                                                             aaaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATAACTAAGTGCTCCCTCCGACAGCTGAAGAAATCGCTAATTCTTGCAATGTTAg           >  1:321966/1‑150 (MQ=255)

                                                                                                                                              aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGc          >  2:763902/1‑150 (MQ=255)

                                                                                                                                              aaCCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGc          <  2:868836/150‑1 (MQ=255)

                                                                                                                                                  gACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt      <  1:257844/150‑1 (MQ=255)

                                                                                                                                                  gACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACAGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACt      >  1:383927/1‑150 (MQ=255)

                                                                                                                                                    cGCAAAGTCTGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg    <  1:689434/150‑1 (MQ=255)

                                                                                                                                                    cGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTgg    <  1:1649/150‑1 (MQ=255)

                                                                                                                                                     gCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATAGTCACTGCGATATATACCGAAGTGCTCCCTCCTCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGACACTGGc   >  1:917005/1‑150 (MQ=255)

                                                                                                                                                      cAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCt  <  2:1125033/150‑1 (MQ=255)

AACTGGATAATCATACAGTACATGCAGGTTATAAAACCAGCACGTCCTTGCAATAGTTTCAGTATGGTATTAGCATTGATGCGTTAGATGATGGCTATCTCACTCCAGTCAGAGCCACCAACTCAGGGCTGGAAAGT‑AAAAAACCGACGCAAAGTCGGTTTTTTTACATCCGGATTCGGACAAGGCTTAATATGACGATGACCCAGTGAAAGTATATAAATCGTCACTGCGATATATACCGAAGTGCTCCCTCCGCCAGCTGAAGAAATCGCTAATTCTTGCAATGTTAGCCACTGGCT  >  NC_000913/3647980‑3648278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`