Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,692,978	A→C	6.4%	L429R (CTC→CGC)	uidB ←	glucuronide transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,692,978	0	A	C	6.4%	286.5 / 4.8	95	L429R (CTC→CGC)	uidB	glucuronide transporter
Reads supporting (aligned to +/- strand):  ref base A (42/46);  new base C (2/5);  total (44/51)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAG  >  NC_000913/1692829‑1693119                                                                                                                                                       |                                                                                                                                              gCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGa                                                                                                                                               <  2:37496/150‑1 (MQ=255)     tGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCgg                                                                                                                                           >  2:1814/1‑150 (MQ=255)     tGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACAACGATTTCTTTGAA‑TTTTTTATCCGTGAGCgg                                                                                                                                           >  2:264961/1‑150 (MQ=255)       tacACTATTTTTCTAATAGTTGCACCTTTGATGTTCTTATTATTTTTATTGAATATTAATTTGTTATATCGCTGATTAATTTCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGAt                                                                                                                                         <  1:599622/148‑1 (MQ=255)        ccACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATa                                                                                                                                        <  2:928614/150‑1 (MQ=255)           cTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATaaaa                                                                                                                                     >  1:1714498/1‑150 (MQ=255)            tATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAAc                                                                                                                                    <  2:595759/150‑1 (MQ=255)            tATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGCTCACGGATaaaaa                                                                                                                                    <  1:1645170/150‑2 (MQ=255)            tATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACAATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAAc                                                                                                                                    <  1:1343500/150‑1 (MQ=255)              tttttCTCATAGTTTCTCCTTTTATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTTATTGCTGCTGCACTTTTTTACGATTTTCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGCTCACGGATaaaaaat                                                                                                                                  <  1:753242/150‑4 (MQ=255)              tttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCa                                                                                                                                  >  1:160321/1‑150 (MQ=255)              tttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCa                                                                                                                                  <  1:503060/150‑1 (MQ=255)               ttttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGCTCACGGATaaaaaatt                                                                                                                                 <  2:694781/150‑5 (MQ=255)                 ttCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGat                                                                                                                               <  1:820062/150‑1 (MQ=255)                  tctcATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGata                                                                                                                              <  2:1074807/150‑1 (MQ=255)                  tctcATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGCTCACGGATaaaaaattcaa                                                                                                                              <  1:438108/150‑8 (MQ=255)                  tctcATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGCTCACGGATaaaaaattcaa                                                                                                                              <  1:415331/150‑8 (MQ=255)                    tcATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCAGTGAGCGGATAAAACCAGataat                                                                                                                            >  1:245126/1‑150 (MQ=255)                    tcATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCAGTGAGCGGATAAAACCAGataat                                                                                                                            >  1:444102/1‑150 (MQ=255)                     cATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGataata                                                                                                                           >  2:616867/1‑150 (MQ=255)                     cATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGATGAACTTTTTTACGATTATCAATTTCAACAACGATTTCTTTGAA‑TTTTTTATCCTTGAGCTGATAAAAACAGataata                                                                                                                           >  1:1492670/1‑150 (MQ=255)                       tAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAAc                                                                                                                         <  1:924732/150‑1 (MQ=255)                        aGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACg                                                                                                                        <  2:888129/150‑1 (MQ=255)                           tGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAAt                                                                                                                     <  1:453856/150‑1 (MQ=255)                             cACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGc                                                                                                                   <  1:724924/150‑1 (MQ=255)                              aCCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGcc                                                                                                                  <  1:1048068/150‑1 (MQ=255)                              aCCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGcc                                                                                                                  <  2:1476646/150‑1 (MQ=255)                               ccTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAATTTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGcc                                                                                                                  >  1:360100/1‑150 (MQ=255)                               ccTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCa                                                                                                                 >  2:1486827/1‑150 (MQ=255)                               ccTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCAGTGAGCGGATAAAACCAGATAATAACGAATGCCa                                                                                                                 >  1:1444390/1‑150 (MQ=255)                                  ttGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTa                                                                                                              <  1:178506/150‑1 (MQ=255)                                   tGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAg                                                                                                             <  2:524375/150‑1 (MQ=255)                                     aTGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCa                                                                                                           <  1:1486827/150‑1 (MQ=255)                                       gTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATa                                                                                                         >  1:1350629/1‑150 (MQ=255)                                        ttCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATaa                                                                                                        <  1:264961/150‑1 (MQ=255)                                            gATTA‑TTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCg                                                                                                   >  2:504569/1‑150 (MQ=255)                                                        tGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTaa                                                                                        >  2:342044/1‑150 (MQ=255)                                                        tGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACAAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTaa                                                                                        >  2:1007762/1‑150 (MQ=255)                                                         gAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCTCAAGGTACTAAg                                                                                       >  2:880229/1‑150 (MQ=255)                                                          aaTATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAgg                                                                                      <  1:1664019/150‑1 (MQ=255)                                                           atatTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGc                                                                                     <  2:1231294/150‑1 (MQ=255)                                                           atatTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGc                                                                                     <  2:1714498/150‑1 (MQ=255)                                                            tatTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCa                                                                                    <  2:160186/150‑1 (MQ=255)                                                               taattaGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAAtt                                                                                 >  2:892122/1‑150 (MQ=255)                                                                    attGATATCGGAGATTAATTGATTCTTCACTTTTTTACTATTATCAATTTAAACAACGATTTCTTTGAA‑TTTTTTATACGTTAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtgt                                                                            <  2:1492670/148‑1 (MQ=255)                                                                     gTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGAtgtg                                                                           <  2:160321/150‑1 (MQ=255)                                                                      tGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGc                                                                          <  1:669022/150‑1 (MQ=255)                                                                       tatatCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCg                                                                         <  1:508938/149‑1 (MQ=255)                                                                        atatCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCgg                                                                        <  2:1196428/150‑1 (MQ=255)                                                                            cGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGc                                                                    >  2:66554/1‑150 (MQ=255)                                                                            cGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGc                                                                    <  1:1814/150‑1 (MQ=255)                                                                            cGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGc                                                                    >  1:876926/1‑150 (MQ=255)                                                                            cGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTACGGATGc                                                                    >  1:1521568/1‑150 (MQ=255)                                                                            cGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGACAGTAGAATAAATCCGCAAGGTAATAAGGCAATTGATGTGCGGATGc                                                                    >  1:62596/1‑150 (MQ=255)                                                                             gCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGcc                                                                   >  2:1155821/1‑150 (MQ=255)                                                                                   tAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCataat                                                             >  1:468714/1‑150 (MQ=255)                                                                                       tgctgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATACAACCAGATAATAACGAATGCCAGTAGCATAAATCCACAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACt                                                         >  1:97133/1‑150 (MQ=255)                                                                                        gctgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACtt                                                        >  1:992857/1‑150 (MQ=255)                                                                                         ctgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGTTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTc                                                       >  1:595367/1‑150 (MQ=255)                                                                                          tgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGCGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGGGCGGAGGCCCATAATAACTTcc                                                      >  2:1545970/1‑150 (MQ=255)                                                                                          tgctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTcc                                                      >  1:666670/1‑150 (MQ=255)                                                                                           gctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCg                                                     <  1:832509/150‑1 (MQ=255)                                                                                           gctgcACTTTTTTACGATTAACAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAAGCAGATAATAACTAATGCCAGTATCATAAATCCTAAAGGTACTAAGCCAATTGATGAGAGTATGCCCATAATAACTTCCg                                                     >  2:1242801/1‑150 (MQ=255)                                                                                            ctgcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCgg                                                    <  1:1187443/150‑1 (MQ=255)                                                                                              tcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTTAA‑TTTTTTATCCTTGAGCGGATAAAACCAGATAATAACGAATGCCATAAGCATAAATCCGCAATGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCg                                                  <  1:1235387/149‑1 (MQ=255)                                                                                              gcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCg                                                  >  2:1524447/1‑150 (MQ=255)                                                                                              gcACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCg                                                  <  2:1195862/150‑1 (MQ=255)                                                                                                 cTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGttt                                               >  2:1579721/1‑150 (MQ=255)                                                                                                 cTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAAACAGAAAATAACGAATGCCACTAGCATAAATCCGCAAGGTACTAAGGCAATTCATGTGCGGATGCCCATAATAACTTCAggtcttt                                               >  2:414873/1‑145 (MQ=255)                                                                                                  ttttttACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTg                                              <  1:29259/150‑1 (MQ=255)                                                                                                   tttttACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGc                                             >  2:809038/1‑150 (MQ=255)                                                                                                      ttACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCAcc                                          >  2:601389/1‑150 (MQ=255)                                                                                                         cGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGa                                       >  2:1498808/1‑150 (MQ=255)                                                                                                         cGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGa                                       >  1:978869/1‑150 (MQ=255)                                                                                                              aTCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGc                                  >  1:1365849/1‑150 (MQ=255)                                                                                                              aTCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGc                                  <  2:1546195/150‑1 (MQ=255)                                                                                                               tCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCg                                 <  1:1071547/150‑1 (MQ=255)                                                                                                                       aaCCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATcc                         <  2:1350629/150‑1 (MQ=255)                                                                                                                               tttCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAAcc                 <  1:342044/150‑1 (MQ=255)                                                                                                                                  cTTTGAATTTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAAcccc               <  2:360100/150‑1 (MQ=255)                                                                                                                                   tttGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCaa             >  2:1349576/1‑150 (MQ=255)                                                                                                                                      gAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTACGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGGTATATCCGCTTAACCCCAAAAt          >  1:1665133/1‑150 (MQ=255)                                                                                                                                      gAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCATAAt          >  1:999671/1‑150 (MQ=255)                                                                                                                                      gAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAAt          <  1:834999/150‑1 (MQ=255)                                                                                                                                       aa‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATa         <  1:1308555/150‑1 (MQ=255)                                                                                                                                          ttttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaaa       <  1:681564/150‑1 (MQ=255)                                                                                                                                          ttttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaaa       <  1:187391/150‑1 (MQ=255)                                                                                                                                          ggttttATCCGCTCACGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATaaa       >  1:1644108/3‑150 (MQ=255)                                                                                                                                           tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAg      >  2:756624/1‑150 (MQ=255)                                                                                                                                           tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAg      <  1:1657948/150‑1 (MQ=255)                                                                                                                                           tttttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAg      <  1:1155821/150‑1 (MQ=255)                                                                                                                                             tttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGc    <  2:245126/150‑1 (MQ=255)                                                                                                                                             tttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGc    <  2:444102/150‑1 (MQ=255)                                                                                                                                              ttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCa   <  2:978869/150‑1 (MQ=255)                                                                                                                                              ttATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCACATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCa   >  2:1206486/1‑150 (MQ=255)                                                                                                                                               tATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAg  >  2:1099273/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                              GCCATGGCCACTATTTTTCTCATAGTTGCACCTTTGATGTTCTGATTATTTTTATTGAATATTAATTAGTGATATCGCTGATTAATTGCTGCTGCACTTTTTTACGATTATCAATTTCAACCACGATTTCTTTGAA‑TTTTTTATCCGTGAGCGGATAAAACCAGATAATAACGAATGCCAGTAGCATAAATCCGCAAGGTACTAAGGCAATTGATGTGCGGATGCCCATAATAACTTCCGGCGTTTGCACCTGATTGGCGATATATCCGCTTAACCCCAAAATAAAGGCAG  >  NC_000913/1692829‑1693119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑