| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 291 | A→T | 6.3% | intergenic (+36/‑46) | thrL → / → thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 291 | 0 | A | T | 6.3% | 1004.8 / 42.7 | 287 | intergenic (+36/‑46) | thrL/thrA | thr operon leader peptide/Bifunctional aspartokinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (140/129); new base T (12/6); total (152/135) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.13e-01 | |||||||||||
Only 100 of 288 total aligned reads displayed.
ATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCA > NC_000913/142‑439 | aaaGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGc > 1:2498114/3‑150 (MQ=255) tcgcgcACAGACATATAAAAATTACATAGTACACAACATCCATGAAACGCATTAGCACCACCCTTCCCACCACCATCACCATTACCACAGGTAACGGTGCGGGCGGACGCTTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCg < 1:6379439/148‑1 (MQ=255) aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg < 1:5488552/150‑1 (MQ=255) aGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg < 1:4421942/150‑1 (MQ=255) aGCGCACAGAAATATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCTTACAGTAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCgg < 1:5878531/150‑1 (MQ=255) gcgcACAGACATATAAAAATTACAGACTACACAACATCCATGAAACGCATTAGCACCACCATTCCCACCACCATCACCATTACCACAGGTAAGGGTGCGGGCTGACGCGTACAGTAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCggg < 1:2130463/150‑1 (MQ=255) gcgcACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCggg < 2:5932608/150‑1 (MQ=255) cacaGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCA‑CTGTCAGGTGCGGG‑C‑tt < 1:1640864/150‑1 (MQ=255) agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑ttttttttt > 1:4536865/1‑150 (MQ=255) agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑ttttttttt > 1:4289894/1‑150 (MQ=255) agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑ttttttttt > 2:5218710/1‑150 (MQ=255) agaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑ttttttttt > 2:4989019/1‑150 (MQ=255) gaTAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTc < 1:1011453/150‑1 (MQ=255) tAAAAATTCCAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGa < 1:4381403/150‑1 (MQ=255) aTTACAGAGTACACAACATCCCTGAAACGCATTAGCACCCCCCTTACCACCCCCATCACCATTACCACAGCGAACGGGGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGGCCGCACCTGACT‑GTGCGGG‑C‑TTTTTTTTTCGACCaaa < 2:2965988/150‑1 (MQ=255) aTTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCCCCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCaaa < 2:1073545/150‑1 (MQ=255) gagTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCCAAGGTAAc > 1:2422679/1‑150 (MQ=255) ttACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGa < 1:5786263/149‑1 (MQ=255) aacaacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCTTTACCACCGGTAAGGGTGCGGGCTGACGCGTACTGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGt < 1:1227789/149‑1 (MQ=255) acaacaTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTa > 2:6626959/1‑150 (MQ=255) aTCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTaacaac > 1:6098679/1‑150 (MQ=255) ccATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa > 2:15957/1‑150 (MQ=255) cATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAt < 1:3841669/150‑1 (MQ=255) aTGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg < 1:5655875/150‑1 (MQ=255) tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc > 1:91635/1‑150 (MQ=255) tGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGc > 1:3282694/1‑150 (MQ=255) aaaCGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCgc > 2:5331141/1‑149 (MQ=255) accGCATTACCACCACCTTTACACCCCCCTCCACCATTACCCCAGGTAAGGGTGCGGGCTTACGCGTACCGTAAACACAGAAAAAAGCCGGAACCTTACC‑GTGCGGGAT‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGa < 2:3368037/148‑1 (MQ=255) gCATTCGCACCACCATTACCCCCCCCATCACCCTTACCCCAGGTAACGGGGCGGGCTGCCGCGTACAGGAAACACAGAAAAAAGCCCGCACCGGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCACAGGTAACGAGGTAACAACCATGCGAgtgt < 2:1845550/150‑1 (MQ=255) gCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑ttttttttt < 2:3753402/114‑1 (MQ=255) cATTAGCACCCCCATTACCACCACCCTCCCCATTACCCCAGGTAACGGTGCGGGCTGACGCGGACAGGAAACACCGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt < 2:5352481/150‑1 (MQ=255) cATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGtt < 1:1437123/150‑1 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg < 1:3402985/150‑1 (MQ=255) aTTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTg < 1:1490503/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGTAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:4951542/150‑1 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTACCAACCATGCGAGTGTTGaa > 1:5295842/1‑150 (MQ=255) tAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGaa < 1:4819855/150‑1 (MQ=255) aGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAg > 1:3018042/1‑150 (MQ=255) gCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGt > 1:3709483/1‑150 (MQ=255) caccacCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAATGTAAAGAGGTAACAACCATGCGAGTGTTGAAttt > 1:1301947/1‑150 (MQ=255) ccaccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcg > 1:2021907/1‑150 (MQ=255) caccaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcgg > 1:6738843/1‑150 (MQ=255) accaTTCCCACCACCATCACCATTACCACAGTTAACGGTGCGGGATGACCCTTACAGTAAACACACAAAAAACCCCCAACCTTACA‑TTGCGGG‑A‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc < 1:2848004/150‑1 (MQ=255) accaTTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc > 2:744603/1‑150 (MQ=255) ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt < 2:2499417/150‑1 (MQ=255) ccaccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACAt < 1:1939337/150‑1 (MQ=255) caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGt < 2:913033/150‑1 (MQ=255) caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAGGCGAGTGTTGAAGTTCGGCGGTACATCAGt > 2:2833003/1‑150 (MQ=255) ccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTgg > 2:4846576/1‑150 (MQ=255) catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑ttttttttttc < 2:2116983/91‑3 (MQ=255) catcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGc > 2:5825351/1‑150 (MQ=255) tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCaa > 1:5922196/1‑150 (MQ=255) tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCaa > 1:3739455/1‑150 (MQ=255) ccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGGGTTGAAGTTCGGCGGTACATCAGTGGCAAATg > 1:2150868/1‑150 (MQ=255) ttACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCCAAGGTAACGAGGTAACAACCATGCGAGTGGTGAAGTTCGGCGGTACATCAGTGGCAAATGCAg > 2:6209750/1‑150 (MQ=255) acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGAc > 1:572700/1‑80 (MQ=255) aGGTAACGGTGCGGGCTGACGCGAACAGGAAACACAGAAAAAAGCCCGCACCAGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAAAGAGGTAACAACCAAGCGAGTGGTGAAGTTCGGCGGTACATCAGTGGCAAATTCAGAACGttt > 1:2306221/1‑150 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc < 1:188258/150‑1 (MQ=255) gTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc < 2:1351075/150‑1 (MQ=255) gTGCGGGCTGACGCGTACAGGAAACACAGAATATAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCCGTGGCAAATGCAGAACGTTTTCTGCgtg > 1:3536086/1‑150 (MQ=255) gggCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGc < 1:5825379/150‑1 (MQ=255) aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGatat < 1:744603/150‑1 (MQ=255) cgTACAGGAAACACAGAAAAAATCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAAAAACCAAGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAAATCAGAAAGTTTTGTGCGTGTTTCCGATAttat > 1:6511984/1‑148 (MQ=255) gTACAGGAAACCCAGACCACAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAACGGTAACGAGGTAACACCCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAACTGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 2:5767510/150‑1 (MQ=255) gTACAGGAAACACAGAAAATAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGGCCAAAGGTAAACAGGTAACAACCATGCGAGGGTTTAAGTTCGGCGGTACATCCGTTGCAAAAGCAGAAAGTTTTGTGCGGTTTTCCGAGATTCTg > 1:4991310/1‑150 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 2:6006607/150‑1 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:1198229/150‑1 (MQ=255) gTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg < 1:6154315/150‑1 (MQ=255) tACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTgg < 2:2284072/150‑1 (MQ=255) aCAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa > 2:6090087/1‑150 (MQ=255) aCAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGa < 1:6286380/150‑1 (MQ=255) aGGAAACACAGCAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGaaa < 2:4426181/150‑1 (MQ=255) gAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGc > 2:2181571/1‑150 (MQ=255) gAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTAGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTATGGAAAGc > 1:4132860/1‑150 (MQ=255) aaaCACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑A‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCa < 2:5686481/150‑1 (MQ=255) cacaGAACAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAACGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATg < 2:40028/150‑1 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCCTGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCacggc > 2:4757632/1‑146 (MQ=255) acaGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGc > 2:3099943/1‑150 (MQ=255) aaaaaaaaaGCCCGCA‑CTGTCAGGTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCCAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGcc > 1:6508957/4‑150 (MQ=39) aGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAAGGCca > 2:6535678/1‑150 (MQ=255) aaaaaaGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg > 2:4875209/1‑150 (MQ=255) aaaaaaGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcagg > 2:3569083/1‑150 (MQ=255) aaaaaGCCCGCA‑CTGTCAGGTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggc > 2:1430145/1‑150 (MQ=255) aaaaGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggca > 2:2858154/1‑150 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGG‑CTTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcag > 2:1227077/1‑150 (MQ=255) aaGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCcaggcagg < 2:11343/150‑1 (MQ=255) aGCCCGCA‑CTGTCAGGTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAAAGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTg > 2:4548903/1‑130 (MQ=255) gCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAACGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGTCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg < 1:2638948/150‑1 (MQ=255) gCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTAGACCAAAGGTAACCAGGTAACAAACAAGCGAGTGTTTAATTTCGGCGGTACAAAACTGGCAAAATCAGAACGGTTTCTTCGTGTTTACGATATTATGGGAAACAAAGCCAggcagggg > 1:1804211/1‑150 (MQ=255) gCCCGCA‑CTGTCAGGTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcagggg > 2:1597404/1‑150 (MQ=255) cccGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt < 1:1758511/113‑1 (MQ=255) cccGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCgt > 2:1758527/1‑113 (MQ=255) ccGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGAGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggca > 2:6391427/1‑150 (MQ=255) cGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAggcaggggcag < 1:6090071/150‑1 (MQ=255) cGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCAGGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGGAAATGCAGAACGTTTTCTTCGTGTTTCCGAGAGTATGGAAATCAAAGCCAcgcaggggccg > 1:2319838/1‑148 (MQ=255) cACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGt > 2:3909164/1‑150 (MQ=255) cACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGt > 2:2227334/1‑150 (MQ=255) cACCTGACA‑GTGCGGG‑C‑TTTTTTTTTAGACCAAAGGTAACGAGGTAACAAACATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTATGCGTGTTGCCGATATTATGGAAAGCAATGCCAGGCAGGGGCAGGt > 1:51843/1‑150 (MQ=255) cTGACA‑GTGCGGG‑C‑TTTTTTTTTAGACCAAAGGTAACGAGGTAACAACCAAGCGAGTGTTGAAGTTAGGCGGTACATCAGTGGCAAAATCAGAACGTTTTATGCGTGTTGCCGATATTATGGAAAGCAATGCCAGGCAGGGGCAGGTGGc > 1:4021297/1‑150 (MQ=255) gACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCa > 2:4262832/1‑150 (MQ=255) | ATAGGCATAGCGCACAGACAGATAAAAATTACAGAGTACACAACATCCATGAAACGCATTAGCACCACCATTACCACCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACA‑GTGCGGG‑C‑TTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTCTGCGTGTTGCCGATATTCTGGAAAGCAATGCCAGGCAGGGGCAGGTGGCCA > NC_000913/142‑439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |