BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,728,109	A→C	7.8%	D421E (GAT→GAG)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,728,109	0	A	C	7.8%	846.1 / 4.4	304	D421E (GAT→GAG)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base A (73/196); new base C (27/2); total (106/198)
Fisher's exact test for biased strand distribution p-value = 2.46e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

GTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC  >  NC_000913/3727960‑3728258

  ttCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑c                                                                                                                                                     <  2:635676/150‑1 (MQ=255)

   tctcGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAAGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCCGTGCTGGCCCCACCT‑cc                                                                                                                                                    >  1:4609381/1‑150 (MQ=255)

   tcacgtcgtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑cc                                                                                                                                                    >  2:3094189/4‑150 (MQ=255)

     tcgtcgTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCAT‑cccc                                                                                                                                                  >  1:624202/1‑150 (MQ=255)

     tcgtcgTGGCTGATAAGCGGCATAAAGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTAGCTGCGCCCAGTGCAGGCCCCACAA‑cccc                                                                                                                                                  >  1:5259882/1‑150 (MQ=255)

      cgtcgtGGCTGAGCAGCGGCATACCGCTGCGCATCTGGTAGAGGCGACCGGCCTAACGGTAGTTGCGGCAACCAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccccc                                                                                                                                                 <  2:1647118/150‑1 (MQ=255)

          gtGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCCCCG‑CCCCCCCCg                                                                                                                                             >  2:5332392/1‑150 (MQ=255)

            ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCggg                                                                                                                                           >  2:4937678/1‑148 (MQ=255)

             gCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCCCCGTAc                                                                                                                                          >  2:1421402/1‑150 (MQ=255)

                gATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACggg                                                                                                                                       >  2:2085/1‑149 (MQ=255)

                   aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCTCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CGCCCGCCGGGCGGtgct                                                                                                                                    >  2:6399212/1‑147 (MQ=255)

                   aaGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACgggacg                                                                                                                                    >  2:4744502/1‑146 (MQ=255)

                    aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATc                                                                                                                                   <  2:3865944/150‑1 (MQ=255)

                     gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCGCCGCCGTGGGGTGATCg                                                                                                                                  >  1:5045577/1‑150 (MQ=255)

                     gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCGCCGGGCGGtgggcg                                                                                                                                  >  1:1802863/1‑145 (MQ=255)

                     gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCg                                                                                                                                  >  1:1488893/1‑150 (MQ=255)

                        gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAAAGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCGCCCGTAGGGGAAGCGTgc                                                                                                                               >  1:113198/1‑150 (MQ=255)

                          aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                                             <  2:1758610/150‑1 (MQ=255)

                               gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc                                                                                                                        <  1:1588691/150‑1 (MQ=255)

                                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACTATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                      <  2:555982/150‑1 (MQ=255)

                                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                      <  1:3958600/150‑1 (MQ=255)

                                 tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCg                                                                                                                      <  2:1164116/150‑1 (MQ=255)

                                  gcgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCACTGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAAGCGGGctgctgcgcgc                                                                                                                     >  2:4149682/1‑145 (MQ=255)

                                   cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                    <  1:4650090/150‑1 (MQ=255)

                                   cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCt                                                                                                                    <  2:5935461/150‑1 (MQ=255)

                                    gcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTg                                                                                                                   <  1:6753505/150‑1 (MQ=255)

                                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccc                                                                                                                                                   <  2:5109258/112‑1 (MQ=255)

                                          ggTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑ccc                                                                                                                                                   >  1:5109258/1‑112 (MQ=255)

                                           gTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCg                                                                                                            <  1:6623246/150‑1 (MQ=255)

                                            tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                           <  1:5658950/150‑1 (MQ=255)

                                            tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAAGTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                                           <  1:3806525/150‑1 (MQ=255)

                                                tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                                                                                      >  1:5392454/1‑150 (MQ=255)

                                                  cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAt                                                                                                     <  2:3607313/150‑1 (MQ=255)

                                                    aCTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                                   <  1:4423303/150‑1 (MQ=255)

                                                      tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGTCATCGAGCTGCTGGCCGCGGATAGCCGCCGGCGTcggc                                                                                                 >  2:2362999/1‑147 (MQ=255)

                                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                              <  1:2219550/150‑1 (MQ=255)

                                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                              <  1:482165/150‑1 (MQ=255)

                                                         tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                                              <  2:5759213/150‑1 (MQ=255)

                                                           tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                                            <  2:1488893/150‑1 (MQ=255)

                                                              cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTa                                                                                         <  2:1092052/150‑1 (MQ=255)

                                                                gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGCCGGGCGGCGAGCGGCTGACCGGTGCTCTGCGCCGGCGGCGGGCGCCAgggcg                                                                                       >  2:4249205/1‑146 (MQ=255)

                                                                     tgctgaaacaaTTCAATGAGCTTTTTCTCTGGATTCGCCGCGATCTGCGACAGCCTTGCTGCGCCCAGTGCTGGCCACACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGATTATATCCGCCAGCATCTGTCGCCAGTACTCTCTa                                                                                  <  2:2393022/144‑1 (MQ=255)

                                                                     tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTa                                                                                  <  2:2679163/150‑1 (MQ=255)

                                                                      gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCCGTACTCACTAc                                                                                 >  2:336048/1‑150 (MQ=255)

                                                                          caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACgcgc                                                                             <  1:1006373/150‑1 (MQ=255)

                                                                          caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAG‑CCCCCCCCGGGCGGTCCTGGGGCTGCTGGGGGCGGGTGGCCGCCCGCAGCGGACGCCGGGGCTCGCTCCgcgc                                                                             >  1:2376901/1‑150 (MQ=255)

                                                                           aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcc                                                                            >  2:2508914/1‑150 (MQ=255)

                                                                                ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                                       <  1:1209007/150‑1 (MQ=255)

                                                                                ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGCCGGGACTCGCGCGGCTGACCGCGGCGCTCCGCCCGCCTCTGACGCCCGGCCGCCCTCCGcgccccgcc                                                                       >  2:1845768/1‑150 (MQ=255)

                                                                                 tCAATGAGCGAGTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgccc                                                                      <  2:4275211/150‑1 (MQ=255)

                                                                                  cAATGAGCGATTTCTCTGGAGTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCgccccgcccc                                                                     <  2:7234/150‑1 (MQ=255)

                                                                                     tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCCTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCaa                                                                  <  1:2446815/150‑1 (MQ=255)

                                                                                      gAGCGATTTCCCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                                 <  1:5730850/150‑1 (MQ=255)

                                                                                       aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTGCGGTGCGCGAGCTGCTGACCGCTGCGGGCCGCCCGCCGCTGTCGCCCGTACTCGCTACGCGCCCCGccccccagc                                                                >  2:3229843/1‑146 (MQ=255)

                                                                                         cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                                          <  1:1725462/138‑1 (MQ=255)

                                                                                          gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                             <  1:5562538/150‑1 (MQ=255)

                                                                                          gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGCTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAc                                                             <  1:1835739/150‑1 (MQ=255)

                                                                                                   ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTa                                                                                                                                           >  2:614234/1‑63 (MQ=255)

                                                                                                       tCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCg                                                <  2:6298903/150‑1 (MQ=255)

                                                                                                        cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCgg                                               <  1:3470824/150‑1 (MQ=255)

                                                                                                         gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTCCGGGGCTCGGGCTGCTGACCGCTGGTGTCCGCCAGCATCTGGCGCCAGTACTCGCTACGGGCCCCGCCCCCCGTCCGCGGAACACGTTGCGGt                                              >  2:521536/1‑150 (MQ=255)

                                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑GGCCGCCCGTACGGTAATCGTGCTGGTGTCCGGTGATTTGCGCGAGCATCTGTCGGGAGTACTGTGTAGGGGGGGCGCCCCGATTCAAGGTATGAGTTTGCGGtt                                             >  2:5710105/1‑150 (MQ=255)

                                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                             <  1:6399212/150‑1 (MQ=255)

                                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                             <  2:2334286/150‑1 (MQ=255)

                                                                                                          ccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                                             <  1:4513749/150‑1 (MQ=255)

                                                                                                           cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCG‑CCCCCCCCGTGCGGTACTCGGGCTGCTGGCCGCTGATATCCGCCGGCATCTGACGCCAGGGCTCACGACGCGCCCCGCCCCCCATCAGCGGAGCGCGTTGCggggt                                            >  1:2628052/1‑147 (MQ=255)

                                                                                                           cgcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGttt                                            <  2:371146/150‑1 (MQ=255)

                                                                                                            gcgATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACCT‑CCCCCCCCGTACGGGGGGCGCGGTGCTGACCGCTGATATCCGCCAGCCTCGGACGCCTGTACTCACTACGCGCCCCGCCCCCGCTCAACGTAACACGGTGCGGGTTa                                           >  2:442517/1‑150 (MQ=255)

                                                                                                               aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                                         >  1:3295664/1‑69 (MQ=255)

                                                                                                               aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATa                                        <  2:1300167/150‑1 (MQ=255)

                                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCGCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAAGGTAACACTTTGCGGTTTAATACCg                                     <  1:4052073/150‑1 (MQ=255)

                                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTTCGGTCATCGGGCTGCTGACCGCGGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTACCACTTTGCGGTGTAATGCCg                                     >  2:3299622/1‑150 (MQ=255)

                                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                     <  1:3117761/150‑1 (MQ=255)

                                                                                                                  tGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                                     <  1:6467772/150‑1 (MQ=255)

                                                                                                                   gcgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGc                                    <  2:1695265/150‑1 (MQ=255)

                                                                                                                       cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGc                                <  2:6436590/150‑1 (MQ=255)

                                                                                                                       cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTATTAAACCGCaaag                                <  1:3426025/150‑4 (MQ=255)

                                                                                                                          ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGGCATCGAGCTGCTGACCGCTGATCGCCGCCAGCCGCTGGCGCCAGGGCGCACTACGCGCCCCGCCCCCCAGCCCCGGCACGCTTTGCGGTTTAATACCGCCGGCagg                             >  2:687728/1‑148 (MQ=255)

                                                                                                                           cTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGc                            <  2:2457283/150‑1 (MQ=255)

                                                                                                                             tgctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc                          <  2:1537343/150‑1 (MQ=255)

                                                                                                                              gctgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg                         <  1:3632396/150‑1 (MQ=255)

                                                                                                                                tgcGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTCCCACTTTGCGGTTTAATACCGCAGGCATGCACGAc                       >  2:4517596/1‑150 (MQ=255)

                                                                                                                                 gcgcCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACa                      <  2:6609500/150‑1 (MQ=255)

                                                                                                                                    cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATGTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                   >  1:5984258/1‑150 (MQ=255)

                                                                                                                                    cccAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc                   >  2:5511415/1‑150 (MQ=255)

                                                                                                                                     ccAGTGCTGGCCCCACAT‑CCCCCCCAGTACGGTAATCGAGCTGATGACCGCTGCTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCTCATGCATGCACGACATCCa                  <  2:5073596/150‑1 (MQ=255)

                                                                                                                                      cAGTGCTGGCCCCACAG‑CCCCCCCCGTACGGGAAGCGAGCTGCTGACCGCTGCTATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCAt                 <  2:6394902/150‑1 (MQ=255)

                                                                                                                                          gCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCa             <  2:2789761/150‑1 (MQ=255)

                                                                                                                                           cTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt            >  2:6675067/1‑150 (MQ=255)

                                                                                                                                           cTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGAACGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCAt            >  2:1195222/1‑150 (MQ=255)

                                                                                                                                            tGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCATGACTCACAACGCGCCACGCCCCCAATCAAAGTACCACTTCGCGGTTTAAATCCGCACGAATGCACGACATCCAAGCCaac           >  1:209329/1‑148 (MQ=255)

                                                                                                                                            tGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATc           >  2:2096264/1‑150 (MQ=255)

                                                                                                                                              gCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCAACAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTg         >  1:2747041/1‑150 (MQ=255)

                                                                                                                                               ccccACAT‑CCCCCCCCGTACGGTAATCGTGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGTCATGCACGTCATCCATGCCATCTGc        <  2:4033535/150‑1 (MQ=255)

                                                                                                                                               ccccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATACCATCTGc        <  1:4501689/150‑1 (MQ=255)

                                                                                                                                                 ccACATCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATATGACGCCAg                                                                                           >  2:3439594/1‑66 (MQ=255)

                                                                                                                                                 ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCa      >  2:2001118/1‑150 (MQ=255)

                                                                                                                                                 ccACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCAACTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACATTGCGGTTTAATACCGCAGGCATGCACGACATCCAAGCCATCTGCCa      >  1:3715980/1‑150 (MQ=255)

                                                                                                                                                    caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg   <  1:6350861/150‑1 (MQ=255)

                                                                                                                                                    caT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAgcg   <  1:2347699/150‑1 (MQ=255)

GTTTCTCGTCGTGGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCATGCCATCTGCCAGCGC  >  NC_000913/3727960‑3728258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`