| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,270,605 | G→A | 7.5% | intergenic (+60/‑121) | mepS → / → rtn | murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,270,605 | 0 | G | A | 7.5% | 364.0 / 11.9 | 119 | intergenic (+60/‑121) | mepS/rtn | murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (52/58); new base A (3/6); total (55/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01 | |||||||||||
Only 100 of 121 total aligned reads displayed.
CCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACG > NC_000913/2270457‑2270754 | ccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAgg > 2:1220043/1‑150 (MQ=255) gcagTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAg < 1:1134541/150‑1 (MQ=255) cagTGGTGTTATTATTTCCAGCATGAATGAACCGTACTTGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGt < 2:1383504/150‑1 (MQ=255) agTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg < 2:26754/150‑1 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑tt > 2:1265076/1‑150 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTATGGAAGCAATCCCTTGGCTATCCTGAAGAGATAACTGAAAGCACTGCTTAGGCAGTGC‑tt > 1:1034335/1‑150 (MQ=255) tgtTATTATTTCCAGCATGAATGAAACGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttc < 1:832756/150‑2 (MQ=255) gtTATTATTTCCAGCATGAATGAACCGTACTGTAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttca < 1:345293/150‑3 (MQ=255) gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑ttttt < 1:1034340/150‑1 (MQ=255) gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttca < 1:1662262/150‑3 (MQ=255) ttattaTTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑tttttt < 1:364405/150‑1 (MQ=255) ttattaTTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttcag < 2:1332029/150‑4 (MQ=255) ttattaTTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttcag < 2:656887/150‑4 (MQ=255) tattatTTCCAGGATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttcagt < 2:761267/150‑5 (MQ=255) tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTg > 1:1269486/1‑150 (MQ=255) tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTg < 2:899376/150‑1 (MQ=255) tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTg > 2:577403/1‑150 (MQ=255) attattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGt > 2:1669733/1‑150 (MQ=255) tattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGttt < 2:1495660/150‑1 (MQ=255) tattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTGCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGttt < 1:1287656/150‑1 (MQ=255) attTCCAGCATGAATGAACCGTACTGGAATAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGtttt < 1:743255/150‑1 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttc < 1:1352348/150‑1 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttc < 2:123216/150‑1 (MQ=255) tCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttcat > 1:251144/1‑150 (MQ=255) cAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttcattc > 1:270954/1‑150 (MQ=255) aGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttcattca > 2:1292670/1‑150 (MQ=255) cATGAATGAACCGTACTTGAAGAAGCGTTACATCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATtcatc < 1:316416/150‑1 (MQ=255) atgaatgaACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCGGCAGCTAATAAACCGTTTGGTTGCAATCGCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATtcatca < 1:92099/150‑1 (MQ=255) tgaatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCag < 2:1614034/150‑1 (MQ=255) tgaatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTATTCATTCATCag > 1:9977/1‑150 (MQ=255) gaatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCaga > 2:117203/1‑150 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCagag > 1:299005/1‑150 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCagag < 1:88570/150‑1 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAgaga < 1:1140700/150‑1 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAgaga > 2:1590314/1‑150 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAgaga > 1:844000/1‑150 (MQ=255) tgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaa < 1:985683/150‑1 (MQ=255) gaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaaa > 1:1548351/1‑150 (MQ=255) aaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaaaa > 1:1549333/1‑150 (MQ=255) aaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaaaa < 1:1669733/150‑1 (MQ=255) aCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAat < 2:1195815/150‑1 (MQ=255) aCTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCCGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGt > 2:198008/1‑150 (MQ=255) ttgaagaaGCGTTACTACGAAGCACGCCGGTTTCTCAGCCGCTGCTTATAAACCGTTTGGATGCAATTCCTTGGCTTTACTGTCTAGTTATCTGAAAGCACTGCTTAGTCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGttt < 1:15401/148‑1 (MQ=255) tGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGttt > 2:450968/1‑150 (MQ=255) ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGttta > 1:227994/1‑149 (MQ=255) aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg > 1:1453234/1‑150 (MQ=255) agaagCGTTACAACGAAGAACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgc < 1:1265076/150‑1 (MQ=255) gaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgcg < 2:884519/150‑1 (MQ=255) gCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCt > 1:545516/1‑150 (MQ=255) gTTACAACGAAGCACGCCGGGTTCTCAGCCGCTTCTTATAAACCGTTTGGATTCAATCCCTTGGCTATCCTGAGGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTTGTTTTCATTCATCAGAGAATATGATGTTTGCGCGTCtt < 2:122478/150‑1 (MQ=255) cAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATcc < 2:299005/150‑1 (MQ=255) aCGAAGCAGGCCGGGTTCTCAGCCGCAGGTAATAAACCGTTTGGATGCAATCCCTTGGCTATACTGTCGAGTTAACTGAAAGCACTGGTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTTATCCAg < 2:800426/150‑1 (MQ=255) aCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAg < 2:188954/150‑1 (MQ=255) gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg > 1:1632718/1‑150 (MQ=255) cAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGt > 1:1561181/1‑150 (MQ=255) gCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCt > 1:373043/1‑150 (MQ=255) aGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCttt > 2:1482874/1‑150 (MQ=255) gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCtttt < 1:480747/150‑1 (MQ=255) cTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTa < 1:1703400/150‑1 (MQ=255) cTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTATTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTa > 1:1458470/1‑150 (MQ=255) taaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtg < 2:935260/150‑1 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt > 2:830145/1‑150 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt < 1:486410/150‑1 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt > 2:1711117/1‑150 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGCCCGGTCATTGTTATCTTTTAAAtgtt > 1:1469425/1‑150 (MQ=255) ccGTTTGGATGCAATCCCTTGGGTATCCTGTCGAGTTAACTGAAAGCAGTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCACGCTATATTCCTGTCATTGTTATCTTTTAAAtgttgt < 2:503064/150‑1 (MQ=255) ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt < 2:711156/150‑1 (MQ=255) tttGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTACGGTCATTGTTATCTTTTAAATGTTGTCGt > 1:23277/1‑150 (MQ=255) ttGGATGCAATCCCTTTGCTAACCTGACGAGATAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGGCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTa < 1:1061500/150‑1 (MQ=255) ggATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAt > 2:1051341/1‑150 (MQ=255) gCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa < 1:1292670/150‑1 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCTTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg > 2:601172/1‑150 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg < 2:1455993/150‑1 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg < 1:594696/150‑1 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGAAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg > 2:1460050/1‑150 (MQ=255) aTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGa < 2:844000/150‑1 (MQ=255) tCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGaa > 2:856193/1‑150 (MQ=255) gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg < 2:270954/150‑1 (MQ=255) gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg < 2:1548351/150‑1 (MQ=255) cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACgg > 2:1633221/1‑150 (MQ=255) tCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATTTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAAt < 1:333217/150‑1 (MQ=255) gACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg > 1:1421984/1‑133 (MQ=255) gAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc < 2:1590069/150‑1 (MQ=255) aGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa < 1:450968/150‑1 (MQ=255) aGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa > 2:402272/1‑150 (MQ=255) aGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa > 2:447078/1‑150 (MQ=255) gTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt > 1:70969/1‑150 (MQ=255) tAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc < 2:1372237/150‑1 (MQ=255) tAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc > 1:1709485/1‑150 (MQ=255) tAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTAATCAGAGAAAATGATGTTTCCGCTTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc > 2:1478668/1‑150 (MQ=255) acaaaaaaGCACTGCCTAAGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcg > 1:131287/6‑150 (MQ=255) aCTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCGGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc > 2:921401/1‑150 (MQ=255) gAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg < 2:597532/85‑1 (MQ=255) gAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg > 1:597532/1‑85 (MQ=255) cTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCGGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTg < 1:921401/150‑1 (MQ=255) tAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTggt < 2:1549333/150‑2 (MQ=255) tAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGGCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGa < 2:1034335/150‑1 (MQ=255) tAGGCAGAGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTATCCGCGTCTTGATCCAGGCTATAGTCAGGTCATTGATATCTTTTAAATGTTGTCGTAATTTCACGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGa < 2:306691/150‑1 (MQ=255) aGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAc < 1:447078/150‑1 (MQ=255) ggCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACg > 2:48568/1‑150 (MQ=255) | CCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACG > NC_000913/2270457‑2270754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |